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Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis

Histology, immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) have been used to diagnose feline infectious peritonitis (FIP), but no information regarding the comparison of their diagnostic performances on the same organ is available. The aims of this study were...

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Autores principales: Stranieri, Angelica, Scavone, Donatella, Paltrinieri, Saverio, Giordano, Alessia, Bonsembiante, Federico, Ferro, Silvia, Gelain, Maria Elena, Meazzi, Sara, Lauzi, Stefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603141/
https://www.ncbi.nlm.nih.gov/pubmed/33081040
http://dx.doi.org/10.3390/pathogens9100852
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author Stranieri, Angelica
Scavone, Donatella
Paltrinieri, Saverio
Giordano, Alessia
Bonsembiante, Federico
Ferro, Silvia
Gelain, Maria Elena
Meazzi, Sara
Lauzi, Stefania
author_facet Stranieri, Angelica
Scavone, Donatella
Paltrinieri, Saverio
Giordano, Alessia
Bonsembiante, Federico
Ferro, Silvia
Gelain, Maria Elena
Meazzi, Sara
Lauzi, Stefania
author_sort Stranieri, Angelica
collection PubMed
description Histology, immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) have been used to diagnose feline infectious peritonitis (FIP), but no information regarding the comparison of their diagnostic performances on the same organ is available. The aims of this study were to determine the concordance among these tests and to evaluate which combination of tests and organs can be used in vivo. Histology, IHC, and nested RT-PCR (RT-nPCR) for feline coronavirus (FCoV) were performed on spleen, liver, mesenteric lymph node, kidney, large and small intestine, and lung from 14 FIP and 12 non-FIP cats. Sensitivity, specificity, predictive values, likelihood ratios, and concordance were calculated. IHC and RT-nPCR had the highest concordance in lung and liver, histology and IHC in the other organs. The sensitivity of histology, IHC, and RT-nPCR on the different organs ranged from 41.7 to 76.9%, 46.2 to 76.9%, and 64.3 to 85.7%, respectively, and their specificity ranged from 83.3 to 100.0%, 100% and 83.3 to 100.0%. Therefore, IHC is recommended when histology is consistent with FIP. If RT-nPCR is performed as the first diagnostic approach, results should always be confirmed with IHC. Lung or liver provide accurate information regardless of the method, while IHC is preferred to RT-nPCR to confirm FIP in the kidney or intestine.
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spelling pubmed-76031412020-11-01 Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis Stranieri, Angelica Scavone, Donatella Paltrinieri, Saverio Giordano, Alessia Bonsembiante, Federico Ferro, Silvia Gelain, Maria Elena Meazzi, Sara Lauzi, Stefania Pathogens Article Histology, immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) have been used to diagnose feline infectious peritonitis (FIP), but no information regarding the comparison of their diagnostic performances on the same organ is available. The aims of this study were to determine the concordance among these tests and to evaluate which combination of tests and organs can be used in vivo. Histology, IHC, and nested RT-PCR (RT-nPCR) for feline coronavirus (FCoV) were performed on spleen, liver, mesenteric lymph node, kidney, large and small intestine, and lung from 14 FIP and 12 non-FIP cats. Sensitivity, specificity, predictive values, likelihood ratios, and concordance were calculated. IHC and RT-nPCR had the highest concordance in lung and liver, histology and IHC in the other organs. The sensitivity of histology, IHC, and RT-nPCR on the different organs ranged from 41.7 to 76.9%, 46.2 to 76.9%, and 64.3 to 85.7%, respectively, and their specificity ranged from 83.3 to 100.0%, 100% and 83.3 to 100.0%. Therefore, IHC is recommended when histology is consistent with FIP. If RT-nPCR is performed as the first diagnostic approach, results should always be confirmed with IHC. Lung or liver provide accurate information regardless of the method, while IHC is preferred to RT-nPCR to confirm FIP in the kidney or intestine. MDPI 2020-10-18 /pmc/articles/PMC7603141/ /pubmed/33081040 http://dx.doi.org/10.3390/pathogens9100852 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stranieri, Angelica
Scavone, Donatella
Paltrinieri, Saverio
Giordano, Alessia
Bonsembiante, Federico
Ferro, Silvia
Gelain, Maria Elena
Meazzi, Sara
Lauzi, Stefania
Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
title Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
title_full Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
title_fullStr Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
title_full_unstemmed Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
title_short Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis
title_sort concordance between histology, immunohistochemistry, and rt-pcr in the diagnosis of feline infectious peritonitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603141/
https://www.ncbi.nlm.nih.gov/pubmed/33081040
http://dx.doi.org/10.3390/pathogens9100852
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