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LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

Investigations were carried out to study the effects of light-emitting diode (LED) lights on growth and development of isosteroidal alkaloids in embryogenic calli of Fritillaria cirrhosa D. Don, an important traditional Chinese medicine herb. Calli were cultured in glass bottles, each containing 100...

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Autores principales: Chen, Chia-Chen, Lee, Maw-Rong, Wu, Chi-Rei, Ke, Hsin-Ju, Xie, Hui-Min, Tsay, Hsin-Sheng, Agrawal, Dinesh Chandra, Chang, Hung-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602057/
https://www.ncbi.nlm.nih.gov/pubmed/33066243
http://dx.doi.org/10.3390/plants9101351
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author Chen, Chia-Chen
Lee, Maw-Rong
Wu, Chi-Rei
Ke, Hsin-Ju
Xie, Hui-Min
Tsay, Hsin-Sheng
Agrawal, Dinesh Chandra
Chang, Hung-Chi
author_facet Chen, Chia-Chen
Lee, Maw-Rong
Wu, Chi-Rei
Ke, Hsin-Ju
Xie, Hui-Min
Tsay, Hsin-Sheng
Agrawal, Dinesh Chandra
Chang, Hung-Chi
author_sort Chen, Chia-Chen
collection PubMed
description Investigations were carried out to study the effects of light-emitting diode (LED) lights on growth and development of isosteroidal alkaloids in embryogenic calli of Fritillaria cirrhosa D. Don, an important traditional Chinese medicine herb. Calli were cultured in glass bottles, each containing 100 mL of Murashige and Skoog’s basal medium supplemented with 2% sucrose and 0.4% gellan gum powder, a gelling agent. These bottles were incubated in a specially designed plant growth chamber equipped with eight different LED lights consisting of single or combinations of four different light spectra emitting blue (450 nm), green (525 nm), red (660 nm), and far-red (730 nm) light. After three months of incubation, morphological changes in embryogenic calli were recorded, and LC-MS/MS analysis of cultures was carried out for peimisine, sipeimine, peiminine, and peimine. The highest number of somatic embryos and the maximum fresh weight was recorded in calli incubated under red (9R), infrared (9IR), and a combination of red+blue+infrared (3R3B3IR), respectively, in decreasing order. The highest contents of peimisine, peiminine, and peimine were recorded under red (9R) and infrared (9IR) lights, respectively. Eight LED lights had significant effects on the morphogenesis of embryogenic calli of F. cirrhosa D. Don and contents of isosteroidal alkaloids.
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spelling pubmed-76020572020-11-01 LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb Chen, Chia-Chen Lee, Maw-Rong Wu, Chi-Rei Ke, Hsin-Ju Xie, Hui-Min Tsay, Hsin-Sheng Agrawal, Dinesh Chandra Chang, Hung-Chi Plants (Basel) Article Investigations were carried out to study the effects of light-emitting diode (LED) lights on growth and development of isosteroidal alkaloids in embryogenic calli of Fritillaria cirrhosa D. Don, an important traditional Chinese medicine herb. Calli were cultured in glass bottles, each containing 100 mL of Murashige and Skoog’s basal medium supplemented with 2% sucrose and 0.4% gellan gum powder, a gelling agent. These bottles were incubated in a specially designed plant growth chamber equipped with eight different LED lights consisting of single or combinations of four different light spectra emitting blue (450 nm), green (525 nm), red (660 nm), and far-red (730 nm) light. After three months of incubation, morphological changes in embryogenic calli were recorded, and LC-MS/MS analysis of cultures was carried out for peimisine, sipeimine, peiminine, and peimine. The highest number of somatic embryos and the maximum fresh weight was recorded in calli incubated under red (9R), infrared (9IR), and a combination of red+blue+infrared (3R3B3IR), respectively, in decreasing order. The highest contents of peimisine, peiminine, and peimine were recorded under red (9R) and infrared (9IR) lights, respectively. Eight LED lights had significant effects on the morphogenesis of embryogenic calli of F. cirrhosa D. Don and contents of isosteroidal alkaloids. MDPI 2020-10-13 /pmc/articles/PMC7602057/ /pubmed/33066243 http://dx.doi.org/10.3390/plants9101351 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Chia-Chen
Lee, Maw-Rong
Wu, Chi-Rei
Ke, Hsin-Ju
Xie, Hui-Min
Tsay, Hsin-Sheng
Agrawal, Dinesh Chandra
Chang, Hung-Chi
LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb
title LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb
title_full LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb
title_fullStr LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb
title_full_unstemmed LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb
title_short LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb
title_sort led lights affecting morphogenesis and isosteroidal alkaloid contents in fritillaria cirrhosa d. don—an important chinese medicinal herb
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602057/
https://www.ncbi.nlm.nih.gov/pubmed/33066243
http://dx.doi.org/10.3390/plants9101351
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