Cargando…

Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide

This study aimed to investigate a methodology for discriminating viable and non-viable T. gondii oocysts in water. Analyses included two steps: (i) microscopic investigation with vital dyes; (ii) molecular investigation, using a real time PCR (qPCR), after parasite treatment (or not) with propidium...

Descripción completa

Detalles Bibliográficos
Autores principales:	Marciano, Maria Aparecida Moraes, Silva, Rafaela Aparecida, Barbosa, Maria Luisa, Ferreira, Antônio Roberto Souza, Pereira-Chioccola, Vera Lucia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Instituto de Medicina Tropical 2020
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608075/ https://www.ncbi.nlm.nih.gov/pubmed/33146312 http://dx.doi.org/10.1590/S1678-9946202062084

Ejemplares similares

Assessment of Probiotic Viability during Cheddar Cheese Manufacture and Ripening Using Propidium Monoazide-PCR Quantification
por: Desfossés-Foucault, Émilie, et al.
Publicado: (2012)

Rapid molecular phenotypic antimicrobial susceptibility test for Neisseria gonorrhoeae based on propidium monoazide viability PCR
por: Tjandra, Kristel C., et al.
Publicado: (2023)

Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approach
por: Wang, Ya, et al.
Publicado: (2021)

Development of a Propidium Monoazide-Based Viability Quantitative PCR Assay for Red Sea Bream Iridovirus Detection
por: Kim, Kyung-Ho, et al.
Publicado: (2023)

Detection of suid herpesvirus 1 infectivity in pigs by propidium monoazide-qPCR
por: Yang, Liu, et al.
Publicado: (2022)

Selective detection of viable seed-borne Acidovorax citrulli by real-time PCR with propidium monoazide
por: Tian, Qian, et al.
Publicado: (2016)

Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide‐Modified Quantitative PCR
por: Luo, Yu, et al.
Publicado: (2016)

Propidium Monoazide Combined With RT-qPCR Detects Infectivity of Porcine Epidemic Diarrhea Virus
por: Liang, Gong, et al.
Publicado: (2022)

Viability of Legionella pneumophila in Water Samples: A Comparison of Propidium Monoazide (PMA) Treatment on Membrane Filters and in Liquid
por: Bonetta, Sara, et al.
Publicado: (2017)

Limitations of Using Propidium Monoazide with qPCR to Discriminate between Live and Dead Legionella in Biofilm Samples
por: Taylor, Michael J, et al.
Publicado: (2014)

The effects of removing dead bacteria by propidium monoazide on the profile of salivary microbiome
por: Ren, Qidi, et al.
Publicado: (2021)

THE TOXOPLASMA GONDII OOCYST FROM CAT FECES
por: Dubey, J. P., et al.
Publicado: (1970)

Dynamics of Toxoplasma gondii Oocyst Phagocytosis by Macrophages
por: Ndao, Omar, et al.
Publicado: (2020)

Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide
por: Àlvarez, Gerard, et al.
Publicado: (2013)

Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens
por: Kim, Young Jin, et al.
Publicado: (2014)

Persistence of Legionella in Routinely Disinfected Heater-Cooler Units and Heater Units assessed by Propidium Monoazide qPCR
por: Ditommaso, Savina, et al.
Publicado: (2020)

Enumeration of Viable Non-Culturable Vibrio cholerae Using Droplet Digital PCR Combined With Propidium Monoazide Treatment
por: Zhao, Shuo, et al.
Publicado: (2021)

Two-Round Treatment With Propidium Monoazide Completely Inhibits the Detection of Dead Campylobacter spp. Cells by Quantitative PCR
por: Okada, Ayaka, et al.
Publicado: (2022)

Research Note: Detection of Campylobacter spp. in chicken meat using culture methods and quantitative PCR with propidium monoazide
por: Okada, Ayaka, et al.
Publicado: (2023)

Rapid Detection and Quantification of Viable Cells of Pectobacterium brasiliense Using Propidium Monoazide Combined with Real-Time PCR
por: Li, Junhui, et al.
Publicado: (2023)

Optimization of Propidium Monoazide qPCR (Viability-qPCR) to Quantify the Killing by the Gardnerella-Specific Endolysin PM-477, Directly in Vaginal Samples from Women with Bacterial Vaginosis
por: Latka, Agnieszka, et al.
Publicado: (2022)

Applicability of propidium monoazide (PMA) for discrimination between living and dead phytoplankton cells
por: Joo, Sungbae, et al.
Publicado: (2019)

Toxoplasma gondii Oocyst–specific Antibodies and Source of Infection
por: Muñoz-Zanzi, Claudia A., et al.
Publicado: (2010)

Monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qPCR combined with propidium monoazide
por: Yasunaga, Ai, et al.
Publicado: (2013)

A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic E. coli In Vitro and In Vivo
por: Lee, Albert S., et al.
Publicado: (2022)

An Improved Method for Quantification of Viable Fusarium Cells in Infected Soil Products by Propidium Monoazide Coupled with Real-Time PCR
por: Chen, Lida, et al.
Publicado: (2022)

Absolute quantification of viable Vibrio cholerae in seawater samples using multiplex droplet digital PCR combined with propidium monoazide
por: Yang, Jinsong, et al.
Publicado: (2023)

Structure, composition, and roles of the Toxoplasma gondii oocyst and sporocyst walls
por: Freppel, Wesley, et al.
Publicado: (2018)

Excretion of Toxoplasma gondii oocysts from Feral Cats in Korea
por: Ahn, Kyu-Sung, et al.
Publicado: (2019)

Detection of Toxoplasma gondii oocysts in fresh vegetables and berry fruits
por: Marques, Cláudia S., et al.
Publicado: (2020)

Environmental transmission of Toxoplasma gondii: Oocysts in water, soil and food
por: Shapiro, Karen, et al.
Publicado: (2019)

A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of Viable Burkholderia cepacia Complex in Nuclease-Free Water and Antiseptics
por: Daddy Gaoh, Soumana, et al.
Publicado: (2022)

Development of a sensitive and specific qPCR assay in conjunction with propidium monoazide for enhanced detection of live Salmonella spp. in food
por: Li, Baoguang, et al.
Publicado: (2013)

Propidium Monoazide Integrated with qPCR Enables the Detection and Enumeration of Infectious Enteric RNA and DNA Viruses in Clam and Fermented Sausages
por: Quijada, Narciso M., et al.
Publicado: (2016)

An Assay Combining Droplet Digital PCR With Propidium Monoazide Treatment for the Accurate Detection of Live Cells of Vibrio vulnificus in Plasma Samples
por: Hu, Ling, et al.
Publicado: (2022)

A new method for the rapid detection of the antibacterial and bacteriostatic activity of disinfectants based on Propidium Monoazide combined with real-time PCR
por: Liu, Yanrong, et al.
Publicado: (2022)

Propidium monoazide (PMA) and ethidium bromide monoazide (EMA) improve DNA array and high-throughput sequencing of porcine reproductive and respiratory syndrome virus identification
por: Bellehumeur, Christian, et al.
Publicado: (2015)

The antibacterial efficacy of silver diamine fluoride (SDF) is not modulated by potassium iodide (KI) supplements: A study on in-situ plaque biofilms using viability real-time PCR with propidium monoazide
por: Abdullah, Nizam, et al.
Publicado: (2020)

Rapid and Accurate Detection of Bacteriophage Activity against Escherichia coli O157:H7 by Propidium Monoazide Real-Time PCR
por: Liu, Hui, et al.
Publicado: (2014)

Propidium monoazide pretreatment on a 3D-printed microfluidic device for efficient PCR determination of live versus dead’microbial cells†
por: Zhu, Yanzhe, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_f9s89766unrjq2nlou4a5jesm4