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Antimicrobial Effect of Zophobas morio Hemolymph against Bovine Mastitis Pathogens
Coliforms and Staphylococcus spp. infections are the leading causes of bovine mastitis. Despite extensive research and development in antibiotics, they have remained inadequately effective in treating bovine mastitis induced by multiple pathogen infection. In the present study, we showed the protect...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601528/ https://www.ncbi.nlm.nih.gov/pubmed/32998225 http://dx.doi.org/10.3390/microorganisms8101488 |
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author | Du, Mengze Liu, Xiaodan Xu, Jiajia Li, Shuxian Wang, Shenghua Zhu, Yaohong Wang, Jiufeng |
author_facet | Du, Mengze Liu, Xiaodan Xu, Jiajia Li, Shuxian Wang, Shenghua Zhu, Yaohong Wang, Jiufeng |
author_sort | Du, Mengze |
collection | PubMed |
description | Coliforms and Staphylococcus spp. infections are the leading causes of bovine mastitis. Despite extensive research and development in antibiotics, they have remained inadequately effective in treating bovine mastitis induced by multiple pathogen infection. In the present study, we showed the protective effect of Zophobas morio (Z. morio) hemolymph on bovine mammary epithelial cells against bacterial infection. Z. morio hemolymph directly kills both Gram-positive and Gram-negative bacteria through membrane permeation and prevents the adhesion of E. coli or the clinically isolated S. simulans strain to bovine mammary epithelial (MAC-T) cells. In addition, Z. morio hemolymph downregulates the expression of nucleotide-binding oligomerization domain (NOD)-like receptor family member pyrin domain-containing protein 3 (NLRP3), caspase-1, and NLRP6, as well as inhibits the secretion of interleukin-1β (IL-1β) and IL-18, which attenuates E. coli or S. simulans-induced pyroptosis. Overall, our results suggest the potential role of Z. morio hemolymph as a novel therapeutic candidate for bovine mastitis. |
format | Online Article Text |
id | pubmed-7601528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76015282020-11-01 Antimicrobial Effect of Zophobas morio Hemolymph against Bovine Mastitis Pathogens Du, Mengze Liu, Xiaodan Xu, Jiajia Li, Shuxian Wang, Shenghua Zhu, Yaohong Wang, Jiufeng Microorganisms Article Coliforms and Staphylococcus spp. infections are the leading causes of bovine mastitis. Despite extensive research and development in antibiotics, they have remained inadequately effective in treating bovine mastitis induced by multiple pathogen infection. In the present study, we showed the protective effect of Zophobas morio (Z. morio) hemolymph on bovine mammary epithelial cells against bacterial infection. Z. morio hemolymph directly kills both Gram-positive and Gram-negative bacteria through membrane permeation and prevents the adhesion of E. coli or the clinically isolated S. simulans strain to bovine mammary epithelial (MAC-T) cells. In addition, Z. morio hemolymph downregulates the expression of nucleotide-binding oligomerization domain (NOD)-like receptor family member pyrin domain-containing protein 3 (NLRP3), caspase-1, and NLRP6, as well as inhibits the secretion of interleukin-1β (IL-1β) and IL-18, which attenuates E. coli or S. simulans-induced pyroptosis. Overall, our results suggest the potential role of Z. morio hemolymph as a novel therapeutic candidate for bovine mastitis. MDPI 2020-09-28 /pmc/articles/PMC7601528/ /pubmed/32998225 http://dx.doi.org/10.3390/microorganisms8101488 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Du, Mengze Liu, Xiaodan Xu, Jiajia Li, Shuxian Wang, Shenghua Zhu, Yaohong Wang, Jiufeng Antimicrobial Effect of Zophobas morio Hemolymph against Bovine Mastitis Pathogens |
title | Antimicrobial Effect of Zophobas morio Hemolymph against Bovine Mastitis Pathogens |
title_full | Antimicrobial Effect of Zophobas morio Hemolymph against Bovine Mastitis Pathogens |
title_fullStr | Antimicrobial Effect of Zophobas morio Hemolymph against Bovine Mastitis Pathogens |
title_full_unstemmed | Antimicrobial Effect of Zophobas morio Hemolymph against Bovine Mastitis Pathogens |
title_short | Antimicrobial Effect of Zophobas morio Hemolymph against Bovine Mastitis Pathogens |
title_sort | antimicrobial effect of zophobas morio hemolymph against bovine mastitis pathogens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601528/ https://www.ncbi.nlm.nih.gov/pubmed/32998225 http://dx.doi.org/10.3390/microorganisms8101488 |
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