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Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality

Reproductive tract inflammation is considered an important cause of male infertility. Increased leukocytes in semen can produce many reactive oxygen species (ROS), which affect sperm function. The aim of this study is to identify the main source of ROS in seminal plasma and to assess the effect of R...

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Autores principales: Li, Xiaoping, Ni, Mengxia, Xing, Shiyu, Yu, Yi, Zhou, Yan, Yang, Shenmin, Li, Hong, Zhu, Rui, Han, Mutian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607761/
https://www.ncbi.nlm.nih.gov/pubmed/33131385
http://dx.doi.org/10.1177/1557988320970053
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author Li, Xiaoping
Ni, Mengxia
Xing, Shiyu
Yu, Yi
Zhou, Yan
Yang, Shenmin
Li, Hong
Zhu, Rui
Han, Mutian
author_facet Li, Xiaoping
Ni, Mengxia
Xing, Shiyu
Yu, Yi
Zhou, Yan
Yang, Shenmin
Li, Hong
Zhu, Rui
Han, Mutian
author_sort Li, Xiaoping
collection PubMed
description Reproductive tract inflammation is considered an important cause of male infertility. Increased leukocytes in semen can produce many reactive oxygen species (ROS), which affect sperm function. The aim of this study is to identify the main source of ROS in seminal plasma and to assess the effect of ROS on leukocytes. Semen samples (n = 20) with leukocyte concentration >1 × 10(6) were collected from a male infertility clinic. This study mainly compares the sperm function parameters of the normal group and the semen white blood cell group >1 × 10(6). The results identified that ROS in semen was closely related to sperm function parameters, and CD45(+) leucocytes were the main source of ROS. Compared with the control group, the concentration of IL-2, IL-4, IL-6, IFN-γ, and TNF-α was higher in the experimental group. Leukocytes in semen may regulate the secretion of ROS through the mammalian target of rapamycin (mTOR) pathway. A considerable amount of ROS can upregulate the expression of IL-6 in leukocytes via the nuclear factor kappa-B (NF-kB) pathway.
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spelling pubmed-76077612020-11-13 Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality Li, Xiaoping Ni, Mengxia Xing, Shiyu Yu, Yi Zhou, Yan Yang, Shenmin Li, Hong Zhu, Rui Han, Mutian Am J Mens Health Original Article Reproductive tract inflammation is considered an important cause of male infertility. Increased leukocytes in semen can produce many reactive oxygen species (ROS), which affect sperm function. The aim of this study is to identify the main source of ROS in seminal plasma and to assess the effect of ROS on leukocytes. Semen samples (n = 20) with leukocyte concentration >1 × 10(6) were collected from a male infertility clinic. This study mainly compares the sperm function parameters of the normal group and the semen white blood cell group >1 × 10(6). The results identified that ROS in semen was closely related to sperm function parameters, and CD45(+) leucocytes were the main source of ROS. Compared with the control group, the concentration of IL-2, IL-4, IL-6, IFN-γ, and TNF-α was higher in the experimental group. Leukocytes in semen may regulate the secretion of ROS through the mammalian target of rapamycin (mTOR) pathway. A considerable amount of ROS can upregulate the expression of IL-6 in leukocytes via the nuclear factor kappa-B (NF-kB) pathway. SAGE Publications 2020-10-31 /pmc/articles/PMC7607761/ /pubmed/33131385 http://dx.doi.org/10.1177/1557988320970053 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Li, Xiaoping
Ni, Mengxia
Xing, Shiyu
Yu, Yi
Zhou, Yan
Yang, Shenmin
Li, Hong
Zhu, Rui
Han, Mutian
Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality
title Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality
title_full Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality
title_fullStr Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality
title_full_unstemmed Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality
title_short Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality
title_sort reactive oxygen species secreted by leukocytes in semen induce self-expression of interleukin-6 and affect sperm quality
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607761/
https://www.ncbi.nlm.nih.gov/pubmed/33131385
http://dx.doi.org/10.1177/1557988320970053
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