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Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa

Emerging pests and diseases are a major threat to food production worldwide. In a recent survey, Tomato torrado virus (ToTV) was identified on tomato crops in the Limpopo province of South Africa and a first report of the disease was published. In this follow-up study, the full genome sequence of a...

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Detalles Bibliográficos
Autores principales: Moodley, Vaneson, Gubba, Augustine, Mafongoya, Paramu L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602605/
https://www.ncbi.nlm.nih.gov/pubmed/33076368
http://dx.doi.org/10.3390/v12101167
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author Moodley, Vaneson
Gubba, Augustine
Mafongoya, Paramu L.
author_facet Moodley, Vaneson
Gubba, Augustine
Mafongoya, Paramu L.
author_sort Moodley, Vaneson
collection PubMed
description Emerging pests and diseases are a major threat to food production worldwide. In a recent survey, Tomato torrado virus (ToTV) was identified on tomato crops in the Limpopo province of South Africa and a first report of the disease was published. In this follow-up study, the full genome sequence of a tomato-infecting isolate of ToTV from South Africa was elucidated. High-throughput sequencing was used to generate the full genome of ToTV infecting tomato crops in South Africa. The longest contig obtained for the RNA-1 and RNA-2 genome of ToTV was comprised of 7420 and 5381 nucleotides (nt), respectively. Blast analysis of the RNA-1 sequence of ToTV from South Africa (ToT-186) matched 99% to a Spanish and Polish isolate; the RNA-2 segment of ToTV from South Africa (ToT-186) matched 99% to ToTV isolates from Italy and Poland, respectively. The information presented in this study will go a long way towards better understanding the emergence and spread of ToTV and devising sustainable management of ToTV diseases.
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spelling pubmed-76026052020-11-01 Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa Moodley, Vaneson Gubba, Augustine Mafongoya, Paramu L. Viruses Article Emerging pests and diseases are a major threat to food production worldwide. In a recent survey, Tomato torrado virus (ToTV) was identified on tomato crops in the Limpopo province of South Africa and a first report of the disease was published. In this follow-up study, the full genome sequence of a tomato-infecting isolate of ToTV from South Africa was elucidated. High-throughput sequencing was used to generate the full genome of ToTV infecting tomato crops in South Africa. The longest contig obtained for the RNA-1 and RNA-2 genome of ToTV was comprised of 7420 and 5381 nucleotides (nt), respectively. Blast analysis of the RNA-1 sequence of ToTV from South Africa (ToT-186) matched 99% to a Spanish and Polish isolate; the RNA-2 segment of ToTV from South Africa (ToT-186) matched 99% to ToTV isolates from Italy and Poland, respectively. The information presented in this study will go a long way towards better understanding the emergence and spread of ToTV and devising sustainable management of ToTV diseases. MDPI 2020-10-15 /pmc/articles/PMC7602605/ /pubmed/33076368 http://dx.doi.org/10.3390/v12101167 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moodley, Vaneson
Gubba, Augustine
Mafongoya, Paramu L.
Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa
title Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa
title_full Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa
title_fullStr Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa
title_full_unstemmed Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa
title_short Emergence and Full Genome Analysis of Tomato Torrado Virus in South Africa
title_sort emergence and full genome analysis of tomato torrado virus in south africa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602605/
https://www.ncbi.nlm.nih.gov/pubmed/33076368
http://dx.doi.org/10.3390/v12101167
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