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Emergence and Spread of Piscine orthoreovirus Genotype 3

Piscine orthoreovirus (PRV) is a relevant pathogen for salmonid aquaculture worldwide. In 2015, a new genotype of PRV (genotype 3, PRV-3) was discovered in Norway, and in 2017 PRV-3 was detected for first time in Denmark in association with complex disease cases in rainbow trout in recirculating aqu...

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Autores principales: Sørensen, Juliane, Vendramin, Niccolò, Priess, Camilla, Kannimuthu, Dhamotharan, Henriksen, Niels Henrik, Iburg, Tine Moesgaard, Olesen, Niels Jørgen, Cuenca, Argelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601675/
https://www.ncbi.nlm.nih.gov/pubmed/33036449
http://dx.doi.org/10.3390/pathogens9100823
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author Sørensen, Juliane
Vendramin, Niccolò
Priess, Camilla
Kannimuthu, Dhamotharan
Henriksen, Niels Henrik
Iburg, Tine Moesgaard
Olesen, Niels Jørgen
Cuenca, Argelia
author_facet Sørensen, Juliane
Vendramin, Niccolò
Priess, Camilla
Kannimuthu, Dhamotharan
Henriksen, Niels Henrik
Iburg, Tine Moesgaard
Olesen, Niels Jørgen
Cuenca, Argelia
author_sort Sørensen, Juliane
collection PubMed
description Piscine orthoreovirus (PRV) is a relevant pathogen for salmonid aquaculture worldwide. In 2015, a new genotype of PRV (genotype 3, PRV-3) was discovered in Norway, and in 2017 PRV-3 was detected for first time in Denmark in association with complex disease cases in rainbow trout in recirculating aquaculture systems (RAS). To explore the epidemiology of PRV-3 in Denmark, a surveillance study was conducted in 2017 to 2019. Fifty-three farms, including both flow through and RAS, were screened for PRV-3. Of the farms examined, PRV-3 was detected in thirty-eight (71.7%), with the highest prevalence in grow-out farms. Notably, in Denmark disease outbreaks were only observed in RAS. Additionally, wild Atlantic salmon and brown trout populations were included in the screening, and PRV-3 was not detected in the three years where samples were obtained (2016, 2018, and 2019). Historical samples in the form of archived material at the Danish National Reference Laboratory for Fish Diseases were also tested for the presence of PRV-3, allowing us to establish that the virus has been present in Denmark at least since 1995. Sequence analyses of segment S1 and M2, as well as full genome analyses of selected isolates, did not reveal clear association between genetic makeup in these two segments and virulence in the form of disease outbreaks in the field.
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spelling pubmed-76016752020-11-01 Emergence and Spread of Piscine orthoreovirus Genotype 3 Sørensen, Juliane Vendramin, Niccolò Priess, Camilla Kannimuthu, Dhamotharan Henriksen, Niels Henrik Iburg, Tine Moesgaard Olesen, Niels Jørgen Cuenca, Argelia Pathogens Article Piscine orthoreovirus (PRV) is a relevant pathogen for salmonid aquaculture worldwide. In 2015, a new genotype of PRV (genotype 3, PRV-3) was discovered in Norway, and in 2017 PRV-3 was detected for first time in Denmark in association with complex disease cases in rainbow trout in recirculating aquaculture systems (RAS). To explore the epidemiology of PRV-3 in Denmark, a surveillance study was conducted in 2017 to 2019. Fifty-three farms, including both flow through and RAS, were screened for PRV-3. Of the farms examined, PRV-3 was detected in thirty-eight (71.7%), with the highest prevalence in grow-out farms. Notably, in Denmark disease outbreaks were only observed in RAS. Additionally, wild Atlantic salmon and brown trout populations were included in the screening, and PRV-3 was not detected in the three years where samples were obtained (2016, 2018, and 2019). Historical samples in the form of archived material at the Danish National Reference Laboratory for Fish Diseases were also tested for the presence of PRV-3, allowing us to establish that the virus has been present in Denmark at least since 1995. Sequence analyses of segment S1 and M2, as well as full genome analyses of selected isolates, did not reveal clear association between genetic makeup in these two segments and virulence in the form of disease outbreaks in the field. MDPI 2020-10-07 /pmc/articles/PMC7601675/ /pubmed/33036449 http://dx.doi.org/10.3390/pathogens9100823 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sørensen, Juliane
Vendramin, Niccolò
Priess, Camilla
Kannimuthu, Dhamotharan
Henriksen, Niels Henrik
Iburg, Tine Moesgaard
Olesen, Niels Jørgen
Cuenca, Argelia
Emergence and Spread of Piscine orthoreovirus Genotype 3
title Emergence and Spread of Piscine orthoreovirus Genotype 3
title_full Emergence and Spread of Piscine orthoreovirus Genotype 3
title_fullStr Emergence and Spread of Piscine orthoreovirus Genotype 3
title_full_unstemmed Emergence and Spread of Piscine orthoreovirus Genotype 3
title_short Emergence and Spread of Piscine orthoreovirus Genotype 3
title_sort emergence and spread of piscine orthoreovirus genotype 3
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601675/
https://www.ncbi.nlm.nih.gov/pubmed/33036449
http://dx.doi.org/10.3390/pathogens9100823
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