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G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth
SIMPLE SUMMARY: The gastrointestinal tumor microenvironment is regulated by cytokine production from the tumor and adjacent immune cells. G-CSFR is a cytokine that is highly produced in the gastrointestinal tumor microenvironment and may have unrecognized pro-tumorigenic activities. Here, we explore...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601499/ https://www.ncbi.nlm.nih.gov/pubmed/33036138 http://dx.doi.org/10.3390/cancers12102868 |
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author | Karagiannidis, Ioannis de Santana Van Vilet, Eliane Said Abu Egal, Erika Phinney, Brandon Jacenik, Damian Prossnitz, Eric R. Beswick, Ellen J. |
author_facet | Karagiannidis, Ioannis de Santana Van Vilet, Eliane Said Abu Egal, Erika Phinney, Brandon Jacenik, Damian Prossnitz, Eric R. Beswick, Ellen J. |
author_sort | Karagiannidis, Ioannis |
collection | PubMed |
description | SIMPLE SUMMARY: The gastrointestinal tumor microenvironment is regulated by cytokine production from the tumor and adjacent immune cells. G-CSFR is a cytokine that is highly produced in the gastrointestinal tumor microenvironment and may have unrecognized pro-tumorigenic activities. Here, we explored the impact of G-CSF and its receptor on macrophage responses in colon and pancreas tumors in mice. We found G-CSF/G-CSFR to promote a pro-tumorigenic macrophage response. Upon deletion of G-CSFR, macrophages exhibited increased tumor killing ability in culture and decreased tumor growth in mice. Our findings suggest that G-CSFR blockade may be beneficial to promoting anti-tumorigenic macrophage responses and should be further examined as a therapeutic target. ABSTRACT: Tumor-associated macrophages (TAMs) in the gastrointestinal tumor microenvironment (TME) are known to polarize into populations exhibiting pro- or anti-tumoral activity in response to stimuli such as growth factors and cytokines. Our previous work has recognized granulocyte colony-stimulating factor (G-CSF) as a cytokine capable of influencing immune cells of the TME exhibiting pro-tumoral activity. Here, we aimed to focus on how G-CSF regulates TAM phenotype and function and the effects on gastrointestinal (GI) tumor progression. Thus, wildtype (WT) and G-CSFR(−/−) macrophages were examined for cytokine production, gene expression, and transcription factor activity. Adoptive transfer of WT or G-CSFR(−/−) macrophages into tumor-bearing mice was performed to study their influence in the progression of colon (MC38) and pancreatic (PK5L1940) tumor mouse models. Finally, the difference in cytotoxic potential between WT and G-CSFR(−/−) macrophages was examined both in vitro and in vivo. Our results indicate that G-CSF promotes increased IL-10 production and decreased IL-12 production, which was reversed in G-CSFR(−/−) macrophages for a pro-inflammatory phenotype. Furthermore, G-CSFR(−/−) macrophages were characterized by higher levels of NOS2 expression and NO production, which led to greater tumor related cytotoxicity both in vitro and in vivo. Our results suggest that in the absence of G-CSFR, macrophage-related tumor cytotoxicity was amplified. These findings, along with our previous reports, pinpoint G-CSF /G-CSFR as a prominent target for possible clinical applications that aim to control the TME and the GI tumor progression. |
format | Online Article Text |
id | pubmed-7601499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76014992020-11-01 G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth Karagiannidis, Ioannis de Santana Van Vilet, Eliane Said Abu Egal, Erika Phinney, Brandon Jacenik, Damian Prossnitz, Eric R. Beswick, Ellen J. Cancers (Basel) Article SIMPLE SUMMARY: The gastrointestinal tumor microenvironment is regulated by cytokine production from the tumor and adjacent immune cells. G-CSFR is a cytokine that is highly produced in the gastrointestinal tumor microenvironment and may have unrecognized pro-tumorigenic activities. Here, we explored the impact of G-CSF and its receptor on macrophage responses in colon and pancreas tumors in mice. We found G-CSF/G-CSFR to promote a pro-tumorigenic macrophage response. Upon deletion of G-CSFR, macrophages exhibited increased tumor killing ability in culture and decreased tumor growth in mice. Our findings suggest that G-CSFR blockade may be beneficial to promoting anti-tumorigenic macrophage responses and should be further examined as a therapeutic target. ABSTRACT: Tumor-associated macrophages (TAMs) in the gastrointestinal tumor microenvironment (TME) are known to polarize into populations exhibiting pro- or anti-tumoral activity in response to stimuli such as growth factors and cytokines. Our previous work has recognized granulocyte colony-stimulating factor (G-CSF) as a cytokine capable of influencing immune cells of the TME exhibiting pro-tumoral activity. Here, we aimed to focus on how G-CSF regulates TAM phenotype and function and the effects on gastrointestinal (GI) tumor progression. Thus, wildtype (WT) and G-CSFR(−/−) macrophages were examined for cytokine production, gene expression, and transcription factor activity. Adoptive transfer of WT or G-CSFR(−/−) macrophages into tumor-bearing mice was performed to study their influence in the progression of colon (MC38) and pancreatic (PK5L1940) tumor mouse models. Finally, the difference in cytotoxic potential between WT and G-CSFR(−/−) macrophages was examined both in vitro and in vivo. Our results indicate that G-CSF promotes increased IL-10 production and decreased IL-12 production, which was reversed in G-CSFR(−/−) macrophages for a pro-inflammatory phenotype. Furthermore, G-CSFR(−/−) macrophages were characterized by higher levels of NOS2 expression and NO production, which led to greater tumor related cytotoxicity both in vitro and in vivo. Our results suggest that in the absence of G-CSFR, macrophage-related tumor cytotoxicity was amplified. These findings, along with our previous reports, pinpoint G-CSF /G-CSFR as a prominent target for possible clinical applications that aim to control the TME and the GI tumor progression. MDPI 2020-10-06 /pmc/articles/PMC7601499/ /pubmed/33036138 http://dx.doi.org/10.3390/cancers12102868 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Karagiannidis, Ioannis de Santana Van Vilet, Eliane Said Abu Egal, Erika Phinney, Brandon Jacenik, Damian Prossnitz, Eric R. Beswick, Ellen J. G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth |
title | G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth |
title_full | G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth |
title_fullStr | G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth |
title_full_unstemmed | G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth |
title_short | G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth |
title_sort | g-csf and g-csfr induce a pro-tumorigenic macrophage phenotype to promote colon and pancreas tumor growth |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601499/ https://www.ncbi.nlm.nih.gov/pubmed/33036138 http://dx.doi.org/10.3390/cancers12102868 |
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