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Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection

The highly sensitive interfacial effects between liquid crystal (LC) and alignment layers make LC-bioinspired sensors an important technology. However, LC-bioinspired sensors are limited by quantification requiring a polarized microscope and expensive equipment, which makes it difficult to commercia...

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Autores principales: Huang, Haw-Ming, Chuang, Er-Yuan, Chen, Fu-Lun, Lin, Jia-De, Hsiao, Yu-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601757/
https://www.ncbi.nlm.nih.gov/pubmed/33036425
http://dx.doi.org/10.3390/polym12102294
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author Huang, Haw-Ming
Chuang, Er-Yuan
Chen, Fu-Lun
Lin, Jia-De
Hsiao, Yu-Cheng
author_facet Huang, Haw-Ming
Chuang, Er-Yuan
Chen, Fu-Lun
Lin, Jia-De
Hsiao, Yu-Cheng
author_sort Huang, Haw-Ming
collection PubMed
description The highly sensitive interfacial effects between liquid crystal (LC) and alignment layers make LC-bioinspired sensors an important technology. However, LC-bioinspired sensors are limited by quantification requiring a polarized microscope and expensive equipment, which makes it difficult to commercialize LC-bioinspired sensors. In this report, we first demonstrate that dye-doped LC (DDLC) chips coated with vertically aligned layers can be employed as a new LC-bioinspired sensing technology. The DDLC-bioinspired sensor was tested by detecting bovine serum albumin (BSA) and immunocomplexes of BSA pairs. The intensities of the dye color of the DDLC-bioinspired sensor can be changed with the concentrations of biomolecules and immunocomplexes. A detection limit of 0.5 µg/mL was shown for the color-indicating DDLC-bioinspired sensors. We also designed a new method to use the quantitative DDLC-bioinspired sensor with a smart-phone for potential of home test. The novel DDLC-bioinspired sensor is cheap, label-free, and easy to use, furthering the technology for home and field-based disease-related detection.
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spelling pubmed-76017572020-11-01 Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection Huang, Haw-Ming Chuang, Er-Yuan Chen, Fu-Lun Lin, Jia-De Hsiao, Yu-Cheng Polymers (Basel) Article The highly sensitive interfacial effects between liquid crystal (LC) and alignment layers make LC-bioinspired sensors an important technology. However, LC-bioinspired sensors are limited by quantification requiring a polarized microscope and expensive equipment, which makes it difficult to commercialize LC-bioinspired sensors. In this report, we first demonstrate that dye-doped LC (DDLC) chips coated with vertically aligned layers can be employed as a new LC-bioinspired sensing technology. The DDLC-bioinspired sensor was tested by detecting bovine serum albumin (BSA) and immunocomplexes of BSA pairs. The intensities of the dye color of the DDLC-bioinspired sensor can be changed with the concentrations of biomolecules and immunocomplexes. A detection limit of 0.5 µg/mL was shown for the color-indicating DDLC-bioinspired sensors. We also designed a new method to use the quantitative DDLC-bioinspired sensor with a smart-phone for potential of home test. The novel DDLC-bioinspired sensor is cheap, label-free, and easy to use, furthering the technology for home and field-based disease-related detection. MDPI 2020-10-07 /pmc/articles/PMC7601757/ /pubmed/33036425 http://dx.doi.org/10.3390/polym12102294 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Haw-Ming
Chuang, Er-Yuan
Chen, Fu-Lun
Lin, Jia-De
Hsiao, Yu-Cheng
Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection
title Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection
title_full Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection
title_fullStr Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection
title_full_unstemmed Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection
title_short Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection
title_sort color-indicating, label-free, dye-doped liquid crystal organic-polymer-based-bioinspired sensor for biomolecule immunodetection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601757/
https://www.ncbi.nlm.nih.gov/pubmed/33036425
http://dx.doi.org/10.3390/polym12102294
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