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Epstein-Barr Virus and Human Adenovirus Viremia in Renal Tumors Is Associated with Histological Features of Malignancy

Background: There is growing evidence that viral infections may impact the risk and clinical course of malignancies, including solid tumors. The aim of this study was to assess the possible association of selected chronic/latent viral infections with the clinical course of renal cell carcinoma (RCC)...

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Autores principales: Kryst, Piotr, Poletajew, Sławomir, Wyczałkowska-Tomasik, Aleksandra, Gonczar, Stefan, Wysocki, Maciej, Kapuścińska, Renata, Krajewski, Wojciech, Zgliczyński, Wojciech, Pączek, Leszek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601937/
https://www.ncbi.nlm.nih.gov/pubmed/33023077
http://dx.doi.org/10.3390/jcm9103195
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author Kryst, Piotr
Poletajew, Sławomir
Wyczałkowska-Tomasik, Aleksandra
Gonczar, Stefan
Wysocki, Maciej
Kapuścińska, Renata
Krajewski, Wojciech
Zgliczyński, Wojciech
Pączek, Leszek
author_facet Kryst, Piotr
Poletajew, Sławomir
Wyczałkowska-Tomasik, Aleksandra
Gonczar, Stefan
Wysocki, Maciej
Kapuścińska, Renata
Krajewski, Wojciech
Zgliczyński, Wojciech
Pączek, Leszek
author_sort Kryst, Piotr
collection PubMed
description Background: There is growing evidence that viral infections may impact the risk and clinical course of malignancies, including solid tumors. The aim of this study was to assess the possible association of selected chronic/latent viral infections with the clinical course of renal cell carcinoma (RCC). Methods: In this prospective study we enrolled 27 patients undergoing partial or radical nephrectomy due to the histologically confirmed RCC and followed them up for one year post-operation. Isolation of the nucleic acids was performed using the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany) from tumor tissue and using the EZ1 Virus Mini Kit v2.0 from plasma. The number of viral copies of human adenovirus (ADV), herpes simplex virus HSV-1 and HSV-2, Epstein-Barr virus (EBV), cytomegalovirus (CMV), BK virus (BKV) and John Cunningham virus (JCV) in the tissue and plasma was assessed with real-time PCR. Results: Viral infections were diagnosed in ten patients (37.0%), including three ADV cases (11.1%) and eight EBV cases (29.6%). Infected patients tended to be significantly older (71.3 vs. 57.6 years, p < 0.05), more commonly presented with chronic renal disease (OR 2.4, p < 0.05), diabetes (OR 4.2, p < 0.05) and overweight (OR 2.0, p < 0.05). Regarding oncological data, infected patients were found to have a higher rate of high-grade cancers (OR 5.0, p < 0.05) and a higher rate of papillary RCCs (OR 8.3, p < 0.05). Status of viral infections had no influence on the clinical cancer stage, surgical procedure or survival. Conclusions: EBV and ADV infections are common in renal cancer patients and increase the risk of high-grade RCC presence. While there is no significant impact on short term survival, further studies are needed to assess the relevance of these findings in a long run.
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spelling pubmed-76019372020-11-01 Epstein-Barr Virus and Human Adenovirus Viremia in Renal Tumors Is Associated with Histological Features of Malignancy Kryst, Piotr Poletajew, Sławomir Wyczałkowska-Tomasik, Aleksandra Gonczar, Stefan Wysocki, Maciej Kapuścińska, Renata Krajewski, Wojciech Zgliczyński, Wojciech Pączek, Leszek J Clin Med Article Background: There is growing evidence that viral infections may impact the risk and clinical course of malignancies, including solid tumors. The aim of this study was to assess the possible association of selected chronic/latent viral infections with the clinical course of renal cell carcinoma (RCC). Methods: In this prospective study we enrolled 27 patients undergoing partial or radical nephrectomy due to the histologically confirmed RCC and followed them up for one year post-operation. Isolation of the nucleic acids was performed using the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany) from tumor tissue and using the EZ1 Virus Mini Kit v2.0 from plasma. The number of viral copies of human adenovirus (ADV), herpes simplex virus HSV-1 and HSV-2, Epstein-Barr virus (EBV), cytomegalovirus (CMV), BK virus (BKV) and John Cunningham virus (JCV) in the tissue and plasma was assessed with real-time PCR. Results: Viral infections were diagnosed in ten patients (37.0%), including three ADV cases (11.1%) and eight EBV cases (29.6%). Infected patients tended to be significantly older (71.3 vs. 57.6 years, p < 0.05), more commonly presented with chronic renal disease (OR 2.4, p < 0.05), diabetes (OR 4.2, p < 0.05) and overweight (OR 2.0, p < 0.05). Regarding oncological data, infected patients were found to have a higher rate of high-grade cancers (OR 5.0, p < 0.05) and a higher rate of papillary RCCs (OR 8.3, p < 0.05). Status of viral infections had no influence on the clinical cancer stage, surgical procedure or survival. Conclusions: EBV and ADV infections are common in renal cancer patients and increase the risk of high-grade RCC presence. While there is no significant impact on short term survival, further studies are needed to assess the relevance of these findings in a long run. MDPI 2020-10-02 /pmc/articles/PMC7601937/ /pubmed/33023077 http://dx.doi.org/10.3390/jcm9103195 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kryst, Piotr
Poletajew, Sławomir
Wyczałkowska-Tomasik, Aleksandra
Gonczar, Stefan
Wysocki, Maciej
Kapuścińska, Renata
Krajewski, Wojciech
Zgliczyński, Wojciech
Pączek, Leszek
Epstein-Barr Virus and Human Adenovirus Viremia in Renal Tumors Is Associated with Histological Features of Malignancy
title Epstein-Barr Virus and Human Adenovirus Viremia in Renal Tumors Is Associated with Histological Features of Malignancy
title_full Epstein-Barr Virus and Human Adenovirus Viremia in Renal Tumors Is Associated with Histological Features of Malignancy
title_fullStr Epstein-Barr Virus and Human Adenovirus Viremia in Renal Tumors Is Associated with Histological Features of Malignancy
title_full_unstemmed Epstein-Barr Virus and Human Adenovirus Viremia in Renal Tumors Is Associated with Histological Features of Malignancy
title_short Epstein-Barr Virus and Human Adenovirus Viremia in Renal Tumors Is Associated with Histological Features of Malignancy
title_sort epstein-barr virus and human adenovirus viremia in renal tumors is associated with histological features of malignancy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601937/
https://www.ncbi.nlm.nih.gov/pubmed/33023077
http://dx.doi.org/10.3390/jcm9103195
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