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Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities

The composition of essential oils of Chrysanthemum indicum and C. morifolium were comparatively studied using both Gas Chromatography/Flame ionization Detector (GC/FID) and Gas Chromatography/Mass spectrometry (GC/MS) analyses. The antiviral activity was determined using a plaque reduction assay aga...

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Autores principales: Youssef, Fadia S., Eid, Safaa Y., Alshammari, Elham, Ashour, Mohamed L., Wink, Michael, El-Readi, Mahmoud Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602131/
https://www.ncbi.nlm.nih.gov/pubmed/33066507
http://dx.doi.org/10.3390/foods9101460
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author Youssef, Fadia S.
Eid, Safaa Y.
Alshammari, Elham
Ashour, Mohamed L.
Wink, Michael
El-Readi, Mahmoud Z.
author_facet Youssef, Fadia S.
Eid, Safaa Y.
Alshammari, Elham
Ashour, Mohamed L.
Wink, Michael
El-Readi, Mahmoud Z.
author_sort Youssef, Fadia S.
collection PubMed
description The composition of essential oils of Chrysanthemum indicum and C. morifolium were comparatively studied using both Gas Chromatography/Flame ionization Detector (GC/FID) and Gas Chromatography/Mass spectrometry (GC/MS) analyses. The antiviral activity was determined using a plaque reduction assay against three common viruses namely, herpes simplex type-1 (HSV-1), hepatitis A (HAV) and vesicular stomatitis virus (VSV). The antimicrobial activity was assessed using agar diffusion and microdilution methods and the minimum inhibitory concentration (MIC) values were determined. In addition, the anti-mycobacterial evaluation was carried out using the Alamar blue assay and the effect against Helicobacter pylori was investigated. The anti-trypanosomal activity was evaluated using the resazurin method. GC investigations revealed that camphor is the major constituent of both oils accounting for 36.69 and 14.56% in the essential oils from C. indicum and C. morifolium, respectively. C. indicum was biologically more active in all experiments; it exhibited a notable antitrypanosomal activity with an IC(50) value equals 45.89 μg/mL and a notable antimicrobial activity versus Streptococcus agalactiae with a MIC value of 62.5 μg/mL. It also inhibited the replication of VSV with an IC(50) value of 3.14 μg/mL. Both oils revealed antioxidant potential with IC(50) values of 2.21 and 2.59 mg/mL for C. indicum and C. morifolium, respectively. This study provides evidence beyond the traditional use of both Chrysanthemum indicum and C. morifolium as anti-infective agents. Thus they could be used as spices in food and can be incorporated in different food products and pharmaceutical preparations as natural preservatives possessing antioxidant potential.
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spelling pubmed-76021312020-11-01 Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities Youssef, Fadia S. Eid, Safaa Y. Alshammari, Elham Ashour, Mohamed L. Wink, Michael El-Readi, Mahmoud Z. Foods Article The composition of essential oils of Chrysanthemum indicum and C. morifolium were comparatively studied using both Gas Chromatography/Flame ionization Detector (GC/FID) and Gas Chromatography/Mass spectrometry (GC/MS) analyses. The antiviral activity was determined using a plaque reduction assay against three common viruses namely, herpes simplex type-1 (HSV-1), hepatitis A (HAV) and vesicular stomatitis virus (VSV). The antimicrobial activity was assessed using agar diffusion and microdilution methods and the minimum inhibitory concentration (MIC) values were determined. In addition, the anti-mycobacterial evaluation was carried out using the Alamar blue assay and the effect against Helicobacter pylori was investigated. The anti-trypanosomal activity was evaluated using the resazurin method. GC investigations revealed that camphor is the major constituent of both oils accounting for 36.69 and 14.56% in the essential oils from C. indicum and C. morifolium, respectively. C. indicum was biologically more active in all experiments; it exhibited a notable antitrypanosomal activity with an IC(50) value equals 45.89 μg/mL and a notable antimicrobial activity versus Streptococcus agalactiae with a MIC value of 62.5 μg/mL. It also inhibited the replication of VSV with an IC(50) value of 3.14 μg/mL. Both oils revealed antioxidant potential with IC(50) values of 2.21 and 2.59 mg/mL for C. indicum and C. morifolium, respectively. This study provides evidence beyond the traditional use of both Chrysanthemum indicum and C. morifolium as anti-infective agents. Thus they could be used as spices in food and can be incorporated in different food products and pharmaceutical preparations as natural preservatives possessing antioxidant potential. MDPI 2020-10-14 /pmc/articles/PMC7602131/ /pubmed/33066507 http://dx.doi.org/10.3390/foods9101460 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Youssef, Fadia S.
Eid, Safaa Y.
Alshammari, Elham
Ashour, Mohamed L.
Wink, Michael
El-Readi, Mahmoud Z.
Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities
title Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities
title_full Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities
title_fullStr Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities
title_full_unstemmed Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities
title_short Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities
title_sort chrysanthemum indicum and chrysanthemum morifolium: chemical composition of their essential oils and their potential use as natural preservatives with antimicrobial and antioxidant activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602131/
https://www.ncbi.nlm.nih.gov/pubmed/33066507
http://dx.doi.org/10.3390/foods9101460
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