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The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato

Helical growth is an economical way for plant to obtain resources. The classic microtubule–microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell walls. However, the molecular mechanism underly...

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Autores principales: Yang, Qihong, Wan, Xiaoshuai, Wang, Jiaying, Zhang, Yuyang, Zhang, Junhong, Wang, Taotao, Yang, Changxian, Ye, Zhibiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603515/
https://www.ncbi.nlm.nih.gov/pubmed/33328443
http://dx.doi.org/10.1038/s41438-020-00402-0
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author Yang, Qihong
Wan, Xiaoshuai
Wang, Jiaying
Zhang, Yuyang
Zhang, Junhong
Wang, Taotao
Yang, Changxian
Ye, Zhibiao
author_facet Yang, Qihong
Wan, Xiaoshuai
Wang, Jiaying
Zhang, Yuyang
Zhang, Junhong
Wang, Taotao
Yang, Changxian
Ye, Zhibiao
author_sort Yang, Qihong
collection PubMed
description Helical growth is an economical way for plant to obtain resources. The classic microtubule–microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell walls. However, the molecular mechanism underlying tomato helical growth remains unknown. Here, we identified a spontaneous tomato helical (hel) mutant with right-handed helical cotyledons and petals but left-handed helical stems and true leaves. Genetic analysis revealed that the hel phenotype was controlled by a single recessive gene. Using map-based cloning, we cloned the HEL gene, which encodes a cellulose interacting protein homologous to CSI1 of Arabidopsis. We identified a 27 bp fragment replacement that generated a premature stop codon. Transgenic experiments showed that the helical growth phenotype could be restored by the allele of this gene from wild-type Pyriforme. In contrast, the knockout mutation of HEL in Pyriforme via CRISPR/Cas9 resulted in helical growth. These findings shed light on the molecular control of the helical growth of tomato.
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spelling pubmed-76035152020-11-02 The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato Yang, Qihong Wan, Xiaoshuai Wang, Jiaying Zhang, Yuyang Zhang, Junhong Wang, Taotao Yang, Changxian Ye, Zhibiao Hortic Res Article Helical growth is an economical way for plant to obtain resources. The classic microtubule–microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell walls. However, the molecular mechanism underlying tomato helical growth remains unknown. Here, we identified a spontaneous tomato helical (hel) mutant with right-handed helical cotyledons and petals but left-handed helical stems and true leaves. Genetic analysis revealed that the hel phenotype was controlled by a single recessive gene. Using map-based cloning, we cloned the HEL gene, which encodes a cellulose interacting protein homologous to CSI1 of Arabidopsis. We identified a 27 bp fragment replacement that generated a premature stop codon. Transgenic experiments showed that the helical growth phenotype could be restored by the allele of this gene from wild-type Pyriforme. In contrast, the knockout mutation of HEL in Pyriforme via CRISPR/Cas9 resulted in helical growth. These findings shed light on the molecular control of the helical growth of tomato. Nature Publishing Group UK 2020-11-01 /pmc/articles/PMC7603515/ /pubmed/33328443 http://dx.doi.org/10.1038/s41438-020-00402-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Qihong
Wan, Xiaoshuai
Wang, Jiaying
Zhang, Yuyang
Zhang, Junhong
Wang, Taotao
Yang, Changxian
Ye, Zhibiao
The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
title The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
title_full The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
title_fullStr The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
title_full_unstemmed The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
title_short The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
title_sort loss of function of hel, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603515/
https://www.ncbi.nlm.nih.gov/pubmed/33328443
http://dx.doi.org/10.1038/s41438-020-00402-0
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