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Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability

G-rich DNA repeats that can form G-quadruplex structures are prevalent in bacterial genomes and are frequently associated with regulatory regions of genes involved in virulence, antigenic variation, and antibiotic resistance. These sequences are also inherently mutagenic and can lead to changes affe...

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Autores principales: Parekh, Virali J., Wien, Frank, Grange, Wilfried, De Long, Thomas A., Arluison, Véronique, Sinden, Richard R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603069/
https://www.ncbi.nlm.nih.gov/pubmed/33080799
http://dx.doi.org/10.3390/microorganisms8101598
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author Parekh, Virali J.
Wien, Frank
Grange, Wilfried
De Long, Thomas A.
Arluison, Véronique
Sinden, Richard R.
author_facet Parekh, Virali J.
Wien, Frank
Grange, Wilfried
De Long, Thomas A.
Arluison, Véronique
Sinden, Richard R.
author_sort Parekh, Virali J.
collection PubMed
description G-rich DNA repeats that can form G-quadruplex structures are prevalent in bacterial genomes and are frequently associated with regulatory regions of genes involved in virulence, antigenic variation, and antibiotic resistance. These sequences are also inherently mutagenic and can lead to changes affecting cell survival and adaptation. Transcription of the G-quadruplex-forming repeat (G(3)T)(n) in E. coli, when mRNA comprised the G-rich strand, promotes G-quadruplex formation in DNA and increases rates of deletion of G-quadruplex-forming sequences. The genomic instability of G-quadruplex repeats may be a source of genetic variability that can influence alterations and evolution of bacteria. The DNA chaperone Hfq is involved in the genetic instability of these G-quadruplex sequences. Inactivation of the hfq gene decreases the genetic instability of G-quadruplex, demonstrating that the genomic instability of this regulatory element can be influenced by the E. coli highly pleiotropic Hfq protein, which is involved in small noncoding RNA regulation pathways, and DNA organization and packaging. We have shown previously that the protein binds to and stabilizes these sequences, increasing rates of their genomic instability. Here, we extend this analysis to characterize the role of the C-terminal domain of Hfq protein in interaction with G-quadruplex structures. This allows to better understand the function of this specific region of the Hfq protein in genomic instability.
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spelling pubmed-76030692020-11-01 Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability Parekh, Virali J. Wien, Frank Grange, Wilfried De Long, Thomas A. Arluison, Véronique Sinden, Richard R. Microorganisms Article G-rich DNA repeats that can form G-quadruplex structures are prevalent in bacterial genomes and are frequently associated with regulatory regions of genes involved in virulence, antigenic variation, and antibiotic resistance. These sequences are also inherently mutagenic and can lead to changes affecting cell survival and adaptation. Transcription of the G-quadruplex-forming repeat (G(3)T)(n) in E. coli, when mRNA comprised the G-rich strand, promotes G-quadruplex formation in DNA and increases rates of deletion of G-quadruplex-forming sequences. The genomic instability of G-quadruplex repeats may be a source of genetic variability that can influence alterations and evolution of bacteria. The DNA chaperone Hfq is involved in the genetic instability of these G-quadruplex sequences. Inactivation of the hfq gene decreases the genetic instability of G-quadruplex, demonstrating that the genomic instability of this regulatory element can be influenced by the E. coli highly pleiotropic Hfq protein, which is involved in small noncoding RNA regulation pathways, and DNA organization and packaging. We have shown previously that the protein binds to and stabilizes these sequences, increasing rates of their genomic instability. Here, we extend this analysis to characterize the role of the C-terminal domain of Hfq protein in interaction with G-quadruplex structures. This allows to better understand the function of this specific region of the Hfq protein in genomic instability. MDPI 2020-10-17 /pmc/articles/PMC7603069/ /pubmed/33080799 http://dx.doi.org/10.3390/microorganisms8101598 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parekh, Virali J.
Wien, Frank
Grange, Wilfried
De Long, Thomas A.
Arluison, Véronique
Sinden, Richard R.
Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability
title Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability
title_full Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability
title_fullStr Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability
title_full_unstemmed Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability
title_short Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability
title_sort crucial role of the c-terminal domain of hfq protein in genomic instability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603069/
https://www.ncbi.nlm.nih.gov/pubmed/33080799
http://dx.doi.org/10.3390/microorganisms8101598
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