Cargando…

Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering

Porous scaffolds of poly(lactide-co-glycolide) (PLGA; 85:15) and nano-hydroxyapatite (nHAP) were prepared by an emulsion-precipitation procedure from uniform PLGA–nHAP spheres (150–250 µm diameter). These spheres were then thermally sintered at 83 °C to porous scaffolds that can serve for bone tissu...

Descripción completa

Detalles Bibliográficos
Autores principales: Sokolova, Viktoriya, Kostka, Kathrin, Shalumon, K. T., Prymak, Oleg, Chen, Jyh-Ping, Epple, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606283/
https://www.ncbi.nlm.nih.gov/pubmed/33140175
http://dx.doi.org/10.1007/s10856-020-06442-1
_version_ 1783604462364917760
author Sokolova, Viktoriya
Kostka, Kathrin
Shalumon, K. T.
Prymak, Oleg
Chen, Jyh-Ping
Epple, Matthias
author_facet Sokolova, Viktoriya
Kostka, Kathrin
Shalumon, K. T.
Prymak, Oleg
Chen, Jyh-Ping
Epple, Matthias
author_sort Sokolova, Viktoriya
collection PubMed
description Porous scaffolds of poly(lactide-co-glycolide) (PLGA; 85:15) and nano-hydroxyapatite (nHAP) were prepared by an emulsion-precipitation procedure from uniform PLGA–nHAP spheres (150–250 µm diameter). These spheres were then thermally sintered at 83 °C to porous scaffolds that can serve for bone tissue engineering or for bone substitution. The base materials PLGA and nHAP and the PLGA–nHAP scaffolds were extensively characterized by X-ray powder diffraction, infrared spectroscopy, thermogravimetry, differential scanning calorimetry, and scanning electron microscopy. The scaffold porosity was about 50 vol% as determined by relating mass and volume of the scaffolds, together with the computed density of the solid phase (PLGA–nHAP). The cultivation of HeLa cells demonstrated their high cytocompatibility. In combination with DNA-loaded calcium phosphate nanoparticles, they showed a good activity of gene transfection with enhanced green fluorescent protein (EGFP) as model protein. This is expected enhance bone growth around an implanted scaffold or inside a scaffold for tissue engineering. [Image: see text]
format Online
Article
Text
id pubmed-7606283
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-76062832020-11-10 Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering Sokolova, Viktoriya Kostka, Kathrin Shalumon, K. T. Prymak, Oleg Chen, Jyh-Ping Epple, Matthias J Mater Sci Mater Med Tissue Engineering Constructs and Cell Substrates Porous scaffolds of poly(lactide-co-glycolide) (PLGA; 85:15) and nano-hydroxyapatite (nHAP) were prepared by an emulsion-precipitation procedure from uniform PLGA–nHAP spheres (150–250 µm diameter). These spheres were then thermally sintered at 83 °C to porous scaffolds that can serve for bone tissue engineering or for bone substitution. The base materials PLGA and nHAP and the PLGA–nHAP scaffolds were extensively characterized by X-ray powder diffraction, infrared spectroscopy, thermogravimetry, differential scanning calorimetry, and scanning electron microscopy. The scaffold porosity was about 50 vol% as determined by relating mass and volume of the scaffolds, together with the computed density of the solid phase (PLGA–nHAP). The cultivation of HeLa cells demonstrated their high cytocompatibility. In combination with DNA-loaded calcium phosphate nanoparticles, they showed a good activity of gene transfection with enhanced green fluorescent protein (EGFP) as model protein. This is expected enhance bone growth around an implanted scaffold or inside a scaffold for tissue engineering. [Image: see text] Springer US 2020-11-02 2020 /pmc/articles/PMC7606283/ /pubmed/33140175 http://dx.doi.org/10.1007/s10856-020-06442-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Tissue Engineering Constructs and Cell Substrates
Sokolova, Viktoriya
Kostka, Kathrin
Shalumon, K. T.
Prymak, Oleg
Chen, Jyh-Ping
Epple, Matthias
Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering
title Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering
title_full Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering
title_fullStr Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering
title_full_unstemmed Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering
title_short Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering
title_sort synthesis and characterization of plga/hap scaffolds with dna-functionalised calcium phosphate nanoparticles for bone tissue engineering
topic Tissue Engineering Constructs and Cell Substrates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606283/
https://www.ncbi.nlm.nih.gov/pubmed/33140175
http://dx.doi.org/10.1007/s10856-020-06442-1
work_keys_str_mv AT sokolovaviktoriya synthesisandcharacterizationofplgahapscaffoldswithdnafunctionalisedcalciumphosphatenanoparticlesforbonetissueengineering
AT kostkakathrin synthesisandcharacterizationofplgahapscaffoldswithdnafunctionalisedcalciumphosphatenanoparticlesforbonetissueengineering
AT shalumonkt synthesisandcharacterizationofplgahapscaffoldswithdnafunctionalisedcalciumphosphatenanoparticlesforbonetissueengineering
AT prymakoleg synthesisandcharacterizationofplgahapscaffoldswithdnafunctionalisedcalciumphosphatenanoparticlesforbonetissueengineering
AT chenjyhping synthesisandcharacterizationofplgahapscaffoldswithdnafunctionalisedcalciumphosphatenanoparticlesforbonetissueengineering
AT epplematthias synthesisandcharacterizationofplgahapscaffoldswithdnafunctionalisedcalciumphosphatenanoparticlesforbonetissueengineering