Cargando…

Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway

Gastric cancer (GC) remains a threat to the health of the global population. The present study investigated the effects and mechanisms of the long non-coding RNA myocardial infarction associated transcript (MIAT) on the proliferation, apoptosis and metastasis of GC (HGC-27 and AGS) cells. The expres...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xiao-Mei, Jiao, Yan-Yan, Luan, Bao-Hong, Wu, Hong-Xia, Wang, Rong-Rong, Zhong, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608069/
https://www.ncbi.nlm.nih.gov/pubmed/33154765
http://dx.doi.org/10.3892/ol.2020.12219
_version_ 1783604765063643136
author Li, Xiao-Mei
Jiao, Yan-Yan
Luan, Bao-Hong
Wu, Hong-Xia
Wang, Rong-Rong
Zhong, Jie
author_facet Li, Xiao-Mei
Jiao, Yan-Yan
Luan, Bao-Hong
Wu, Hong-Xia
Wang, Rong-Rong
Zhong, Jie
author_sort Li, Xiao-Mei
collection PubMed
description Gastric cancer (GC) remains a threat to the health of the global population. The present study investigated the effects and mechanisms of the long non-coding RNA myocardial infarction associated transcript (MIAT) on the proliferation, apoptosis and metastasis of GC (HGC-27 and AGS) cells. The expression levels of MIAT, micoRNA (miR)-331-3p and RAB5B mRNA were analyzed using reverse transcription-quantitative PCR analysis. Cell growth, apoptosis, migration and invasion were measured using 5-ethynyl-2′-deoxyuridine, flow cytometry, wound healing and Transwell assays, respectively. A luciferase assay was used to determine whether miR-331-3p targeted MIAT and RAB5B. The results indicated that MIAT levels were significantly upregulated in GC tissues and cells, correlated with RAB5B levels and inversely associated with miR-331-3p levels. MIAT overexpression promoted proliferation and metastasis, and inhibited the apoptosis of GC cells. MIAT knockdown had the opposite effect on GC cells. The rescue experiments revealed that the effects of MIAT knockdown on the biological behaviour of GC cells were attenuated by RAB5B overexpression. These data suggest that MIAT promotes GC progression via modulating miR-331-3p/RAB5B pathway.
format Online
Article
Text
id pubmed-7608069
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-76080692020-11-04 Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway Li, Xiao-Mei Jiao, Yan-Yan Luan, Bao-Hong Wu, Hong-Xia Wang, Rong-Rong Zhong, Jie Oncol Lett Articles Gastric cancer (GC) remains a threat to the health of the global population. The present study investigated the effects and mechanisms of the long non-coding RNA myocardial infarction associated transcript (MIAT) on the proliferation, apoptosis and metastasis of GC (HGC-27 and AGS) cells. The expression levels of MIAT, micoRNA (miR)-331-3p and RAB5B mRNA were analyzed using reverse transcription-quantitative PCR analysis. Cell growth, apoptosis, migration and invasion were measured using 5-ethynyl-2′-deoxyuridine, flow cytometry, wound healing and Transwell assays, respectively. A luciferase assay was used to determine whether miR-331-3p targeted MIAT and RAB5B. The results indicated that MIAT levels were significantly upregulated in GC tissues and cells, correlated with RAB5B levels and inversely associated with miR-331-3p levels. MIAT overexpression promoted proliferation and metastasis, and inhibited the apoptosis of GC cells. MIAT knockdown had the opposite effect on GC cells. The rescue experiments revealed that the effects of MIAT knockdown on the biological behaviour of GC cells were attenuated by RAB5B overexpression. These data suggest that MIAT promotes GC progression via modulating miR-331-3p/RAB5B pathway. D.A. Spandidos 2020-12 2020-10-13 /pmc/articles/PMC7608069/ /pubmed/33154765 http://dx.doi.org/10.3892/ol.2020.12219 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Articles
Li, Xiao-Mei
Jiao, Yan-Yan
Luan, Bao-Hong
Wu, Hong-Xia
Wang, Rong-Rong
Zhong, Jie
Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway
title Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway
title_full Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway
title_fullStr Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway
title_full_unstemmed Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway
title_short Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway
title_sort long non-coding rna miat promotes gastric cancer proliferation and metastasis via modulating the mir-331-3p/rab5b pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608069/
https://www.ncbi.nlm.nih.gov/pubmed/33154765
http://dx.doi.org/10.3892/ol.2020.12219
work_keys_str_mv AT lixiaomei longnoncodingrnamiatpromotesgastriccancerproliferationandmetastasisviamodulatingthemir3313prab5bpathway
AT jiaoyanyan longnoncodingrnamiatpromotesgastriccancerproliferationandmetastasisviamodulatingthemir3313prab5bpathway
AT luanbaohong longnoncodingrnamiatpromotesgastriccancerproliferationandmetastasisviamodulatingthemir3313prab5bpathway
AT wuhongxia longnoncodingrnamiatpromotesgastriccancerproliferationandmetastasisviamodulatingthemir3313prab5bpathway
AT wangrongrong longnoncodingrnamiatpromotesgastriccancerproliferationandmetastasisviamodulatingthemir3313prab5bpathway
AT zhongjie longnoncodingrnamiatpromotesgastriccancerproliferationandmetastasisviamodulatingthemir3313prab5bpathway