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Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis
Sex differences in adipose tissue distribution and function are associated with sex differences in cardiometabolic disease. While many studies have revealed sex differences in adipocyte cell signaling and physiology, there is a relative dearth of information regarding sex differences in transcript a...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605264/ https://www.ncbi.nlm.nih.gov/pubmed/32967914 http://dx.doi.org/10.1101/gr.264614.120 |
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author | Anderson, Warren D. Soh, Joon Yuhl Innis, Sarah E. Dimanche, Alexis Ma, Lijiang Langefeld, Carl D. Comeau, Mary E. Das, Swapan K. Schadt, Eric E. Björkegren, Johan L.M. Civelek, Mete |
author_facet | Anderson, Warren D. Soh, Joon Yuhl Innis, Sarah E. Dimanche, Alexis Ma, Lijiang Langefeld, Carl D. Comeau, Mary E. Das, Swapan K. Schadt, Eric E. Björkegren, Johan L.M. Civelek, Mete |
author_sort | Anderson, Warren D. |
collection | PubMed |
description | Sex differences in adipose tissue distribution and function are associated with sex differences in cardiometabolic disease. While many studies have revealed sex differences in adipocyte cell signaling and physiology, there is a relative dearth of information regarding sex differences in transcript abundance and regulation. We investigated sex differences in subcutaneous adipose tissue transcriptional regulation using omic-scale data from ∼3000 geographically and ethnically diverse human samples. We identified 162 genes with robust sex differences in expression. Differentially expressed genes were implicated in oxidative phosphorylation and adipogenesis. We further determined that sex differences in gene expression levels could be related to sex differences in the genetics of gene expression regulation. Our analyses revealed sex-specific genetic associations, and this finding was replicated in a study of 98 inbred mouse strains. The genes under genetic regulation in human and mouse were enriched for oxidative phosphorylation and adipogenesis. Enrichment analysis showed that the associated genetic loci resided within binding motifs for adipogenic transcription factors (e.g., PPARG and EGR1). We demonstrated that sex differences in gene expression could be influenced by sex differences in genetic regulation for six genes (e.g., FADS1 and MAP1B). These genes exhibited dynamic expression patterns during adipogenesis and robust expression in mature human adipocytes. Our results support a role for adipogenesis-related genes in subcutaneous adipose tissue sex differences in the genetic and environmental regulation of gene expression. |
format | Online Article Text |
id | pubmed-7605264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-76052642021-04-01 Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis Anderson, Warren D. Soh, Joon Yuhl Innis, Sarah E. Dimanche, Alexis Ma, Lijiang Langefeld, Carl D. Comeau, Mary E. Das, Swapan K. Schadt, Eric E. Björkegren, Johan L.M. Civelek, Mete Genome Res Research Sex differences in adipose tissue distribution and function are associated with sex differences in cardiometabolic disease. While many studies have revealed sex differences in adipocyte cell signaling and physiology, there is a relative dearth of information regarding sex differences in transcript abundance and regulation. We investigated sex differences in subcutaneous adipose tissue transcriptional regulation using omic-scale data from ∼3000 geographically and ethnically diverse human samples. We identified 162 genes with robust sex differences in expression. Differentially expressed genes were implicated in oxidative phosphorylation and adipogenesis. We further determined that sex differences in gene expression levels could be related to sex differences in the genetics of gene expression regulation. Our analyses revealed sex-specific genetic associations, and this finding was replicated in a study of 98 inbred mouse strains. The genes under genetic regulation in human and mouse were enriched for oxidative phosphorylation and adipogenesis. Enrichment analysis showed that the associated genetic loci resided within binding motifs for adipogenic transcription factors (e.g., PPARG and EGR1). We demonstrated that sex differences in gene expression could be influenced by sex differences in genetic regulation for six genes (e.g., FADS1 and MAP1B). These genes exhibited dynamic expression patterns during adipogenesis and robust expression in mature human adipocytes. Our results support a role for adipogenesis-related genes in subcutaneous adipose tissue sex differences in the genetic and environmental regulation of gene expression. Cold Spring Harbor Laboratory Press 2020-10 /pmc/articles/PMC7605264/ /pubmed/32967914 http://dx.doi.org/10.1101/gr.264614.120 Text en © 2020 Anderson et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Anderson, Warren D. Soh, Joon Yuhl Innis, Sarah E. Dimanche, Alexis Ma, Lijiang Langefeld, Carl D. Comeau, Mary E. Das, Swapan K. Schadt, Eric E. Björkegren, Johan L.M. Civelek, Mete Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis |
title | Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis |
title_full | Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis |
title_fullStr | Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis |
title_full_unstemmed | Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis |
title_short | Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis |
title_sort | sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605264/ https://www.ncbi.nlm.nih.gov/pubmed/32967914 http://dx.doi.org/10.1101/gr.264614.120 |
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