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Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation

SIMPLE SUMMARY: The transcriptomic and metabolomic differences in rice leaves after infestation by the rice leaf folder Cnaphalocrocis medinalis were investigated for better understanding of the mechanisms of rice defenses against this species. The results suggest that C. medinalis infestation can i...

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Autores principales: Wang, Yuqi, Liu, Qingsong, Du, Lixiao, Hallerman, Eric M., Li, Yunhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602657/
https://www.ncbi.nlm.nih.gov/pubmed/33076419
http://dx.doi.org/10.3390/insects11100705
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author Wang, Yuqi
Liu, Qingsong
Du, Lixiao
Hallerman, Eric M.
Li, Yunhe
author_facet Wang, Yuqi
Liu, Qingsong
Du, Lixiao
Hallerman, Eric M.
Li, Yunhe
author_sort Wang, Yuqi
collection PubMed
description SIMPLE SUMMARY: The transcriptomic and metabolomic differences in rice leaves after infestation by the rice leaf folder Cnaphalocrocis medinalis were investigated for better understanding of the mechanisms of rice defenses against this species. The results suggest that C. medinalis infestation can induce rapid and precise defense responses involved in many primary and secondary metabolic processes in rice leaves, and the jasmonic acid (JA)-dependent signaling pathway plays vital roles in the response of rice plants to this pest species. These results provide comprehensive insights into the defense system of rice to the rice leaf folder and may facilitate the development of insect-resistant rice varieties by identifying molecular targets for selection. ABSTRACT: Interactions between plants and insect herbivores are important determinants of plant productivity in cultivated and natural agricultural fields. The rice leaf folder (Cnaphalocrocis medinalis) causes tremendous damage to rice production in Asian countries. However, little information is available about how rice plants defend themselves against this destructive pest at molecular and biochemical levels. Here, we observed the transcriptomic and metabolomic differences in rice leaves after 0, 1, 6, 12, and 24 h of being fed by C. medinalis using RNA sequencing and metabolome profiling. Transcriptional analyses showed that gene expression responds rapidly to leaf folder infestation, with the most significant transcriptional changes occurring within 6 h after the initiation of feeding. Metabolite abundance changed more slowly than gene expression. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that the rice transcriptional response to infestation involved genes encoding protein kinases, transcription factors, biosynthesis of secondary metabolites, photosynthesis, and phytohormone signaling. Moreover, the jasmonic acid-dependent signaling pathway triggered by leaf folder herbivory played a vital role in rice defense against this pest. Taken together, our results provide comprehensive insights into the defense system of rice to this species and may inform the development of insect-resistant rice varieties.
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spelling pubmed-76026572020-11-01 Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation Wang, Yuqi Liu, Qingsong Du, Lixiao Hallerman, Eric M. Li, Yunhe Insects Article SIMPLE SUMMARY: The transcriptomic and metabolomic differences in rice leaves after infestation by the rice leaf folder Cnaphalocrocis medinalis were investigated for better understanding of the mechanisms of rice defenses against this species. The results suggest that C. medinalis infestation can induce rapid and precise defense responses involved in many primary and secondary metabolic processes in rice leaves, and the jasmonic acid (JA)-dependent signaling pathway plays vital roles in the response of rice plants to this pest species. These results provide comprehensive insights into the defense system of rice to the rice leaf folder and may facilitate the development of insect-resistant rice varieties by identifying molecular targets for selection. ABSTRACT: Interactions between plants and insect herbivores are important determinants of plant productivity in cultivated and natural agricultural fields. The rice leaf folder (Cnaphalocrocis medinalis) causes tremendous damage to rice production in Asian countries. However, little information is available about how rice plants defend themselves against this destructive pest at molecular and biochemical levels. Here, we observed the transcriptomic and metabolomic differences in rice leaves after 0, 1, 6, 12, and 24 h of being fed by C. medinalis using RNA sequencing and metabolome profiling. Transcriptional analyses showed that gene expression responds rapidly to leaf folder infestation, with the most significant transcriptional changes occurring within 6 h after the initiation of feeding. Metabolite abundance changed more slowly than gene expression. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that the rice transcriptional response to infestation involved genes encoding protein kinases, transcription factors, biosynthesis of secondary metabolites, photosynthesis, and phytohormone signaling. Moreover, the jasmonic acid-dependent signaling pathway triggered by leaf folder herbivory played a vital role in rice defense against this pest. Taken together, our results provide comprehensive insights into the defense system of rice to this species and may inform the development of insect-resistant rice varieties. MDPI 2020-10-15 /pmc/articles/PMC7602657/ /pubmed/33076419 http://dx.doi.org/10.3390/insects11100705 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yuqi
Liu, Qingsong
Du, Lixiao
Hallerman, Eric M.
Li, Yunhe
Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation
title Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation
title_full Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation
title_fullStr Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation
title_full_unstemmed Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation
title_short Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation
title_sort transcriptomic and metabolomic responses of rice plants to cnaphalocrocis medinalis caterpillar infestation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602657/
https://www.ncbi.nlm.nih.gov/pubmed/33076419
http://dx.doi.org/10.3390/insects11100705
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