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Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years
Changes were evaluated over 10 years in the in vitro resistance of human periodontopathic strains of Parvimonas micra to four antibiotics. Subgingival biofilms culture positive for P. micra from 300 United States adults with severe periodontitis in 2006, and from a similar group of 300 patients in 2...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602954/ https://www.ncbi.nlm.nih.gov/pubmed/33080856 http://dx.doi.org/10.3390/antibiotics9100709 |
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author | Rams, Thomas E. Sautter, Jacqueline D. van Winkelhoff, Arie J. |
author_facet | Rams, Thomas E. Sautter, Jacqueline D. van Winkelhoff, Arie J. |
author_sort | Rams, Thomas E. |
collection | PubMed |
description | Changes were evaluated over 10 years in the in vitro resistance of human periodontopathic strains of Parvimonas micra to four antibiotics. Subgingival biofilms culture positive for P. micra from 300 United States adults with severe periodontitis in 2006, and from a similar group of 300 patients in 2016, were plated onto anaerobically incubated enriched Brucella blood agar alone, or supplemented with either doxycycline (4 mg/L), clindamycin (4 mg/L), amoxicillin (8 mg/L), or metronidazole (16 mg/L). P. micra growth on antibiotic-supplemented media indicated in vitro resistance to the evaluated antibiotic concentration. P. micra resistance was significantly more frequent among patients in 2016, as compared to 2006, for doxycycline (11.3% vs. 0.3% patients; 37.7-fold increase), and clindamycin (47.3% vs. 2.0% patients; 23.7-fold increase) (both p < 0.001), whereas resistance to amoxicillin (2.3% vs. 1.0% patients) and metronidazole (0% vs. 0.3% patients) remained low and statistically unchanged between the two patient groups (p-values > 0.05). No P. micra isolates in 2006 or 2016 were jointly resistant in vitro to both amoxicillin and metronidazole. The alarming increases in subgingival P. micra resistance to doxycycline and clindamycin raise serious questions about the empiric use of these antibiotics, either locally or systemically, in the treatment of United States periodontitis patients harboring subgingival P. micra. |
format | Online Article Text |
id | pubmed-7602954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76029542020-11-01 Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years Rams, Thomas E. Sautter, Jacqueline D. van Winkelhoff, Arie J. Antibiotics (Basel) Article Changes were evaluated over 10 years in the in vitro resistance of human periodontopathic strains of Parvimonas micra to four antibiotics. Subgingival biofilms culture positive for P. micra from 300 United States adults with severe periodontitis in 2006, and from a similar group of 300 patients in 2016, were plated onto anaerobically incubated enriched Brucella blood agar alone, or supplemented with either doxycycline (4 mg/L), clindamycin (4 mg/L), amoxicillin (8 mg/L), or metronidazole (16 mg/L). P. micra growth on antibiotic-supplemented media indicated in vitro resistance to the evaluated antibiotic concentration. P. micra resistance was significantly more frequent among patients in 2016, as compared to 2006, for doxycycline (11.3% vs. 0.3% patients; 37.7-fold increase), and clindamycin (47.3% vs. 2.0% patients; 23.7-fold increase) (both p < 0.001), whereas resistance to amoxicillin (2.3% vs. 1.0% patients) and metronidazole (0% vs. 0.3% patients) remained low and statistically unchanged between the two patient groups (p-values > 0.05). No P. micra isolates in 2006 or 2016 were jointly resistant in vitro to both amoxicillin and metronidazole. The alarming increases in subgingival P. micra resistance to doxycycline and clindamycin raise serious questions about the empiric use of these antibiotics, either locally or systemically, in the treatment of United States periodontitis patients harboring subgingival P. micra. MDPI 2020-10-17 /pmc/articles/PMC7602954/ /pubmed/33080856 http://dx.doi.org/10.3390/antibiotics9100709 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rams, Thomas E. Sautter, Jacqueline D. van Winkelhoff, Arie J. Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years |
title | Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years |
title_full | Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years |
title_fullStr | Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years |
title_full_unstemmed | Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years |
title_short | Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years |
title_sort | antibiotic resistance of human periodontal pathogen parvimonas micra over 10 years |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602954/ https://www.ncbi.nlm.nih.gov/pubmed/33080856 http://dx.doi.org/10.3390/antibiotics9100709 |
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