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miR-4324 functions as a tumor suppressor in colorectal cancer by targeting HOXB2
OBJECTIVE: MicroRNAs (miRNAs) are reported to have crucial roles in human cancers; however, their role in colorectal cancer (CRC) remains largely unknown. METHODS: In this study, we analyzed the expression of miR-4324 in CRC cell lines using reverse transcription-quantitative polymerase chain reacti...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607221/ https://www.ncbi.nlm.nih.gov/pubmed/31852342 http://dx.doi.org/10.1177/0300060519883731 |
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author | Li, Hailin Zhu, Guiling Xing, Yanwei Zhu, Yuekun Piao, Daxun |
author_facet | Li, Hailin Zhu, Guiling Xing, Yanwei Zhu, Yuekun Piao, Daxun |
author_sort | Li, Hailin |
collection | PubMed |
description | OBJECTIVE: MicroRNAs (miRNAs) are reported to have crucial roles in human cancers; however, their role in colorectal cancer (CRC) remains largely unknown. METHODS: In this study, we analyzed the expression of miR-4324 in CRC cell lines using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We also examined miR-4324 expression in CRC tumor tissues using a miRNA expression dataset obtained from the Gene Expression Omnibus. We validated the connection between miR-4324 and homeobox B2 (HOXB2) using a luciferase activity reporter assay and western blotting. The effects of miR-4324 and HOXB2 on CRC cell malignant behaviors in vitro were further investigated. RESULTS: miR-4324 expression was significantly decreased in both CRC tumor tissues and cell lines. Overexpression of miR-4324 suppressed CRC cell proliferation, migration, and invasion. In contrast, overexpression of HOXB2 promoted CRC malignant cell behaviors. Furthermore, we validated HOXB2 as a direct target of miR-4324. CONCLUSIONS: miR-4324 expression was decreased in CRC. miR-4324 regulates CRC cell proliferation, migration, and invasion by targeting HOXB2. |
format | Online Article Text |
id | pubmed-7607221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-76072212020-11-12 miR-4324 functions as a tumor suppressor in colorectal cancer by targeting HOXB2 Li, Hailin Zhu, Guiling Xing, Yanwei Zhu, Yuekun Piao, Daxun J Int Med Res Pre-Clinical Research Report OBJECTIVE: MicroRNAs (miRNAs) are reported to have crucial roles in human cancers; however, their role in colorectal cancer (CRC) remains largely unknown. METHODS: In this study, we analyzed the expression of miR-4324 in CRC cell lines using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We also examined miR-4324 expression in CRC tumor tissues using a miRNA expression dataset obtained from the Gene Expression Omnibus. We validated the connection between miR-4324 and homeobox B2 (HOXB2) using a luciferase activity reporter assay and western blotting. The effects of miR-4324 and HOXB2 on CRC cell malignant behaviors in vitro were further investigated. RESULTS: miR-4324 expression was significantly decreased in both CRC tumor tissues and cell lines. Overexpression of miR-4324 suppressed CRC cell proliferation, migration, and invasion. In contrast, overexpression of HOXB2 promoted CRC malignant cell behaviors. Furthermore, we validated HOXB2 as a direct target of miR-4324. CONCLUSIONS: miR-4324 expression was decreased in CRC. miR-4324 regulates CRC cell proliferation, migration, and invasion by targeting HOXB2. SAGE Publications 2019-12-18 /pmc/articles/PMC7607221/ /pubmed/31852342 http://dx.doi.org/10.1177/0300060519883731 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Pre-Clinical Research Report Li, Hailin Zhu, Guiling Xing, Yanwei Zhu, Yuekun Piao, Daxun miR-4324 functions as a tumor suppressor in colorectal cancer by targeting HOXB2 |
title | miR-4324 functions as a tumor suppressor in colorectal cancer by targeting
HOXB2 |
title_full | miR-4324 functions as a tumor suppressor in colorectal cancer by targeting
HOXB2 |
title_fullStr | miR-4324 functions as a tumor suppressor in colorectal cancer by targeting
HOXB2 |
title_full_unstemmed | miR-4324 functions as a tumor suppressor in colorectal cancer by targeting
HOXB2 |
title_short | miR-4324 functions as a tumor suppressor in colorectal cancer by targeting
HOXB2 |
title_sort | mir-4324 functions as a tumor suppressor in colorectal cancer by targeting
hoxb2 |
topic | Pre-Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607221/ https://www.ncbi.nlm.nih.gov/pubmed/31852342 http://dx.doi.org/10.1177/0300060519883731 |
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