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Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions
SIMPLE SUMMARY: The cryopreservation and long-term storage of semen is one of the methods for accelerated improvement of the genetic qualities of animals. However, horse breeders prefer to use fresh or chilled semen, as the fertilizing capacity of frozen equine semen is much lower. It is important t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601834/ https://www.ncbi.nlm.nih.gov/pubmed/33020383 http://dx.doi.org/10.3390/ani10101801 |
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author | Nikitkina, Elena Musidray, Artem Krutikova, Anna Anipchenko, Polina Plemyashov, Kirill Shiryaev, Gennadiy |
author_facet | Nikitkina, Elena Musidray, Artem Krutikova, Anna Anipchenko, Polina Plemyashov, Kirill Shiryaev, Gennadiy |
author_sort | Nikitkina, Elena |
collection | PubMed |
description | SIMPLE SUMMARY: The cryopreservation and long-term storage of semen is one of the methods for accelerated improvement of the genetic qualities of animals. However, horse breeders prefer to use fresh or chilled semen, as the fertilizing capacity of frozen equine semen is much lower. It is important to find extenders, or a combination of extenders, that will improve semen survival after freezing. It is also important that the extender can be easily and simply prepared for use. Steridyl is a concentrate to which you just need to add sterilized water. This extender was developed for ruminants. In this study we tested Steridyl for freezing stallion semen. The motility, morphology, energy metabolism, DNA damage, and fertility of sperm frozen in Steridyl were evaluated. As a result, Steridyl was shown to be a good extender for equine semen freezing. ABSTRACT: The fertilizing ability of stallion sperm after freezing is lower than in other species. The search for the optimal extender, combination of extenders, and the freezing protocol is relevant. The aim of this study was to compare lactose-chelate-citrate-yolk (LCCY) extender, usually used in Russia, and Steridyl(®) (Minitube) for freezing sperm of stallions. Steridyl is a concentrated extender medium for freezing ruminant semen. It already contains sterilized egg yolk. Semen was collected from nine stallions, aged from 7 to 12 years old. The total and progressive motility of sperm frozen in Steridyl was significantly higher than in semen frozen in LCCY. The number of spermatozoa with normal morphology in samples frozen in LCCY was 60.4 ± 1.72%, and with Steridyl, 72.4 ± 2.10% (p < 0.01). Semen frozen in Steridyl showed good stimulation of respiration by 2.4-DNP, which indicates that oxidative phosphorylation was retained after freezing–thawing. No differences among the extenders were seen with the DNA integrity of spermatozoa. Six out of ten (60%) mares were pregnant after artificial insemination (AI) by LCCY frozen semen, and 9/12 (75%) by Steridyl frozen semen. No differences among extenders were seen in pregnancy rate. In conclusion, Steridyl was proven to be a good diluent for freezing stallion semen, even though it was developed for ruminants. |
format | Online Article Text |
id | pubmed-7601834 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76018342020-11-01 Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions Nikitkina, Elena Musidray, Artem Krutikova, Anna Anipchenko, Polina Plemyashov, Kirill Shiryaev, Gennadiy Animals (Basel) Article SIMPLE SUMMARY: The cryopreservation and long-term storage of semen is one of the methods for accelerated improvement of the genetic qualities of animals. However, horse breeders prefer to use fresh or chilled semen, as the fertilizing capacity of frozen equine semen is much lower. It is important to find extenders, or a combination of extenders, that will improve semen survival after freezing. It is also important that the extender can be easily and simply prepared for use. Steridyl is a concentrate to which you just need to add sterilized water. This extender was developed for ruminants. In this study we tested Steridyl for freezing stallion semen. The motility, morphology, energy metabolism, DNA damage, and fertility of sperm frozen in Steridyl were evaluated. As a result, Steridyl was shown to be a good extender for equine semen freezing. ABSTRACT: The fertilizing ability of stallion sperm after freezing is lower than in other species. The search for the optimal extender, combination of extenders, and the freezing protocol is relevant. The aim of this study was to compare lactose-chelate-citrate-yolk (LCCY) extender, usually used in Russia, and Steridyl(®) (Minitube) for freezing sperm of stallions. Steridyl is a concentrated extender medium for freezing ruminant semen. It already contains sterilized egg yolk. Semen was collected from nine stallions, aged from 7 to 12 years old. The total and progressive motility of sperm frozen in Steridyl was significantly higher than in semen frozen in LCCY. The number of spermatozoa with normal morphology in samples frozen in LCCY was 60.4 ± 1.72%, and with Steridyl, 72.4 ± 2.10% (p < 0.01). Semen frozen in Steridyl showed good stimulation of respiration by 2.4-DNP, which indicates that oxidative phosphorylation was retained after freezing–thawing. No differences among the extenders were seen with the DNA integrity of spermatozoa. Six out of ten (60%) mares were pregnant after artificial insemination (AI) by LCCY frozen semen, and 9/12 (75%) by Steridyl frozen semen. No differences among extenders were seen in pregnancy rate. In conclusion, Steridyl was proven to be a good diluent for freezing stallion semen, even though it was developed for ruminants. MDPI 2020-10-04 /pmc/articles/PMC7601834/ /pubmed/33020383 http://dx.doi.org/10.3390/ani10101801 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nikitkina, Elena Musidray, Artem Krutikova, Anna Anipchenko, Polina Plemyashov, Kirill Shiryaev, Gennadiy Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions |
title | Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions |
title_full | Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions |
title_fullStr | Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions |
title_full_unstemmed | Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions |
title_short | Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions |
title_sort | efficiency of tris-based extender steridyl for semen cryopreservation in stallions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601834/ https://www.ncbi.nlm.nih.gov/pubmed/33020383 http://dx.doi.org/10.3390/ani10101801 |
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