Cargando…

Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment

Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrat...

Descripción completa

Detalles Bibliográficos
Autores principales: Matsuda, Shuhei, Yamato, Takahiro, Mochizuki, Yoshiyuki, Sekiguchi, Yoshinori, Ohtsuki, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602568/
https://www.ncbi.nlm.nih.gov/pubmed/33076472
http://dx.doi.org/10.3390/ijerph17207514
_version_ 1783603712534511616
author Matsuda, Shuhei
Yamato, Takahiro
Mochizuki, Yoshiyuki
Sekiguchi, Yoshinori
Ohtsuki, Takashi
author_facet Matsuda, Shuhei
Yamato, Takahiro
Mochizuki, Yoshiyuki
Sekiguchi, Yoshinori
Ohtsuki, Takashi
author_sort Matsuda, Shuhei
collection PubMed
description Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrate loading often become problematic. This study investigated changes in microbial communities in the overacidification state of the thermophilic methane fermentation process with beverage waste by establishing a simulated batch culture. We assessed 20 mL-scale batch cultures using a simulant beverage waste mixture (SBWM) with different amounts of addition; high cumulative methane production was achieved by adding 5 mL of SBWM (11358 mg—chemical oxygen demand—COD/L of organic loading), and overacidification was observed by adding 10 mL of SBWM (22715 mg—COD/L of organic loading). The results of 16S rRNA amplicon sequence analysis using nanopore sequencer suggested that Coprothermobacter proteolyticus, Defluviitoga tunisiensis, Acetomicrobium mobile, and Thermosediminibacter oceani were predominantly involved in hydrolysis/acidogenesis/acetogenesis processes, whereas Methanothrix soehngenii was the major acetotrophic methane producer. A comparison of microbial population between the methane-producing cultures and overacidification cultures revealed characteristic population changes especially in some minor species under 0.2% of population. We concluded that careful monitoring of population changes of the minor species is a potential indicator for prediction of overacidification.
format Online
Article
Text
id pubmed-7602568
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76025682020-11-01 Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment Matsuda, Shuhei Yamato, Takahiro Mochizuki, Yoshiyuki Sekiguchi, Yoshinori Ohtsuki, Takashi Int J Environ Res Public Health Article Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrate loading often become problematic. This study investigated changes in microbial communities in the overacidification state of the thermophilic methane fermentation process with beverage waste by establishing a simulated batch culture. We assessed 20 mL-scale batch cultures using a simulant beverage waste mixture (SBWM) with different amounts of addition; high cumulative methane production was achieved by adding 5 mL of SBWM (11358 mg—chemical oxygen demand—COD/L of organic loading), and overacidification was observed by adding 10 mL of SBWM (22715 mg—COD/L of organic loading). The results of 16S rRNA amplicon sequence analysis using nanopore sequencer suggested that Coprothermobacter proteolyticus, Defluviitoga tunisiensis, Acetomicrobium mobile, and Thermosediminibacter oceani were predominantly involved in hydrolysis/acidogenesis/acetogenesis processes, whereas Methanothrix soehngenii was the major acetotrophic methane producer. A comparison of microbial population between the methane-producing cultures and overacidification cultures revealed characteristic population changes especially in some minor species under 0.2% of population. We concluded that careful monitoring of population changes of the minor species is a potential indicator for prediction of overacidification. MDPI 2020-10-15 2020-10 /pmc/articles/PMC7602568/ /pubmed/33076472 http://dx.doi.org/10.3390/ijerph17207514 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matsuda, Shuhei
Yamato, Takahiro
Mochizuki, Yoshiyuki
Sekiguchi, Yoshinori
Ohtsuki, Takashi
Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment
title Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment
title_full Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment
title_fullStr Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment
title_full_unstemmed Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment
title_short Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment
title_sort batch-mode analysis of thermophilic methanogenic microbial community changes in the overacidification stage in beverage waste treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602568/
https://www.ncbi.nlm.nih.gov/pubmed/33076472
http://dx.doi.org/10.3390/ijerph17207514
work_keys_str_mv AT matsudashuhei batchmodeanalysisofthermophilicmethanogenicmicrobialcommunitychangesintheoveracidificationstageinbeveragewastetreatment
AT yamatotakahiro batchmodeanalysisofthermophilicmethanogenicmicrobialcommunitychangesintheoveracidificationstageinbeveragewastetreatment
AT mochizukiyoshiyuki batchmodeanalysisofthermophilicmethanogenicmicrobialcommunitychangesintheoveracidificationstageinbeveragewastetreatment
AT sekiguchiyoshinori batchmodeanalysisofthermophilicmethanogenicmicrobialcommunitychangesintheoveracidificationstageinbeveragewastetreatment
AT ohtsukitakashi batchmodeanalysisofthermophilicmethanogenicmicrobialcommunitychangesintheoveracidificationstageinbeveragewastetreatment