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Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice

Obesity and obesity-related disorders, such as type 2 diabetes have been progressively increasing worldwide and treatments have failed to counteract their progression. Growing evidence have demonstrated that gut microbiota is associated with the incidence of these pathologies. Hence, the identificat...

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Autores principales: Régnier, Marion, Rastelli, Marialetizia, Morissette, Arianne, Suriano, Francesco, Le Roy, Tiphaine, Pilon, Geneviève, Delzenne, Nathalie M, Marette, André, Van Hul, Matthias, Cani, Patrice D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601677/
https://www.ncbi.nlm.nih.gov/pubmed/32987923
http://dx.doi.org/10.3390/nu12102932
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author Régnier, Marion
Rastelli, Marialetizia
Morissette, Arianne
Suriano, Francesco
Le Roy, Tiphaine
Pilon, Geneviève
Delzenne, Nathalie M
Marette, André
Van Hul, Matthias
Cani, Patrice D
author_facet Régnier, Marion
Rastelli, Marialetizia
Morissette, Arianne
Suriano, Francesco
Le Roy, Tiphaine
Pilon, Geneviève
Delzenne, Nathalie M
Marette, André
Van Hul, Matthias
Cani, Patrice D
author_sort Régnier, Marion
collection PubMed
description Obesity and obesity-related disorders, such as type 2 diabetes have been progressively increasing worldwide and treatments have failed to counteract their progression. Growing evidence have demonstrated that gut microbiota is associated with the incidence of these pathologies. Hence, the identification of new nutritional compounds, able to improve health through a modulation of gut microbiota, is gaining interest. In this context, the aim of this study was to investigate the gut-driving effects of rhubarb extract in a context of diet-induced obesity and diabetes. Eight weeks old C57BL6/J male mice were fed a control diet (CTRL), a high fat and high sucrose diet (HFHS) or a HFHS diet supplemented with 0.3% (g/g) of rhubarb extract for eight weeks. Rhubarb supplementation fully prevented HFHS-induced obesity, diabetes, visceral adiposity, adipose tissue inflammation and liver triglyceride accumulation, without any modification in food intake. By combining sequencing and qPCR methods, we found that all these effects were associated with a blooming of Akkermansia muciniphila, which is strongly correlated with increased expression of Reg3γ in the colon. Our data showed that rhubarb supplementation is sufficient to protect against metabolic disorders induced by a diet rich in lipid and carbohydrates in association with a reciprocal interaction between Akkermansia muciniphila and Reg3γ.
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spelling pubmed-76016772020-11-01 Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice Régnier, Marion Rastelli, Marialetizia Morissette, Arianne Suriano, Francesco Le Roy, Tiphaine Pilon, Geneviève Delzenne, Nathalie M Marette, André Van Hul, Matthias Cani, Patrice D Nutrients Article Obesity and obesity-related disorders, such as type 2 diabetes have been progressively increasing worldwide and treatments have failed to counteract their progression. Growing evidence have demonstrated that gut microbiota is associated with the incidence of these pathologies. Hence, the identification of new nutritional compounds, able to improve health through a modulation of gut microbiota, is gaining interest. In this context, the aim of this study was to investigate the gut-driving effects of rhubarb extract in a context of diet-induced obesity and diabetes. Eight weeks old C57BL6/J male mice were fed a control diet (CTRL), a high fat and high sucrose diet (HFHS) or a HFHS diet supplemented with 0.3% (g/g) of rhubarb extract for eight weeks. Rhubarb supplementation fully prevented HFHS-induced obesity, diabetes, visceral adiposity, adipose tissue inflammation and liver triglyceride accumulation, without any modification in food intake. By combining sequencing and qPCR methods, we found that all these effects were associated with a blooming of Akkermansia muciniphila, which is strongly correlated with increased expression of Reg3γ in the colon. Our data showed that rhubarb supplementation is sufficient to protect against metabolic disorders induced by a diet rich in lipid and carbohydrates in association with a reciprocal interaction between Akkermansia muciniphila and Reg3γ. MDPI 2020-09-24 /pmc/articles/PMC7601677/ /pubmed/32987923 http://dx.doi.org/10.3390/nu12102932 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Régnier, Marion
Rastelli, Marialetizia
Morissette, Arianne
Suriano, Francesco
Le Roy, Tiphaine
Pilon, Geneviève
Delzenne, Nathalie M
Marette, André
Van Hul, Matthias
Cani, Patrice D
Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice
title Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice
title_full Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice
title_fullStr Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice
title_full_unstemmed Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice
title_short Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice
title_sort rhubarb supplementation prevents diet-induced obesity and diabetes in association with increased akkermansia muciniphila in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601677/
https://www.ncbi.nlm.nih.gov/pubmed/32987923
http://dx.doi.org/10.3390/nu12102932
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