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Novel Photosensitizer β-Mannose-Conjugated Chlorin e6 as a Potent Anticancer Agent for Human Glioblastoma U251 Cells

A photosensitizer is a molecular drug for photodynamic diagnosis and photodynamic therapy (PDT) against cancer. Many studies have developed photosensitizers, but improvements in their cost, efficacy, and side effects are needed for better PDT of patients. In the present study, we developed a novel p...

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Autores principales: Shinoda, Yo, Kujirai, Kohei, Aoki, Kohei, Morita, Mai, Masuda, Masato, Zhang, Lihao, Kaixin, Zhou, Nomoto, Akihiro, Takahashi, Tsutomu, Tsuneoka, Yayoi, Akimoto, Jiro, Kataoka, Hiromi, Rachi, Rioko, Narumi, Atsushi, Yoshimura, Tomokazu, Yano, Shigenobu, Fujiwara, Yasuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602738/
https://www.ncbi.nlm.nih.gov/pubmed/33081106
http://dx.doi.org/10.3390/ph13100316
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author Shinoda, Yo
Kujirai, Kohei
Aoki, Kohei
Morita, Mai
Masuda, Masato
Zhang, Lihao
Kaixin, Zhou
Nomoto, Akihiro
Takahashi, Tsutomu
Tsuneoka, Yayoi
Akimoto, Jiro
Kataoka, Hiromi
Rachi, Rioko
Narumi, Atsushi
Yoshimura, Tomokazu
Yano, Shigenobu
Fujiwara, Yasuyuki
author_facet Shinoda, Yo
Kujirai, Kohei
Aoki, Kohei
Morita, Mai
Masuda, Masato
Zhang, Lihao
Kaixin, Zhou
Nomoto, Akihiro
Takahashi, Tsutomu
Tsuneoka, Yayoi
Akimoto, Jiro
Kataoka, Hiromi
Rachi, Rioko
Narumi, Atsushi
Yoshimura, Tomokazu
Yano, Shigenobu
Fujiwara, Yasuyuki
author_sort Shinoda, Yo
collection PubMed
description A photosensitizer is a molecular drug for photodynamic diagnosis and photodynamic therapy (PDT) against cancer. Many studies have developed photosensitizers, but improvements in their cost, efficacy, and side effects are needed for better PDT of patients. In the present study, we developed a novel photosensitizer β-mannose-conjugated chlorin e6 (β-M-Ce6) and investigated its PDT effects in human glioblastoma U251 cells. U251 cells were incubated with β-M-Ce6, followed by laser irradiation. Cell viability was determined using the Cell Counting Kit-8 assay. The PDT effects of β-M-Ce6 were compared with those of talaporfin sodium (TS) and our previously reported photosensitizer β-glucose-conjugated chlorin e6 (β-G-Ce6). Cellular uptake of each photosensitizer and subcellular distribution were analyzed by fluorescence microscopy. β-M-Ce6 showed 1000× more potent PDT effects than those of TS, and these were similar to those of β-G-Ce6. β-M-Ce6 accumulation in U251 cells was much faster than TS accumulation and distributed to several organelles such as the Golgi apparatus, mitochondria, and lysosomes. This rapid cellular uptake was inhibited by low temperature, which suggested that β-M-Ce6 uptake uses biological machinery. β-M-Ce6 showed potent PDT anti-cancer effects compared with clinically approved TS, which is a possible candidate as a next generation photosensitizer in cancer therapy.
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spelling pubmed-76027382020-11-01 Novel Photosensitizer β-Mannose-Conjugated Chlorin e6 as a Potent Anticancer Agent for Human Glioblastoma U251 Cells Shinoda, Yo Kujirai, Kohei Aoki, Kohei Morita, Mai Masuda, Masato Zhang, Lihao Kaixin, Zhou Nomoto, Akihiro Takahashi, Tsutomu Tsuneoka, Yayoi Akimoto, Jiro Kataoka, Hiromi Rachi, Rioko Narumi, Atsushi Yoshimura, Tomokazu Yano, Shigenobu Fujiwara, Yasuyuki Pharmaceuticals (Basel) Article A photosensitizer is a molecular drug for photodynamic diagnosis and photodynamic therapy (PDT) against cancer. Many studies have developed photosensitizers, but improvements in their cost, efficacy, and side effects are needed for better PDT of patients. In the present study, we developed a novel photosensitizer β-mannose-conjugated chlorin e6 (β-M-Ce6) and investigated its PDT effects in human glioblastoma U251 cells. U251 cells were incubated with β-M-Ce6, followed by laser irradiation. Cell viability was determined using the Cell Counting Kit-8 assay. The PDT effects of β-M-Ce6 were compared with those of talaporfin sodium (TS) and our previously reported photosensitizer β-glucose-conjugated chlorin e6 (β-G-Ce6). Cellular uptake of each photosensitizer and subcellular distribution were analyzed by fluorescence microscopy. β-M-Ce6 showed 1000× more potent PDT effects than those of TS, and these were similar to those of β-G-Ce6. β-M-Ce6 accumulation in U251 cells was much faster than TS accumulation and distributed to several organelles such as the Golgi apparatus, mitochondria, and lysosomes. This rapid cellular uptake was inhibited by low temperature, which suggested that β-M-Ce6 uptake uses biological machinery. β-M-Ce6 showed potent PDT anti-cancer effects compared with clinically approved TS, which is a possible candidate as a next generation photosensitizer in cancer therapy. MDPI 2020-10-16 /pmc/articles/PMC7602738/ /pubmed/33081106 http://dx.doi.org/10.3390/ph13100316 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shinoda, Yo
Kujirai, Kohei
Aoki, Kohei
Morita, Mai
Masuda, Masato
Zhang, Lihao
Kaixin, Zhou
Nomoto, Akihiro
Takahashi, Tsutomu
Tsuneoka, Yayoi
Akimoto, Jiro
Kataoka, Hiromi
Rachi, Rioko
Narumi, Atsushi
Yoshimura, Tomokazu
Yano, Shigenobu
Fujiwara, Yasuyuki
Novel Photosensitizer β-Mannose-Conjugated Chlorin e6 as a Potent Anticancer Agent for Human Glioblastoma U251 Cells
title Novel Photosensitizer β-Mannose-Conjugated Chlorin e6 as a Potent Anticancer Agent for Human Glioblastoma U251 Cells
title_full Novel Photosensitizer β-Mannose-Conjugated Chlorin e6 as a Potent Anticancer Agent for Human Glioblastoma U251 Cells
title_fullStr Novel Photosensitizer β-Mannose-Conjugated Chlorin e6 as a Potent Anticancer Agent for Human Glioblastoma U251 Cells
title_full_unstemmed Novel Photosensitizer β-Mannose-Conjugated Chlorin e6 as a Potent Anticancer Agent for Human Glioblastoma U251 Cells
title_short Novel Photosensitizer β-Mannose-Conjugated Chlorin e6 as a Potent Anticancer Agent for Human Glioblastoma U251 Cells
title_sort novel photosensitizer β-mannose-conjugated chlorin e6 as a potent anticancer agent for human glioblastoma u251 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602738/
https://www.ncbi.nlm.nih.gov/pubmed/33081106
http://dx.doi.org/10.3390/ph13100316
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