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Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide
We aim to examine the effects of a newly developed peptide derived from CPNE7 (Cpne7-DP) in tertiary dentin formation and peritubular space occlusion, and comprehensively evaluate its potential as a bioactive therapeutic agent. Human dental pulp cells (HDPCs) and a mouse pre-odontoblast cell line, M...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603008/ https://www.ncbi.nlm.nih.gov/pubmed/33081300 http://dx.doi.org/10.3390/ma13204618 |
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author | Lee, Yoon Seon Park, Yeoung-Hyun Lee, Dong-Seol Seo, You-Mi Lee, Ji-Hyun Park, Joo-Hwang Choung, Han-Wool Park, So-Hyun Shon, Won Jun Park, Joo-Cheol |
author_facet | Lee, Yoon Seon Park, Yeoung-Hyun Lee, Dong-Seol Seo, You-Mi Lee, Ji-Hyun Park, Joo-Hwang Choung, Han-Wool Park, So-Hyun Shon, Won Jun Park, Joo-Cheol |
author_sort | Lee, Yoon Seon |
collection | PubMed |
description | We aim to examine the effects of a newly developed peptide derived from CPNE7 (Cpne7-DP) in tertiary dentin formation and peritubular space occlusion, and comprehensively evaluate its potential as a bioactive therapeutic agent. Human dental pulp cells (HDPCs) and a mouse pre-odontoblast cell line, MDPC-23, were chosen for in vitro studies to characterize lineage-specific cell responses after Cpne7-DP treatment. Whether Cpne7-DP reproduces the dentin regenerative potential of CPNE7 was tested using a beagle dog model by generating dentinal defects of various degrees in vivo. Peritubular space occlusion was further examined by scanning electron microscopy and microleakage test, while overall mineralization capacity of Cpne7-DP was tested ex vivo. CPNE7 promotes tubular dentin formation under both shallow and deep dentinal defects, and the functional peptide Cpne7-DP induces odontoblast-like differentiation in vitro, mineralization ex vivo, and tubular dentin formation in in vivo beagle dog dentin exposure and pulp exposure models. Moreover, Cpne7-DP leads to peritubular space occlusion and maintains stability under different conditions. We show that CPNE7 and its derivative functional peptide Cpne7-DP promotes dentin regeneration in dentinal defects of various degrees and that the regenerated hard tissue demonstrates the characteristics of true dentin. Limitations of the current dental materials including post-operative hypersensitivity make biological repair of dentin a field of growing interest. Here, we suggest that the dual functions of Cpne7-DP in tubular dentin formation and peritubular space occlusion are promising for the treatment of dentinal loss and sensitivity. |
format | Online Article Text |
id | pubmed-7603008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76030082020-11-01 Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide Lee, Yoon Seon Park, Yeoung-Hyun Lee, Dong-Seol Seo, You-Mi Lee, Ji-Hyun Park, Joo-Hwang Choung, Han-Wool Park, So-Hyun Shon, Won Jun Park, Joo-Cheol Materials (Basel) Article We aim to examine the effects of a newly developed peptide derived from CPNE7 (Cpne7-DP) in tertiary dentin formation and peritubular space occlusion, and comprehensively evaluate its potential as a bioactive therapeutic agent. Human dental pulp cells (HDPCs) and a mouse pre-odontoblast cell line, MDPC-23, were chosen for in vitro studies to characterize lineage-specific cell responses after Cpne7-DP treatment. Whether Cpne7-DP reproduces the dentin regenerative potential of CPNE7 was tested using a beagle dog model by generating dentinal defects of various degrees in vivo. Peritubular space occlusion was further examined by scanning electron microscopy and microleakage test, while overall mineralization capacity of Cpne7-DP was tested ex vivo. CPNE7 promotes tubular dentin formation under both shallow and deep dentinal defects, and the functional peptide Cpne7-DP induces odontoblast-like differentiation in vitro, mineralization ex vivo, and tubular dentin formation in in vivo beagle dog dentin exposure and pulp exposure models. Moreover, Cpne7-DP leads to peritubular space occlusion and maintains stability under different conditions. We show that CPNE7 and its derivative functional peptide Cpne7-DP promotes dentin regeneration in dentinal defects of various degrees and that the regenerated hard tissue demonstrates the characteristics of true dentin. Limitations of the current dental materials including post-operative hypersensitivity make biological repair of dentin a field of growing interest. Here, we suggest that the dual functions of Cpne7-DP in tubular dentin formation and peritubular space occlusion are promising for the treatment of dentinal loss and sensitivity. MDPI 2020-10-16 /pmc/articles/PMC7603008/ /pubmed/33081300 http://dx.doi.org/10.3390/ma13204618 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Yoon Seon Park, Yeoung-Hyun Lee, Dong-Seol Seo, You-Mi Lee, Ji-Hyun Park, Joo-Hwang Choung, Han-Wool Park, So-Hyun Shon, Won Jun Park, Joo-Cheol Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide |
title | Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide |
title_full | Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide |
title_fullStr | Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide |
title_full_unstemmed | Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide |
title_short | Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide |
title_sort | tubular dentin regeneration using a cpne7-derived functional peptide |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603008/ https://www.ncbi.nlm.nih.gov/pubmed/33081300 http://dx.doi.org/10.3390/ma13204618 |
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