Cargando…
Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome
Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601643/ https://www.ncbi.nlm.nih.gov/pubmed/33065969 http://dx.doi.org/10.3390/plants9101350 |
_version_ | 1783603475598278656 |
---|---|
author | Wang, Tao Jia, Zhan-Hui Zhang, Ji-Yu Liu, Min Guo, Zhong-Ren Wang, Gang |
author_facet | Wang, Tao Jia, Zhan-Hui Zhang, Ji-Yu Liu, Min Guo, Zhong-Ren Wang, Gang |
author_sort | Wang, Tao |
collection | PubMed |
description | Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the present study, we identified 100 NBS-LRR genes in the kiwifruit genome and they were grouped into six distinct classes based on their domain architecture. Of the 100 genes, 79 are truncated non-regular NBS-LRR genes. Except for 37 NBS-LRR genes with no location information, the remaining 63 genes are distributed unevenly across 18 kiwifruit chromosomes and 38.01% of them are present in clusters. Seventeen families of cis-acting elements were identified in the promoters of the NBS-LRR genes, including AP2, NAC, ERF and MYB. Pseudomonas syringae pv. actinidiae (pathogen of the kiwifruit bacterial canker) infection induced differential expressions of 16 detected NBS-LRR genes and three of them are involved in plant immunity responses. Our study provides insight of the NBS-LRR genes in kiwifruit and a resource for the identification of new R-genes in the fruit. |
format | Online Article Text |
id | pubmed-7601643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76016432020-11-01 Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome Wang, Tao Jia, Zhan-Hui Zhang, Ji-Yu Liu, Min Guo, Zhong-Ren Wang, Gang Plants (Basel) Article Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the present study, we identified 100 NBS-LRR genes in the kiwifruit genome and they were grouped into six distinct classes based on their domain architecture. Of the 100 genes, 79 are truncated non-regular NBS-LRR genes. Except for 37 NBS-LRR genes with no location information, the remaining 63 genes are distributed unevenly across 18 kiwifruit chromosomes and 38.01% of them are present in clusters. Seventeen families of cis-acting elements were identified in the promoters of the NBS-LRR genes, including AP2, NAC, ERF and MYB. Pseudomonas syringae pv. actinidiae (pathogen of the kiwifruit bacterial canker) infection induced differential expressions of 16 detected NBS-LRR genes and three of them are involved in plant immunity responses. Our study provides insight of the NBS-LRR genes in kiwifruit and a resource for the identification of new R-genes in the fruit. MDPI 2020-10-13 /pmc/articles/PMC7601643/ /pubmed/33065969 http://dx.doi.org/10.3390/plants9101350 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Tao Jia, Zhan-Hui Zhang, Ji-Yu Liu, Min Guo, Zhong-Ren Wang, Gang Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome |
title | Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome |
title_full | Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome |
title_fullStr | Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome |
title_full_unstemmed | Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome |
title_short | Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome |
title_sort | identification and analysis of nbs-lrr genes in actinidia chinensis genome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601643/ https://www.ncbi.nlm.nih.gov/pubmed/33065969 http://dx.doi.org/10.3390/plants9101350 |
work_keys_str_mv | AT wangtao identificationandanalysisofnbslrrgenesinactinidiachinensisgenome AT jiazhanhui identificationandanalysisofnbslrrgenesinactinidiachinensisgenome AT zhangjiyu identificationandanalysisofnbslrrgenesinactinidiachinensisgenome AT liumin identificationandanalysisofnbslrrgenesinactinidiachinensisgenome AT guozhongren identificationandanalysisofnbslrrgenesinactinidiachinensisgenome AT wanggang identificationandanalysisofnbslrrgenesinactinidiachinensisgenome |