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The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
Helical growth is an economical way for plant to obtain resources. The classic microtubule–microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell walls. However, the molecular mechanism underly...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603515/ https://www.ncbi.nlm.nih.gov/pubmed/33328443 http://dx.doi.org/10.1038/s41438-020-00402-0 |
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author | Yang, Qihong Wan, Xiaoshuai Wang, Jiaying Zhang, Yuyang Zhang, Junhong Wang, Taotao Yang, Changxian Ye, Zhibiao |
author_facet | Yang, Qihong Wan, Xiaoshuai Wang, Jiaying Zhang, Yuyang Zhang, Junhong Wang, Taotao Yang, Changxian Ye, Zhibiao |
author_sort | Yang, Qihong |
collection | PubMed |
description | Helical growth is an economical way for plant to obtain resources. The classic microtubule–microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell walls. However, the molecular mechanism underlying tomato helical growth remains unknown. Here, we identified a spontaneous tomato helical (hel) mutant with right-handed helical cotyledons and petals but left-handed helical stems and true leaves. Genetic analysis revealed that the hel phenotype was controlled by a single recessive gene. Using map-based cloning, we cloned the HEL gene, which encodes a cellulose interacting protein homologous to CSI1 of Arabidopsis. We identified a 27 bp fragment replacement that generated a premature stop codon. Transgenic experiments showed that the helical growth phenotype could be restored by the allele of this gene from wild-type Pyriforme. In contrast, the knockout mutation of HEL in Pyriforme via CRISPR/Cas9 resulted in helical growth. These findings shed light on the molecular control of the helical growth of tomato. |
format | Online Article Text |
id | pubmed-7603515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76035152020-11-02 The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato Yang, Qihong Wan, Xiaoshuai Wang, Jiaying Zhang, Yuyang Zhang, Junhong Wang, Taotao Yang, Changxian Ye, Zhibiao Hortic Res Article Helical growth is an economical way for plant to obtain resources. The classic microtubule–microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell walls. However, the molecular mechanism underlying tomato helical growth remains unknown. Here, we identified a spontaneous tomato helical (hel) mutant with right-handed helical cotyledons and petals but left-handed helical stems and true leaves. Genetic analysis revealed that the hel phenotype was controlled by a single recessive gene. Using map-based cloning, we cloned the HEL gene, which encodes a cellulose interacting protein homologous to CSI1 of Arabidopsis. We identified a 27 bp fragment replacement that generated a premature stop codon. Transgenic experiments showed that the helical growth phenotype could be restored by the allele of this gene from wild-type Pyriforme. In contrast, the knockout mutation of HEL in Pyriforme via CRISPR/Cas9 resulted in helical growth. These findings shed light on the molecular control of the helical growth of tomato. Nature Publishing Group UK 2020-11-01 /pmc/articles/PMC7603515/ /pubmed/33328443 http://dx.doi.org/10.1038/s41438-020-00402-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yang, Qihong Wan, Xiaoshuai Wang, Jiaying Zhang, Yuyang Zhang, Junhong Wang, Taotao Yang, Changxian Ye, Zhibiao The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato |
title | The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato |
title_full | The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato |
title_fullStr | The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato |
title_full_unstemmed | The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato |
title_short | The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato |
title_sort | loss of function of hel, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603515/ https://www.ncbi.nlm.nih.gov/pubmed/33328443 http://dx.doi.org/10.1038/s41438-020-00402-0 |
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