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Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force

PURPOSE: The production of nano-erythrosomes (NEs) by extrusion, which is considered the “gold standard”, has several disadvantages such as difficult equipment assembly, long procedure time, variable pressure, and problems with sterility. An alternative approach, using ultrasound probe, has been sho...

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Detalles Bibliográficos
Autores principales: Capossela, Simona, Mathew, Vikas, Boos, Manuela, Bertolo, Alessandro, Krupkova, Olga, Stoyanov, Jivko V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7604965/
https://www.ncbi.nlm.nih.gov/pubmed/33149552
http://dx.doi.org/10.2147/DDDT.S258368
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author Capossela, Simona
Mathew, Vikas
Boos, Manuela
Bertolo, Alessandro
Krupkova, Olga
Stoyanov, Jivko V
author_facet Capossela, Simona
Mathew, Vikas
Boos, Manuela
Bertolo, Alessandro
Krupkova, Olga
Stoyanov, Jivko V
author_sort Capossela, Simona
collection PubMed
description PURPOSE: The production of nano-erythrosomes (NEs) by extrusion, which is considered the “gold standard”, has several disadvantages such as difficult equipment assembly, long procedure time, variable pressure, and problems with sterility. An alternative approach, using ultrasound probe, has been shown to overheat the sample and have suboptimal results compared to the extrusion method. In our study, we propose, develop, and test a new method for the fabrication of NEs based on shear force and then compare it to the “gold standard” extrusion approach. METHODS: The new method consists of mechanical shear force disruption of the hemoglobin-depleted erythrocyte ghost membranes, with the aid of a rotor stator based tissue homogenizer. Using the same batches of erythrocyte ghost membranes, we compared NEs produced by shear force to NEs produced by the well-established extrusion approach. NEs were characterized for yield, size, encapsulation efficiency, morphology, and stability by flow cytometry (FC), transmission electron microscopy (TEM), and zeta potential analysis. RESULTS: The shear force based process was easier to set up, significantly faster, had better sterility control, and decreased variability between batches. The shear force method generated NEs with the desired size distribution (particles diameter ~125 nm), which were morphologically and functionally equivalent to the NEs produced by extrusion. NEs produced by shear force were stable in terms of counts, size, and fluorescence intensity for 3 weeks at +4°C. Moreover, they showed colloidal stability and minimal influence to centrifugal stress, turbulence shock, and hemolytic potential. CONCLUSION: The newly proposed shear force method allows faster, easier, and highly reproducible NEs production when compared to the conventional extrusion approach. The new setup allows simultaneous production of sterile batches of NEs, which have homogenous size distribution, good stability, and improved shelf life storage. The ability of the shear force method to process also high concentration samples indicates a future potential development of large-scale NEs production and industrial application, which has been a challenge for the extrusion method.
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spelling pubmed-76049652020-11-03 Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force Capossela, Simona Mathew, Vikas Boos, Manuela Bertolo, Alessandro Krupkova, Olga Stoyanov, Jivko V Drug Des Devel Ther Methodology PURPOSE: The production of nano-erythrosomes (NEs) by extrusion, which is considered the “gold standard”, has several disadvantages such as difficult equipment assembly, long procedure time, variable pressure, and problems with sterility. An alternative approach, using ultrasound probe, has been shown to overheat the sample and have suboptimal results compared to the extrusion method. In our study, we propose, develop, and test a new method for the fabrication of NEs based on shear force and then compare it to the “gold standard” extrusion approach. METHODS: The new method consists of mechanical shear force disruption of the hemoglobin-depleted erythrocyte ghost membranes, with the aid of a rotor stator based tissue homogenizer. Using the same batches of erythrocyte ghost membranes, we compared NEs produced by shear force to NEs produced by the well-established extrusion approach. NEs were characterized for yield, size, encapsulation efficiency, morphology, and stability by flow cytometry (FC), transmission electron microscopy (TEM), and zeta potential analysis. RESULTS: The shear force based process was easier to set up, significantly faster, had better sterility control, and decreased variability between batches. The shear force method generated NEs with the desired size distribution (particles diameter ~125 nm), which were morphologically and functionally equivalent to the NEs produced by extrusion. NEs produced by shear force were stable in terms of counts, size, and fluorescence intensity for 3 weeks at +4°C. Moreover, they showed colloidal stability and minimal influence to centrifugal stress, turbulence shock, and hemolytic potential. CONCLUSION: The newly proposed shear force method allows faster, easier, and highly reproducible NEs production when compared to the conventional extrusion approach. The new setup allows simultaneous production of sterile batches of NEs, which have homogenous size distribution, good stability, and improved shelf life storage. The ability of the shear force method to process also high concentration samples indicates a future potential development of large-scale NEs production and industrial application, which has been a challenge for the extrusion method. Dove 2020-10-28 /pmc/articles/PMC7604965/ /pubmed/33149552 http://dx.doi.org/10.2147/DDDT.S258368 Text en © 2020 Capossela et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Methodology
Capossela, Simona
Mathew, Vikas
Boos, Manuela
Bertolo, Alessandro
Krupkova, Olga
Stoyanov, Jivko V
Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force
title Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force
title_full Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force
title_fullStr Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force
title_full_unstemmed Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force
title_short Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force
title_sort novel fast and reliable method for nano-erythrosome production using shear force
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7604965/
https://www.ncbi.nlm.nih.gov/pubmed/33149552
http://dx.doi.org/10.2147/DDDT.S258368
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