Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication

Quorum sensing (QS) signals are widely utilized by bacteria to regulate biological functions in response to cell population density. Previous studies have demonstrated that Ralstonia solanacearum employs two different types of QS systems. We report here that anthranilic acid controls important biolo...

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Autores principales: Song, Shihao, Yin, Wenfang, Sun, Xiuyun, Cui, Binbin, Huang, Lei, Li, Peng, Yang, Liang, Zhou, Jianuan, Deng, Yinyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608240/
https://www.ncbi.nlm.nih.gov/pubmed/32457502
http://dx.doi.org/10.1038/s41396-020-0682-7
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author Song, Shihao
Yin, Wenfang
Sun, Xiuyun
Cui, Binbin
Huang, Lei
Li, Peng
Yang, Liang
Zhou, Jianuan
Deng, Yinyue
author_facet Song, Shihao
Yin, Wenfang
Sun, Xiuyun
Cui, Binbin
Huang, Lei
Li, Peng
Yang, Liang
Zhou, Jianuan
Deng, Yinyue
author_sort Song, Shihao
collection PubMed
description Quorum sensing (QS) signals are widely utilized by bacteria to regulate biological functions in response to cell population density. Previous studies have demonstrated that Ralstonia solanacearum employs two different types of QS systems. We report here that anthranilic acid controls important biological functions and the production of QS signals in R. solanacearum. It was demonstrated that the biosynthesis of anthranilic acid is mainly performed by TrpEG. The accumulation of anthranilic acid and the transcription of trpEG occur in a cell density-dependent manner in R. solanacearum. Both the anthranilic acid and TrpEG homologues are conserved in various bacterial species. Moreover, we show that Sporisorium scitamineum sexual mating and hypha formation are strongly inhibited by the addition of exogenous anthranilic acid. Our results suggest that anthranilic acid is important for the physiology of bacteria in addition to its role in inter-kingdom communication.
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spelling pubmed-76082402020-11-05 Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication Song, Shihao Yin, Wenfang Sun, Xiuyun Cui, Binbin Huang, Lei Li, Peng Yang, Liang Zhou, Jianuan Deng, Yinyue ISME J Article Quorum sensing (QS) signals are widely utilized by bacteria to regulate biological functions in response to cell population density. Previous studies have demonstrated that Ralstonia solanacearum employs two different types of QS systems. We report here that anthranilic acid controls important biological functions and the production of QS signals in R. solanacearum. It was demonstrated that the biosynthesis of anthranilic acid is mainly performed by TrpEG. The accumulation of anthranilic acid and the transcription of trpEG occur in a cell density-dependent manner in R. solanacearum. Both the anthranilic acid and TrpEG homologues are conserved in various bacterial species. Moreover, we show that Sporisorium scitamineum sexual mating and hypha formation are strongly inhibited by the addition of exogenous anthranilic acid. Our results suggest that anthranilic acid is important for the physiology of bacteria in addition to its role in inter-kingdom communication. Nature Publishing Group UK 2020-05-26 2020-09 /pmc/articles/PMC7608240/ /pubmed/32457502 http://dx.doi.org/10.1038/s41396-020-0682-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Song, Shihao
Yin, Wenfang
Sun, Xiuyun
Cui, Binbin
Huang, Lei
Li, Peng
Yang, Liang
Zhou, Jianuan
Deng, Yinyue
Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication
title Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication
title_full Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication
title_fullStr Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication
title_full_unstemmed Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication
title_short Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication
title_sort anthranilic acid from ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608240/
https://www.ncbi.nlm.nih.gov/pubmed/32457502
http://dx.doi.org/10.1038/s41396-020-0682-7
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