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Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa
Emerging pests and diseases are a major threat to food production worldwide. In a recent survey, Tomato torrado virus (ToTV) was identified on tomato crops in the Limpopo province of South Africa and a first report of the disease was published. In this follow-up study, the full genome sequence of a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602605/ https://www.ncbi.nlm.nih.gov/pubmed/33076368 http://dx.doi.org/10.3390/v12101167 |
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author | Moodley, Vaneson Gubba, Augustine Mafongoya, Paramu L. |
author_facet | Moodley, Vaneson Gubba, Augustine Mafongoya, Paramu L. |
author_sort | Moodley, Vaneson |
collection | PubMed |
description | Emerging pests and diseases are a major threat to food production worldwide. In a recent survey, Tomato torrado virus (ToTV) was identified on tomato crops in the Limpopo province of South Africa and a first report of the disease was published. In this follow-up study, the full genome sequence of a tomato-infecting isolate of ToTV from South Africa was elucidated. High-throughput sequencing was used to generate the full genome of ToTV infecting tomato crops in South Africa. The longest contig obtained for the RNA-1 and RNA-2 genome of ToTV was comprised of 7420 and 5381 nucleotides (nt), respectively. Blast analysis of the RNA-1 sequence of ToTV from South Africa (ToT-186) matched 99% to a Spanish and Polish isolate; the RNA-2 segment of ToTV from South Africa (ToT-186) matched 99% to ToTV isolates from Italy and Poland, respectively. The information presented in this study will go a long way towards better understanding the emergence and spread of ToTV and devising sustainable management of ToTV diseases. |
format | Online Article Text |
id | pubmed-7602605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76026052020-11-01 Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa Moodley, Vaneson Gubba, Augustine Mafongoya, Paramu L. Viruses Article Emerging pests and diseases are a major threat to food production worldwide. In a recent survey, Tomato torrado virus (ToTV) was identified on tomato crops in the Limpopo province of South Africa and a first report of the disease was published. In this follow-up study, the full genome sequence of a tomato-infecting isolate of ToTV from South Africa was elucidated. High-throughput sequencing was used to generate the full genome of ToTV infecting tomato crops in South Africa. The longest contig obtained for the RNA-1 and RNA-2 genome of ToTV was comprised of 7420 and 5381 nucleotides (nt), respectively. Blast analysis of the RNA-1 sequence of ToTV from South Africa (ToT-186) matched 99% to a Spanish and Polish isolate; the RNA-2 segment of ToTV from South Africa (ToT-186) matched 99% to ToTV isolates from Italy and Poland, respectively. The information presented in this study will go a long way towards better understanding the emergence and spread of ToTV and devising sustainable management of ToTV diseases. MDPI 2020-10-15 /pmc/articles/PMC7602605/ /pubmed/33076368 http://dx.doi.org/10.3390/v12101167 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moodley, Vaneson Gubba, Augustine Mafongoya, Paramu L. Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa |
title | Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa |
title_full | Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa |
title_fullStr | Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa |
title_full_unstemmed | Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa |
title_short | Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa |
title_sort | emergence and full genome analysis of tomato torrado virus in south africa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602605/ https://www.ncbi.nlm.nih.gov/pubmed/33076368 http://dx.doi.org/10.3390/v12101167 |
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