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Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment
Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602568/ https://www.ncbi.nlm.nih.gov/pubmed/33076472 http://dx.doi.org/10.3390/ijerph17207514 |
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author | Matsuda, Shuhei Yamato, Takahiro Mochizuki, Yoshiyuki Sekiguchi, Yoshinori Ohtsuki, Takashi |
author_facet | Matsuda, Shuhei Yamato, Takahiro Mochizuki, Yoshiyuki Sekiguchi, Yoshinori Ohtsuki, Takashi |
author_sort | Matsuda, Shuhei |
collection | PubMed |
description | Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrate loading often become problematic. This study investigated changes in microbial communities in the overacidification state of the thermophilic methane fermentation process with beverage waste by establishing a simulated batch culture. We assessed 20 mL-scale batch cultures using a simulant beverage waste mixture (SBWM) with different amounts of addition; high cumulative methane production was achieved by adding 5 mL of SBWM (11358 mg—chemical oxygen demand—COD/L of organic loading), and overacidification was observed by adding 10 mL of SBWM (22715 mg—COD/L of organic loading). The results of 16S rRNA amplicon sequence analysis using nanopore sequencer suggested that Coprothermobacter proteolyticus, Defluviitoga tunisiensis, Acetomicrobium mobile, and Thermosediminibacter oceani were predominantly involved in hydrolysis/acidogenesis/acetogenesis processes, whereas Methanothrix soehngenii was the major acetotrophic methane producer. A comparison of microbial population between the methane-producing cultures and overacidification cultures revealed characteristic population changes especially in some minor species under 0.2% of population. We concluded that careful monitoring of population changes of the minor species is a potential indicator for prediction of overacidification. |
format | Online Article Text |
id | pubmed-7602568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76025682020-11-01 Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment Matsuda, Shuhei Yamato, Takahiro Mochizuki, Yoshiyuki Sekiguchi, Yoshinori Ohtsuki, Takashi Int J Environ Res Public Health Article Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrate loading often become problematic. This study investigated changes in microbial communities in the overacidification state of the thermophilic methane fermentation process with beverage waste by establishing a simulated batch culture. We assessed 20 mL-scale batch cultures using a simulant beverage waste mixture (SBWM) with different amounts of addition; high cumulative methane production was achieved by adding 5 mL of SBWM (11358 mg—chemical oxygen demand—COD/L of organic loading), and overacidification was observed by adding 10 mL of SBWM (22715 mg—COD/L of organic loading). The results of 16S rRNA amplicon sequence analysis using nanopore sequencer suggested that Coprothermobacter proteolyticus, Defluviitoga tunisiensis, Acetomicrobium mobile, and Thermosediminibacter oceani were predominantly involved in hydrolysis/acidogenesis/acetogenesis processes, whereas Methanothrix soehngenii was the major acetotrophic methane producer. A comparison of microbial population between the methane-producing cultures and overacidification cultures revealed characteristic population changes especially in some minor species under 0.2% of population. We concluded that careful monitoring of population changes of the minor species is a potential indicator for prediction of overacidification. MDPI 2020-10-15 2020-10 /pmc/articles/PMC7602568/ /pubmed/33076472 http://dx.doi.org/10.3390/ijerph17207514 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Matsuda, Shuhei Yamato, Takahiro Mochizuki, Yoshiyuki Sekiguchi, Yoshinori Ohtsuki, Takashi Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment |
title | Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment |
title_full | Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment |
title_fullStr | Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment |
title_full_unstemmed | Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment |
title_short | Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment |
title_sort | batch-mode analysis of thermophilic methanogenic microbial community changes in the overacidification stage in beverage waste treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602568/ https://www.ncbi.nlm.nih.gov/pubmed/33076472 http://dx.doi.org/10.3390/ijerph17207514 |
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