Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer

BACKGROUND: Increasing evidence shows that circular RNAs (circRNAs) are involved in many biological processes, functioning as microRNA (miRNA) sponges. The aim of this study is to identify differentially expressed circRNAs in bladder cancer (BCa). METHODS: The transcriptome of circRNAs in BCa was as...

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Autores principales: Yang, Jun, Chen, Junwen, Wu, Si, Fei, Xiang, Wang, Xia, Wang, Kefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602908/
https://www.ncbi.nlm.nih.gov/pubmed/33149606
http://dx.doi.org/10.2147/OTT.S270747
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author Yang, Jun
Chen, Junwen
Wu, Si
Fei, Xiang
Wang, Xia
Wang, Kefeng
author_facet Yang, Jun
Chen, Junwen
Wu, Si
Fei, Xiang
Wang, Xia
Wang, Kefeng
author_sort Yang, Jun
collection PubMed
description BACKGROUND: Increasing evidence shows that circular RNAs (circRNAs) are involved in many biological processes, functioning as microRNA (miRNA) sponges. The aim of this study is to identify differentially expressed circRNAs in bladder cancer (BCa). METHODS: The transcriptome of circRNAs in BCa was assayed by microarray. Quantitative real-time PCR was performed to verify the results. Then, potential miRNA response elements (MREs) between circRNAs and miRNAs were predicted. Pathway and ontology enrichment analyses were performed to identify mechanisms related to the gene regulation of differentially expressed circRNAs. RESULTS: Three hundred and eighty-six up-regulated and 394 down-regulated circRNAs were identified, and their potential MREs were predicted in BCa. CONCLUSION: The differentially expressed circRNAs indicate that circRNAs could play important roles in the molecular pathogenesis of BCa.
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spelling pubmed-76029082020-11-03 Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer Yang, Jun Chen, Junwen Wu, Si Fei, Xiang Wang, Xia Wang, Kefeng Onco Targets Ther Original Research BACKGROUND: Increasing evidence shows that circular RNAs (circRNAs) are involved in many biological processes, functioning as microRNA (miRNA) sponges. The aim of this study is to identify differentially expressed circRNAs in bladder cancer (BCa). METHODS: The transcriptome of circRNAs in BCa was assayed by microarray. Quantitative real-time PCR was performed to verify the results. Then, potential miRNA response elements (MREs) between circRNAs and miRNAs were predicted. Pathway and ontology enrichment analyses were performed to identify mechanisms related to the gene regulation of differentially expressed circRNAs. RESULTS: Three hundred and eighty-six up-regulated and 394 down-regulated circRNAs were identified, and their potential MREs were predicted in BCa. CONCLUSION: The differentially expressed circRNAs indicate that circRNAs could play important roles in the molecular pathogenesis of BCa. Dove 2020-10-27 /pmc/articles/PMC7602908/ /pubmed/33149606 http://dx.doi.org/10.2147/OTT.S270747 Text en © 2020 Yang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Yang, Jun
Chen, Junwen
Wu, Si
Fei, Xiang
Wang, Xia
Wang, Kefeng
Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer
title Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer
title_full Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer
title_fullStr Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer
title_full_unstemmed Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer
title_short Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer
title_sort microarray expression profiles and bioinformatics analyses reveal aberrant circular rnas expression in bladder cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602908/
https://www.ncbi.nlm.nih.gov/pubmed/33149606
http://dx.doi.org/10.2147/OTT.S270747
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