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SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study

The course of SARS-CoV-2 infection ranges from asymptomatic to a multiorgan disease. In this observational study, we investigated SARS-CoV-2 infected subjects with defined outcomes, evaluating the relationship between viral load and single nucleotide polymorphisms of genes codifying for IFNλs (inter...

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Autores principales: Amodio, Emanuele, Pipitone, Rosaria Maria, Grimaudo, Stefania, Immordino, Palmira, Maida, Carmelo Massimo, Prestileo, Tullio, Restivo, Vincenzo, Tramuto, Fabio, Vitale, Francesco, Craxì, Antonio, Casuccio, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602550/
https://www.ncbi.nlm.nih.gov/pubmed/33076493
http://dx.doi.org/10.3390/jcm9103315
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author Amodio, Emanuele
Pipitone, Rosaria Maria
Grimaudo, Stefania
Immordino, Palmira
Maida, Carmelo Massimo
Prestileo, Tullio
Restivo, Vincenzo
Tramuto, Fabio
Vitale, Francesco
Craxì, Antonio
Casuccio, Alessandra
author_facet Amodio, Emanuele
Pipitone, Rosaria Maria
Grimaudo, Stefania
Immordino, Palmira
Maida, Carmelo Massimo
Prestileo, Tullio
Restivo, Vincenzo
Tramuto, Fabio
Vitale, Francesco
Craxì, Antonio
Casuccio, Alessandra
author_sort Amodio, Emanuele
collection PubMed
description The course of SARS-CoV-2 infection ranges from asymptomatic to a multiorgan disease. In this observational study, we investigated SARS-CoV-2 infected subjects with defined outcomes, evaluating the relationship between viral load and single nucleotide polymorphisms of genes codifying for IFNλs (interferon). The study enrolled 381 patients with laboratory-confirmed SARS-CoV-2 infection. For each patient, a standardized form was filled including sociodemographic variables and clinical outcomes. The host’s gene polymorphisms (IFNL3 rs1297860 C/T and INFL4 rs368234815 TT/ΔG) and RtReal-Time PCR cycle threshold (PCR Ct) value on SARS-CoV-2 were assessed on nasal, pharyngeal or nasopharyngeal swabs. Higher viral loads were found in patients aged > 74 years and homozygous mutant polymorphisms DG in IFNL4 (adj-OR = 1.16, 95% CI = 1.01–1.34 and adj-OR = 1.24, 95% CI = 1.09–1.40, respectively). After adjusting for age and sex, a statistically significantly lower risk of hospitalization was observed in subjects with higher RtReal-Time PCR cycle threshold values (adj-OR = 0.95, 95% CI = 0.91, 0.99; p = 0.028). Our data support the correlation between SARS-CoV-2 load and disease severity, and suggest that IFNλ polymorphisms could affect the ability of the host to modulate viral infection without a clear impact on the outcome of COVID-19.
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spelling pubmed-76025502020-11-01 SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study Amodio, Emanuele Pipitone, Rosaria Maria Grimaudo, Stefania Immordino, Palmira Maida, Carmelo Massimo Prestileo, Tullio Restivo, Vincenzo Tramuto, Fabio Vitale, Francesco Craxì, Antonio Casuccio, Alessandra J Clin Med Article The course of SARS-CoV-2 infection ranges from asymptomatic to a multiorgan disease. In this observational study, we investigated SARS-CoV-2 infected subjects with defined outcomes, evaluating the relationship between viral load and single nucleotide polymorphisms of genes codifying for IFNλs (interferon). The study enrolled 381 patients with laboratory-confirmed SARS-CoV-2 infection. For each patient, a standardized form was filled including sociodemographic variables and clinical outcomes. The host’s gene polymorphisms (IFNL3 rs1297860 C/T and INFL4 rs368234815 TT/ΔG) and RtReal-Time PCR cycle threshold (PCR Ct) value on SARS-CoV-2 were assessed on nasal, pharyngeal or nasopharyngeal swabs. Higher viral loads were found in patients aged > 74 years and homozygous mutant polymorphisms DG in IFNL4 (adj-OR = 1.16, 95% CI = 1.01–1.34 and adj-OR = 1.24, 95% CI = 1.09–1.40, respectively). After adjusting for age and sex, a statistically significantly lower risk of hospitalization was observed in subjects with higher RtReal-Time PCR cycle threshold values (adj-OR = 0.95, 95% CI = 0.91, 0.99; p = 0.028). Our data support the correlation between SARS-CoV-2 load and disease severity, and suggest that IFNλ polymorphisms could affect the ability of the host to modulate viral infection without a clear impact on the outcome of COVID-19. MDPI 2020-10-15 /pmc/articles/PMC7602550/ /pubmed/33076493 http://dx.doi.org/10.3390/jcm9103315 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amodio, Emanuele
Pipitone, Rosaria Maria
Grimaudo, Stefania
Immordino, Palmira
Maida, Carmelo Massimo
Prestileo, Tullio
Restivo, Vincenzo
Tramuto, Fabio
Vitale, Francesco
Craxì, Antonio
Casuccio, Alessandra
SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study
title SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study
title_full SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study
title_fullStr SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study
title_full_unstemmed SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study
title_short SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study
title_sort sars-cov-2 viral load, ifnλ polymorphisms and the course of covid-19: an observational study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602550/
https://www.ncbi.nlm.nih.gov/pubmed/33076493
http://dx.doi.org/10.3390/jcm9103315
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