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Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons

A battery of genetically encoded calcium indicators (GECIs) with different binding kinetics and calcium affinities was developed over the recent years to permit long-term calcium imaging. GECIs are calcium buffers and therefore, expression of GECIs may interfere with calcium homeostasis and signalin...

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Autores principales: Gasterstädt, Ina, Jack, Alexander, Stahlhut, Tobias, Rennau, Lisa-Marie, Gonda, Steffen, Wahle, Petra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606991/
https://www.ncbi.nlm.nih.gov/pubmed/33192315
http://dx.doi.org/10.3389/fncel.2020.570596
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author Gasterstädt, Ina
Jack, Alexander
Stahlhut, Tobias
Rennau, Lisa-Marie
Gonda, Steffen
Wahle, Petra
author_facet Gasterstädt, Ina
Jack, Alexander
Stahlhut, Tobias
Rennau, Lisa-Marie
Gonda, Steffen
Wahle, Petra
author_sort Gasterstädt, Ina
collection PubMed
description A battery of genetically encoded calcium indicators (GECIs) with different binding kinetics and calcium affinities was developed over the recent years to permit long-term calcium imaging. GECIs are calcium buffers and therefore, expression of GECIs may interfere with calcium homeostasis and signaling pathways important for neuronal differentiation and survival. Our objective was to investigate if the biolistically induced expression of five commonly used GECIs at two postnatal time points (days 14 and 22–25) could affect the morphological maturation of cortical neurons in organotypic slice cultures of rat visual cortex. Expression of GCaMP3 in both time windows, and of GCaMP5G and TN-XXL in the later time window impaired apical and /or basal dendrite growth of pyramidal neurons. With time, the proportion of GECI transfectants with nuclear filling increased, but an only prolonged expression of TN-XXL caused higher levels of neurodegeneration. In multipolar interneurons, only GCaMP3 evoked a transient growth delay during the early time window. GCaMP6m and GCaMP6m-X(C) were quite “neuron-friendly.” Since growth-impaired neurons might not have the physiological responses typical of age-matched wildtype neurons the results obtained after prolonged developmental expression of certain GECIs might need to be interpreted with caution.
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spelling pubmed-76069912020-11-13 Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons Gasterstädt, Ina Jack, Alexander Stahlhut, Tobias Rennau, Lisa-Marie Gonda, Steffen Wahle, Petra Front Cell Neurosci Cellular Neuroscience A battery of genetically encoded calcium indicators (GECIs) with different binding kinetics and calcium affinities was developed over the recent years to permit long-term calcium imaging. GECIs are calcium buffers and therefore, expression of GECIs may interfere with calcium homeostasis and signaling pathways important for neuronal differentiation and survival. Our objective was to investigate if the biolistically induced expression of five commonly used GECIs at two postnatal time points (days 14 and 22–25) could affect the morphological maturation of cortical neurons in organotypic slice cultures of rat visual cortex. Expression of GCaMP3 in both time windows, and of GCaMP5G and TN-XXL in the later time window impaired apical and /or basal dendrite growth of pyramidal neurons. With time, the proportion of GECI transfectants with nuclear filling increased, but an only prolonged expression of TN-XXL caused higher levels of neurodegeneration. In multipolar interneurons, only GCaMP3 evoked a transient growth delay during the early time window. GCaMP6m and GCaMP6m-X(C) were quite “neuron-friendly.” Since growth-impaired neurons might not have the physiological responses typical of age-matched wildtype neurons the results obtained after prolonged developmental expression of certain GECIs might need to be interpreted with caution. Frontiers Media S.A. 2020-10-20 /pmc/articles/PMC7606991/ /pubmed/33192315 http://dx.doi.org/10.3389/fncel.2020.570596 Text en Copyright © 2020 Gasterstädt, Jack, Stahlhut, Rennau, Gonda and Wahle. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Gasterstädt, Ina
Jack, Alexander
Stahlhut, Tobias
Rennau, Lisa-Marie
Gonda, Steffen
Wahle, Petra
Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons
title Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons
title_full Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons
title_fullStr Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons
title_full_unstemmed Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons
title_short Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons
title_sort genetically encoded calcium indicators can impair dendrite growth of cortical neurons
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606991/
https://www.ncbi.nlm.nih.gov/pubmed/33192315
http://dx.doi.org/10.3389/fncel.2020.570596
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