Cargando…

Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome

Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Tao, Jia, Zhan-Hui, Zhang, Ji-Yu, Liu, Min, Guo, Zhong-Ren, Wang, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601643/
https://www.ncbi.nlm.nih.gov/pubmed/33065969
http://dx.doi.org/10.3390/plants9101350
_version_ 1783603475598278656
author Wang, Tao
Jia, Zhan-Hui
Zhang, Ji-Yu
Liu, Min
Guo, Zhong-Ren
Wang, Gang
author_facet Wang, Tao
Jia, Zhan-Hui
Zhang, Ji-Yu
Liu, Min
Guo, Zhong-Ren
Wang, Gang
author_sort Wang, Tao
collection PubMed
description Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the present study, we identified 100 NBS-LRR genes in the kiwifruit genome and they were grouped into six distinct classes based on their domain architecture. Of the 100 genes, 79 are truncated non-regular NBS-LRR genes. Except for 37 NBS-LRR genes with no location information, the remaining 63 genes are distributed unevenly across 18 kiwifruit chromosomes and 38.01% of them are present in clusters. Seventeen families of cis-acting elements were identified in the promoters of the NBS-LRR genes, including AP2, NAC, ERF and MYB. Pseudomonas syringae pv. actinidiae (pathogen of the kiwifruit bacterial canker) infection induced differential expressions of 16 detected NBS-LRR genes and three of them are involved in plant immunity responses. Our study provides insight of the NBS-LRR genes in kiwifruit and a resource for the identification of new R-genes in the fruit.
format Online
Article
Text
id pubmed-7601643
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76016432020-11-01 Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome Wang, Tao Jia, Zhan-Hui Zhang, Ji-Yu Liu, Min Guo, Zhong-Ren Wang, Gang Plants (Basel) Article Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the present study, we identified 100 NBS-LRR genes in the kiwifruit genome and they were grouped into six distinct classes based on their domain architecture. Of the 100 genes, 79 are truncated non-regular NBS-LRR genes. Except for 37 NBS-LRR genes with no location information, the remaining 63 genes are distributed unevenly across 18 kiwifruit chromosomes and 38.01% of them are present in clusters. Seventeen families of cis-acting elements were identified in the promoters of the NBS-LRR genes, including AP2, NAC, ERF and MYB. Pseudomonas syringae pv. actinidiae (pathogen of the kiwifruit bacterial canker) infection induced differential expressions of 16 detected NBS-LRR genes and three of them are involved in plant immunity responses. Our study provides insight of the NBS-LRR genes in kiwifruit and a resource for the identification of new R-genes in the fruit. MDPI 2020-10-13 /pmc/articles/PMC7601643/ /pubmed/33065969 http://dx.doi.org/10.3390/plants9101350 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Tao
Jia, Zhan-Hui
Zhang, Ji-Yu
Liu, Min
Guo, Zhong-Ren
Wang, Gang
Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome
title Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome
title_full Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome
title_fullStr Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome
title_full_unstemmed Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome
title_short Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome
title_sort identification and analysis of nbs-lrr genes in actinidia chinensis genome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601643/
https://www.ncbi.nlm.nih.gov/pubmed/33065969
http://dx.doi.org/10.3390/plants9101350
work_keys_str_mv AT wangtao identificationandanalysisofnbslrrgenesinactinidiachinensisgenome
AT jiazhanhui identificationandanalysisofnbslrrgenesinactinidiachinensisgenome
AT zhangjiyu identificationandanalysisofnbslrrgenesinactinidiachinensisgenome
AT liumin identificationandanalysisofnbslrrgenesinactinidiachinensisgenome
AT guozhongren identificationandanalysisofnbslrrgenesinactinidiachinensisgenome
AT wanggang identificationandanalysisofnbslrrgenesinactinidiachinensisgenome