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Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Drought stress limits plant growth and productivity. It triggers many responses by inducing changes in plant morphology and physiology. KDML105 rice is a key rice variety in Thailand and is normally grown in the northeastern part of the country. The chromosome segment substitution lines (CSSLs) were...

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Autores principales: Punchkhon, Chutarat, Plaimas, Kitiporn, Buaboocha, Teerapong, Siangliw, Jonaliza L., Toojinda, Theerayut, Comai, Luca, De Diego, Nuria, Spíchal, Lukáš, Chadchawan, Supachitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602393/
https://www.ncbi.nlm.nih.gov/pubmed/33066648
http://dx.doi.org/10.3390/genes11101197
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author Punchkhon, Chutarat
Plaimas, Kitiporn
Buaboocha, Teerapong
Siangliw, Jonaliza L.
Toojinda, Theerayut
Comai, Luca
De Diego, Nuria
Spíchal, Lukáš
Chadchawan, Supachitra
author_facet Punchkhon, Chutarat
Plaimas, Kitiporn
Buaboocha, Teerapong
Siangliw, Jonaliza L.
Toojinda, Theerayut
Comai, Luca
De Diego, Nuria
Spíchal, Lukáš
Chadchawan, Supachitra
author_sort Punchkhon, Chutarat
collection PubMed
description Drought stress limits plant growth and productivity. It triggers many responses by inducing changes in plant morphology and physiology. KDML105 rice is a key rice variety in Thailand and is normally grown in the northeastern part of the country. The chromosome segment substitution lines (CSSLs) were developed by transferring putative drought tolerance loci (QTLs) on chromosome 1, 3, 4, 8, or 9 into the KDML105 rice genome. CSSL104 is a drought-tolerant line with higher net photosynthesis and leaf water potential than KDML105 rice. The analysis of CSSL104 gene regulation identified the loci associated with these traits via gene co-expression network analysis. Most of the predicted genes are involved in the photosynthesis process. These genes are also conserved in Arabidopsis thaliana. Seven genes encoding chloroplast proteins were selected for further analysis through characterization of Arabidopsis tagged mutants. The response of these mutants to drought stress was analyzed daily for seven days after treatment by scoring green tissue areas via the PlantScreen™ XYZ system. Mutation of these genes affected green areas of the plant and stability index under drought stress, suggesting their involvement in drought tolerance.
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spelling pubmed-76023932020-11-01 Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice Punchkhon, Chutarat Plaimas, Kitiporn Buaboocha, Teerapong Siangliw, Jonaliza L. Toojinda, Theerayut Comai, Luca De Diego, Nuria Spíchal, Lukáš Chadchawan, Supachitra Genes (Basel) Article Drought stress limits plant growth and productivity. It triggers many responses by inducing changes in plant morphology and physiology. KDML105 rice is a key rice variety in Thailand and is normally grown in the northeastern part of the country. The chromosome segment substitution lines (CSSLs) were developed by transferring putative drought tolerance loci (QTLs) on chromosome 1, 3, 4, 8, or 9 into the KDML105 rice genome. CSSL104 is a drought-tolerant line with higher net photosynthesis and leaf water potential than KDML105 rice. The analysis of CSSL104 gene regulation identified the loci associated with these traits via gene co-expression network analysis. Most of the predicted genes are involved in the photosynthesis process. These genes are also conserved in Arabidopsis thaliana. Seven genes encoding chloroplast proteins were selected for further analysis through characterization of Arabidopsis tagged mutants. The response of these mutants to drought stress was analyzed daily for seven days after treatment by scoring green tissue areas via the PlantScreen™ XYZ system. Mutation of these genes affected green areas of the plant and stability index under drought stress, suggesting their involvement in drought tolerance. MDPI 2020-10-14 /pmc/articles/PMC7602393/ /pubmed/33066648 http://dx.doi.org/10.3390/genes11101197 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Punchkhon, Chutarat
Plaimas, Kitiporn
Buaboocha, Teerapong
Siangliw, Jonaliza L.
Toojinda, Theerayut
Comai, Luca
De Diego, Nuria
Spíchal, Lukáš
Chadchawan, Supachitra
Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice
title Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice
title_full Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice
title_fullStr Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice
title_full_unstemmed Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice
title_short Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice
title_sort drought-tolerance gene identification using genome comparison and co-expression network analysis of chromosome substitution lines in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602393/
https://www.ncbi.nlm.nih.gov/pubmed/33066648
http://dx.doi.org/10.3390/genes11101197
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