Metabolic labeling of RNA using multiple ribonucleoside analogs enables the simultaneous evaluation of RNA synthesis and degradation rates

Gene expression is determined by a balance between RNA synthesis and RNA degradation. To elucidate the underlying regulatory mechanisms and principles of this, simultaneous measurements of RNA synthesis and degradation are required. Here, we report the development of “Dyrec-seq,” which uses 4-thiour...

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Detalles Bibliográficos
Autores principales: Kawata, Kentaro, Wakida, Hiroyasu, Yamada, Toshimichi, Taniue, Kenzui, Han, Han, Seki, Masahide, Suzuki, Yutaka, Akimitsu, Nobuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605267/
https://www.ncbi.nlm.nih.gov/pubmed/32843354
http://dx.doi.org/10.1101/gr.264408.120

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