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MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway
OBJECTIVES: The objective was to investigate the effects of microRNA-421 against myocardial ischemia/reperfusion injury in C57BL/6 mice. METHODS: Male C57BL/6 mice (n = 27) were randomly divided into three groups: normal control (NC) group (sham-treated); I/R model group, which underwent the I(30min...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607211/ https://www.ncbi.nlm.nih.gov/pubmed/31847632 http://dx.doi.org/10.1177/0300060519871863 |
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author | Guo, Lin-lin Guo, Ming-lei Yao, Jian Weng, Yun-qi Zhang, Xue-zhi |
author_facet | Guo, Lin-lin Guo, Ming-lei Yao, Jian Weng, Yun-qi Zhang, Xue-zhi |
author_sort | Guo, Lin-lin |
collection | PubMed |
description | OBJECTIVES: The objective was to investigate the effects of microRNA-421 against myocardial ischemia/reperfusion injury in C57BL/6 mice. METHODS: Male C57BL/6 mice (n = 27) were randomly divided into three groups: normal control (NC) group (sham-treated); I/R model group, which underwent the I(30min)/R(24h) model (ischemia for 30 minutes followed by reperfusion for 24 hours); and the miRNA group, which were injected with miR-421. Pathology was assessed by hematoxylin and eosin staining and myocardial infarct size was measured by triphenyltetrazolium chloride staining. The apoptosis rate was measured by TUNEL assay, and relative expression of toll-like receptor-4 (TLR4), Janus kinase 2 (JAK2), and signal transducer and activator of translation 3 (STAT3) was evaluated by immunohistochemistry. Interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-10, and high mobility group protein B1 (HMGB1) serum concentrations were measured by ELISA. RESULTS: Compared with the NC group, in the model group, the myocardial infarction was large; inflammatory cell infiltration was severe; apoptosis was enhanced; expression of TLR4, JAK2, and STAT3 was increased; and serum concentrations of IL-6, TNF-α, IL-10, and HMGB1 were significantly increased. In the miRNA group, the ischemia/reperfusion injury was significantly improved. CONCLUSIONS: Overexpression of miRNA-421 could reduce ischemia/reperfusion inflammatory response, perhaps via inactivation of TLR4, JAK2, and STAT3. |
format | Online Article Text |
id | pubmed-7607211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-76072112020-11-12 MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway Guo, Lin-lin Guo, Ming-lei Yao, Jian Weng, Yun-qi Zhang, Xue-zhi J Int Med Res Prospective Clinical Research Report OBJECTIVES: The objective was to investigate the effects of microRNA-421 against myocardial ischemia/reperfusion injury in C57BL/6 mice. METHODS: Male C57BL/6 mice (n = 27) were randomly divided into three groups: normal control (NC) group (sham-treated); I/R model group, which underwent the I(30min)/R(24h) model (ischemia for 30 minutes followed by reperfusion for 24 hours); and the miRNA group, which were injected with miR-421. Pathology was assessed by hematoxylin and eosin staining and myocardial infarct size was measured by triphenyltetrazolium chloride staining. The apoptosis rate was measured by TUNEL assay, and relative expression of toll-like receptor-4 (TLR4), Janus kinase 2 (JAK2), and signal transducer and activator of translation 3 (STAT3) was evaluated by immunohistochemistry. Interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-10, and high mobility group protein B1 (HMGB1) serum concentrations were measured by ELISA. RESULTS: Compared with the NC group, in the model group, the myocardial infarction was large; inflammatory cell infiltration was severe; apoptosis was enhanced; expression of TLR4, JAK2, and STAT3 was increased; and serum concentrations of IL-6, TNF-α, IL-10, and HMGB1 were significantly increased. In the miRNA group, the ischemia/reperfusion injury was significantly improved. CONCLUSIONS: Overexpression of miRNA-421 could reduce ischemia/reperfusion inflammatory response, perhaps via inactivation of TLR4, JAK2, and STAT3. SAGE Publications 2019-12-18 /pmc/articles/PMC7607211/ /pubmed/31847632 http://dx.doi.org/10.1177/0300060519871863 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Prospective Clinical Research Report Guo, Lin-lin Guo, Ming-lei Yao, Jian Weng, Yun-qi Zhang, Xue-zhi MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway |
title | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like
receptor 4 pathway |
title_full | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like
receptor 4 pathway |
title_fullStr | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like
receptor 4 pathway |
title_full_unstemmed | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like
receptor 4 pathway |
title_short | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like
receptor 4 pathway |
title_sort | microrna-421 improves ischemia/reperfusion injury via regulation toll-like
receptor 4 pathway |
topic | Prospective Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607211/ https://www.ncbi.nlm.nih.gov/pubmed/31847632 http://dx.doi.org/10.1177/0300060519871863 |
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