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A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1

Candida glabrata is a prominent pathogenic yeast which exhibits a unique ability to survive the harsh environment of host immune cells. In this study, we describe the role of the transcription factor encoded by the gene CAGL0F09229g, here named CgTog1 after its Saccharomyces cerevisiae ortholog, as...

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Autores principales: Pais, Pedro, Vagueiro, Susana, Mil-Homens, Dalila, Pimenta, Andreia I., Viana, Romeu, Okamoto, Michiyo, Chibana, Hiroji, Fialho, Arsénio M., Teixeira, Miguel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605352/
https://www.ncbi.nlm.nih.gov/pubmed/33135521
http://dx.doi.org/10.1080/21505594.2020.1839231
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author Pais, Pedro
Vagueiro, Susana
Mil-Homens, Dalila
Pimenta, Andreia I.
Viana, Romeu
Okamoto, Michiyo
Chibana, Hiroji
Fialho, Arsénio M.
Teixeira, Miguel C.
author_facet Pais, Pedro
Vagueiro, Susana
Mil-Homens, Dalila
Pimenta, Andreia I.
Viana, Romeu
Okamoto, Michiyo
Chibana, Hiroji
Fialho, Arsénio M.
Teixeira, Miguel C.
author_sort Pais, Pedro
collection PubMed
description Candida glabrata is a prominent pathogenic yeast which exhibits a unique ability to survive the harsh environment of host immune cells. In this study, we describe the role of the transcription factor encoded by the gene CAGL0F09229g, here named CgTog1 after its Saccharomyces cerevisiae ortholog, as a new determinant of C. glabrata virulence. Interestingly, Tog1 is absent in the other clinically relevant Candida species (C. albicans, C. parapsilosis, C. tropicalis, C. auris), being exclusive to C. glabrata. CgTog1 was found to be required for oxidative stress resistance and for the modulation of reactive oxygen species inside C. glabrata cells. Also, CgTog1 was observed to be a nuclear protein, whose activity up-regulates the expression of 147 genes and represses 112 genes in C. glabrata cells exposed to H(2)O(2), as revealed through RNA-seq-based transcriptomics analysis. Given the importance of oxidative stress response in the resistance to host immune cells, the effect of CgTOG1 expression in yeast survival upon phagocytosis by Galleria mellonella hemocytes was evaluated, leading to the identification of CgTog1 as a determinant of yeast survival upon phagocytosis. Interestingly, CgTog1 targets include many whose expression changes in C. glabrata cells after engulfment by macrophages, including those involved in reprogrammed carbon metabolism, glyoxylate cycle and fatty acid degradation. In summary, CgTog1 is a new and specific regulator of virulence in C. glabrata, contributing to oxidative stress resistance and survival upon phagocytosis by host immune cells.
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spelling pubmed-76053522020-11-12 A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1 Pais, Pedro Vagueiro, Susana Mil-Homens, Dalila Pimenta, Andreia I. Viana, Romeu Okamoto, Michiyo Chibana, Hiroji Fialho, Arsénio M. Teixeira, Miguel C. Virulence Research Paper Candida glabrata is a prominent pathogenic yeast which exhibits a unique ability to survive the harsh environment of host immune cells. In this study, we describe the role of the transcription factor encoded by the gene CAGL0F09229g, here named CgTog1 after its Saccharomyces cerevisiae ortholog, as a new determinant of C. glabrata virulence. Interestingly, Tog1 is absent in the other clinically relevant Candida species (C. albicans, C. parapsilosis, C. tropicalis, C. auris), being exclusive to C. glabrata. CgTog1 was found to be required for oxidative stress resistance and for the modulation of reactive oxygen species inside C. glabrata cells. Also, CgTog1 was observed to be a nuclear protein, whose activity up-regulates the expression of 147 genes and represses 112 genes in C. glabrata cells exposed to H(2)O(2), as revealed through RNA-seq-based transcriptomics analysis. Given the importance of oxidative stress response in the resistance to host immune cells, the effect of CgTOG1 expression in yeast survival upon phagocytosis by Galleria mellonella hemocytes was evaluated, leading to the identification of CgTog1 as a determinant of yeast survival upon phagocytosis. Interestingly, CgTog1 targets include many whose expression changes in C. glabrata cells after engulfment by macrophages, including those involved in reprogrammed carbon metabolism, glyoxylate cycle and fatty acid degradation. In summary, CgTog1 is a new and specific regulator of virulence in C. glabrata, contributing to oxidative stress resistance and survival upon phagocytosis by host immune cells. Taylor & Francis 2020-10-31 /pmc/articles/PMC7605352/ /pubmed/33135521 http://dx.doi.org/10.1080/21505594.2020.1839231 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Pais, Pedro
Vagueiro, Susana
Mil-Homens, Dalila
Pimenta, Andreia I.
Viana, Romeu
Okamoto, Michiyo
Chibana, Hiroji
Fialho, Arsénio M.
Teixeira, Miguel C.
A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_full A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_fullStr A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_full_unstemmed A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_short A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_sort new regulator in the crossroads of oxidative stress resistance and virulence in candida glabrata: the transcription factor cgtog1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605352/
https://www.ncbi.nlm.nih.gov/pubmed/33135521
http://dx.doi.org/10.1080/21505594.2020.1839231
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