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Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion

Objectives: Retinal vein occlusions (RVO) are associated with systemic risk factors. However, the ocular occlusive events might also influence a patient’s systemic condition. This study tried to investigate serum biomarkers associated with oxidative stress, before and after intravitreal anti-vascula...

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Autores principales: Hwang, De-Kuang, Chang, Yuh-Lih, Lin, Tai-Chi, Peng, Chi-Hsien, Chien, Ke-Hung, Tsai, Ching-Yao, Chen, Shih-Jen, Chen, Kuan-Hsuan, Hsu, Min-Yen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601748/
https://www.ncbi.nlm.nih.gov/pubmed/33036304
http://dx.doi.org/10.3390/biom10101414
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author Hwang, De-Kuang
Chang, Yuh-Lih
Lin, Tai-Chi
Peng, Chi-Hsien
Chien, Ke-Hung
Tsai, Ching-Yao
Chen, Shih-Jen
Chen, Kuan-Hsuan
Hsu, Min-Yen
author_facet Hwang, De-Kuang
Chang, Yuh-Lih
Lin, Tai-Chi
Peng, Chi-Hsien
Chien, Ke-Hung
Tsai, Ching-Yao
Chen, Shih-Jen
Chen, Kuan-Hsuan
Hsu, Min-Yen
author_sort Hwang, De-Kuang
collection PubMed
description Objectives: Retinal vein occlusions (RVO) are associated with systemic risk factors. However, the ocular occlusive events might also influence a patient’s systemic condition. This study tried to investigate serum biomarkers associated with oxidative stress, before and after intravitreal anti-vascular endothelial growth factor (aVEGF) therapy in patients with RVOs. Methods: Newly-onset RVO patients were categorized into two groups: comorbid with macular edema requiring aVEGF therapy (treatment group) and no edema (observation group). Age and sex-matched patients (who received cataract surgery) were included as the control group. Intravitreal ranibizumab with a pro-re-nata regimen were administered. Serum samples were collected prior to treatment, at 6 and 12 months after therapy/observation and were collected once before controls who received cataract surgery. mRNA expression of sirtuin-1, its downstream genes, anti-oxidative biomarkers, and proinflammatory cytokines were measured. Results: There were 32, 26, and 34 patients enrolled in the treatment, observation, and control groups, respectively. The expressions of sirtuin-1 and its downstream genes were significantly lower in patients with RVO compared with the control group. Sirtuin-1 gene expression increased after 1 year of aVEGF therapy in the treatment group but remained unchanged in the observation group. Biomarkers of oxidative stress and proinflammatory cytokines were reduced after 1 year of aVEGF therapy. These biomarkers remained with no changes in the observation group. Conclusions: Our study showed that the systemic oxidative stress increased in RVO patients. The aVEGF therapy could alter the gene expression of anti-oxidative proteins and reduce systemic oxidative stress in these patients.
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spelling pubmed-76017482020-11-01 Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion Hwang, De-Kuang Chang, Yuh-Lih Lin, Tai-Chi Peng, Chi-Hsien Chien, Ke-Hung Tsai, Ching-Yao Chen, Shih-Jen Chen, Kuan-Hsuan Hsu, Min-Yen Biomolecules Article Objectives: Retinal vein occlusions (RVO) are associated with systemic risk factors. However, the ocular occlusive events might also influence a patient’s systemic condition. This study tried to investigate serum biomarkers associated with oxidative stress, before and after intravitreal anti-vascular endothelial growth factor (aVEGF) therapy in patients with RVOs. Methods: Newly-onset RVO patients were categorized into two groups: comorbid with macular edema requiring aVEGF therapy (treatment group) and no edema (observation group). Age and sex-matched patients (who received cataract surgery) were included as the control group. Intravitreal ranibizumab with a pro-re-nata regimen were administered. Serum samples were collected prior to treatment, at 6 and 12 months after therapy/observation and were collected once before controls who received cataract surgery. mRNA expression of sirtuin-1, its downstream genes, anti-oxidative biomarkers, and proinflammatory cytokines were measured. Results: There were 32, 26, and 34 patients enrolled in the treatment, observation, and control groups, respectively. The expressions of sirtuin-1 and its downstream genes were significantly lower in patients with RVO compared with the control group. Sirtuin-1 gene expression increased after 1 year of aVEGF therapy in the treatment group but remained unchanged in the observation group. Biomarkers of oxidative stress and proinflammatory cytokines were reduced after 1 year of aVEGF therapy. These biomarkers remained with no changes in the observation group. Conclusions: Our study showed that the systemic oxidative stress increased in RVO patients. The aVEGF therapy could alter the gene expression of anti-oxidative proteins and reduce systemic oxidative stress in these patients. MDPI 2020-10-06 /pmc/articles/PMC7601748/ /pubmed/33036304 http://dx.doi.org/10.3390/biom10101414 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hwang, De-Kuang
Chang, Yuh-Lih
Lin, Tai-Chi
Peng, Chi-Hsien
Chien, Ke-Hung
Tsai, Ching-Yao
Chen, Shih-Jen
Chen, Kuan-Hsuan
Hsu, Min-Yen
Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion
title Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion
title_full Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion
title_fullStr Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion
title_full_unstemmed Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion
title_short Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion
title_sort changes in the systemic expression of sirtuin-1 and oxidative stress after intravitreal anti-vascular endothelial growth factor in patients with retinal vein occlusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601748/
https://www.ncbi.nlm.nih.gov/pubmed/33036304
http://dx.doi.org/10.3390/biom10101414
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