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Liver Biopsy Hydroxyproline Content Is a Diagnostic for Hepatocellular Carcinoma in Murine Models of Nonalcoholic Steatohepatitis
There is increasing evidence that nonalcoholic steatohepatitis (NASH) is a risk factor for hepatocellular carcinoma (HCC) in the absence of cirrhosis, a phenomenon termed noncirrhotic HCC. Early diagnosis of HCC is critical to a favorable prognosis. We tested the hypothesis that hydroxyproline conte...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601536/ https://www.ncbi.nlm.nih.gov/pubmed/33020436 http://dx.doi.org/10.3390/diagnostics10100784 |
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author | Bissoondial, Tyler L. Han, Yiguang Mullan, Stephanie Pabla, Amrit K. Spahn, Kiera Shi, Steven Zheng, Lana Zhou, Ping Jiang, Kai Prakash, Natalia Bhutkar, Shraddha Ali, Quaisar Li, Jingsong Hu, Zhijian Pellicano, Anthony J. Goldberg, Itzhak D. Narayan, Prakash |
author_facet | Bissoondial, Tyler L. Han, Yiguang Mullan, Stephanie Pabla, Amrit K. Spahn, Kiera Shi, Steven Zheng, Lana Zhou, Ping Jiang, Kai Prakash, Natalia Bhutkar, Shraddha Ali, Quaisar Li, Jingsong Hu, Zhijian Pellicano, Anthony J. Goldberg, Itzhak D. Narayan, Prakash |
author_sort | Bissoondial, Tyler L. |
collection | PubMed |
description | There is increasing evidence that nonalcoholic steatohepatitis (NASH) is a risk factor for hepatocellular carcinoma (HCC) in the absence of cirrhosis, a phenomenon termed noncirrhotic HCC. Early diagnosis of HCC is critical to a favorable prognosis. We tested the hypothesis that hydroxyproline content of liver biopsy samples is diagnostic for HCC in murine models of NASH induced by diet or by diet and chemicals. The training set comprised mice fed a standard diet or a fast-food diet with or without administration of thioacetamide. At harvest, livers from the modified diet cohort exhibited NASH with a subset of NASH livers exhibiting HCC. Hydroxyproline content was measured in liver biopsy samples with tissue in the NASH+HCC cohort sampled from the remote, nontumor parenchyma. Plotting the receiver operating characteristics (ROC) with hydroxyproline as the continuous variable against the absence or presence of HCC yielded an area under ROC of 0.87, a threshold of >0.18 μg hydroxyproline/mg liver and sensitivity of 91% with a specificity of 83.3%. The use of liver hydroxyproline content as a diagnostic for HCC in a test set comprising healthy, NASH and NASH+HCC livers proved 87% accurate. |
format | Online Article Text |
id | pubmed-7601536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76015362020-11-01 Liver Biopsy Hydroxyproline Content Is a Diagnostic for Hepatocellular Carcinoma in Murine Models of Nonalcoholic Steatohepatitis Bissoondial, Tyler L. Han, Yiguang Mullan, Stephanie Pabla, Amrit K. Spahn, Kiera Shi, Steven Zheng, Lana Zhou, Ping Jiang, Kai Prakash, Natalia Bhutkar, Shraddha Ali, Quaisar Li, Jingsong Hu, Zhijian Pellicano, Anthony J. Goldberg, Itzhak D. Narayan, Prakash Diagnostics (Basel) Article There is increasing evidence that nonalcoholic steatohepatitis (NASH) is a risk factor for hepatocellular carcinoma (HCC) in the absence of cirrhosis, a phenomenon termed noncirrhotic HCC. Early diagnosis of HCC is critical to a favorable prognosis. We tested the hypothesis that hydroxyproline content of liver biopsy samples is diagnostic for HCC in murine models of NASH induced by diet or by diet and chemicals. The training set comprised mice fed a standard diet or a fast-food diet with or without administration of thioacetamide. At harvest, livers from the modified diet cohort exhibited NASH with a subset of NASH livers exhibiting HCC. Hydroxyproline content was measured in liver biopsy samples with tissue in the NASH+HCC cohort sampled from the remote, nontumor parenchyma. Plotting the receiver operating characteristics (ROC) with hydroxyproline as the continuous variable against the absence or presence of HCC yielded an area under ROC of 0.87, a threshold of >0.18 μg hydroxyproline/mg liver and sensitivity of 91% with a specificity of 83.3%. The use of liver hydroxyproline content as a diagnostic for HCC in a test set comprising healthy, NASH and NASH+HCC livers proved 87% accurate. MDPI 2020-10-04 /pmc/articles/PMC7601536/ /pubmed/33020436 http://dx.doi.org/10.3390/diagnostics10100784 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bissoondial, Tyler L. Han, Yiguang Mullan, Stephanie Pabla, Amrit K. Spahn, Kiera Shi, Steven Zheng, Lana Zhou, Ping Jiang, Kai Prakash, Natalia Bhutkar, Shraddha Ali, Quaisar Li, Jingsong Hu, Zhijian Pellicano, Anthony J. Goldberg, Itzhak D. Narayan, Prakash Liver Biopsy Hydroxyproline Content Is a Diagnostic for Hepatocellular Carcinoma in Murine Models of Nonalcoholic Steatohepatitis |
title | Liver Biopsy Hydroxyproline Content Is a Diagnostic for Hepatocellular Carcinoma in Murine Models of Nonalcoholic Steatohepatitis |
title_full | Liver Biopsy Hydroxyproline Content Is a Diagnostic for Hepatocellular Carcinoma in Murine Models of Nonalcoholic Steatohepatitis |
title_fullStr | Liver Biopsy Hydroxyproline Content Is a Diagnostic for Hepatocellular Carcinoma in Murine Models of Nonalcoholic Steatohepatitis |
title_full_unstemmed | Liver Biopsy Hydroxyproline Content Is a Diagnostic for Hepatocellular Carcinoma in Murine Models of Nonalcoholic Steatohepatitis |
title_short | Liver Biopsy Hydroxyproline Content Is a Diagnostic for Hepatocellular Carcinoma in Murine Models of Nonalcoholic Steatohepatitis |
title_sort | liver biopsy hydroxyproline content is a diagnostic for hepatocellular carcinoma in murine models of nonalcoholic steatohepatitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601536/ https://www.ncbi.nlm.nih.gov/pubmed/33020436 http://dx.doi.org/10.3390/diagnostics10100784 |
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