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Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes
Most linear peptides directly interact with membranes, but the mechanisms of interaction are far from being completely understood. Here, we present an investigation of the membrane interactions of a designed peptide containing a non-natural, synthetic amino acid. We selected a nonapeptide that is re...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603383/ https://www.ncbi.nlm.nih.gov/pubmed/33086635 http://dx.doi.org/10.3390/membranes10100294 |
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author | Sessa, Lucia Concilio, Simona Walde, Peter Robinson, Tom Dittrich, Petra S. Porta, Amalia Panunzi, Barbara Caruso, Ugo Piotto, Stefano |
author_facet | Sessa, Lucia Concilio, Simona Walde, Peter Robinson, Tom Dittrich, Petra S. Porta, Amalia Panunzi, Barbara Caruso, Ugo Piotto, Stefano |
author_sort | Sessa, Lucia |
collection | PubMed |
description | Most linear peptides directly interact with membranes, but the mechanisms of interaction are far from being completely understood. Here, we present an investigation of the membrane interactions of a designed peptide containing a non-natural, synthetic amino acid. We selected a nonapeptide that is reported to interact with phospholipid membranes, ALYLAIRKR, abbreviated as ALY. We designed a modified peptide (azoALY) by substituting the tyrosine residue of ALY with an antimicrobial azobenzene-bearing amino acid. Both of the peptides were examined for their ability to interact with model membranes, assessing the penetration of phospholipid monolayers, and leakage across the bilayer of large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs). The latter was performed in a microfluidic device in order to study the kinetics of leakage of entrapped calcein from the vesicles at the single vesicle level. Both types of vesicles were prepared from a 9:1 (mol/mol) mixture of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(1′-rac-glycerol). Calcein leakage from the vesicles was more pronounced at a low concentration in the case of azoALY than for ALY. Increased vesicle membrane disturbance in the presence of azoALY was also evident from an enzymatic assay with LUVs and entrapped horseradish peroxidase. Molecular dynamics simulations of ALY and azoALY in an anionic POPC/POPG model bilayer showed that ALY peptide only interacts with the lipid head groups. In contrast, azoALY penetrates the hydrophobic core of the bilayers causing a stronger membrane perturbation as compared to ALY, in qualitative agreement with the experimental results from the leakage assays. |
format | Online Article Text |
id | pubmed-7603383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76033832020-11-01 Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes Sessa, Lucia Concilio, Simona Walde, Peter Robinson, Tom Dittrich, Petra S. Porta, Amalia Panunzi, Barbara Caruso, Ugo Piotto, Stefano Membranes (Basel) Article Most linear peptides directly interact with membranes, but the mechanisms of interaction are far from being completely understood. Here, we present an investigation of the membrane interactions of a designed peptide containing a non-natural, synthetic amino acid. We selected a nonapeptide that is reported to interact with phospholipid membranes, ALYLAIRKR, abbreviated as ALY. We designed a modified peptide (azoALY) by substituting the tyrosine residue of ALY with an antimicrobial azobenzene-bearing amino acid. Both of the peptides were examined for their ability to interact with model membranes, assessing the penetration of phospholipid monolayers, and leakage across the bilayer of large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs). The latter was performed in a microfluidic device in order to study the kinetics of leakage of entrapped calcein from the vesicles at the single vesicle level. Both types of vesicles were prepared from a 9:1 (mol/mol) mixture of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(1′-rac-glycerol). Calcein leakage from the vesicles was more pronounced at a low concentration in the case of azoALY than for ALY. Increased vesicle membrane disturbance in the presence of azoALY was also evident from an enzymatic assay with LUVs and entrapped horseradish peroxidase. Molecular dynamics simulations of ALY and azoALY in an anionic POPC/POPG model bilayer showed that ALY peptide only interacts with the lipid head groups. In contrast, azoALY penetrates the hydrophobic core of the bilayers causing a stronger membrane perturbation as compared to ALY, in qualitative agreement with the experimental results from the leakage assays. MDPI 2020-10-19 /pmc/articles/PMC7603383/ /pubmed/33086635 http://dx.doi.org/10.3390/membranes10100294 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sessa, Lucia Concilio, Simona Walde, Peter Robinson, Tom Dittrich, Petra S. Porta, Amalia Panunzi, Barbara Caruso, Ugo Piotto, Stefano Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes |
title | Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes |
title_full | Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes |
title_fullStr | Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes |
title_full_unstemmed | Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes |
title_short | Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes |
title_sort | study of the interaction of a novel semi-synthetic peptide with model lipid membranes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603383/ https://www.ncbi.nlm.nih.gov/pubmed/33086635 http://dx.doi.org/10.3390/membranes10100294 |
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