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Ethylene signaling mediates host invasion by parasitic plants

Parasitic plants form a specialized organ, a haustorium, to invade host tissues and acquire water and nutrients. To understand the molecular mechanism of haustorium development, we performed a forward genetics screening to isolate mutants exhibiting haustorial defects in the model parasitic plant Ph...

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Autores principales: Cui, Songkui, Kubota, Tomoya, Nishiyama, Tomoaki, Ishida, Juliane K., Shigenobu, Shuji, Shibata, Tomoko F., Toyoda, Atsushi, Hasebe, Mitsuyasu, Shirasu, Ken, Yoshida, Satoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608805/
https://www.ncbi.nlm.nih.gov/pubmed/33115743
http://dx.doi.org/10.1126/sciadv.abc2385
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author Cui, Songkui
Kubota, Tomoya
Nishiyama, Tomoaki
Ishida, Juliane K.
Shigenobu, Shuji
Shibata, Tomoko F.
Toyoda, Atsushi
Hasebe, Mitsuyasu
Shirasu, Ken
Yoshida, Satoko
author_facet Cui, Songkui
Kubota, Tomoya
Nishiyama, Tomoaki
Ishida, Juliane K.
Shigenobu, Shuji
Shibata, Tomoko F.
Toyoda, Atsushi
Hasebe, Mitsuyasu
Shirasu, Ken
Yoshida, Satoko
author_sort Cui, Songkui
collection PubMed
description Parasitic plants form a specialized organ, a haustorium, to invade host tissues and acquire water and nutrients. To understand the molecular mechanism of haustorium development, we performed a forward genetics screening to isolate mutants exhibiting haustorial defects in the model parasitic plant Phtheirospermum japonicum. We isolated two mutants that show prolonged and sometimes aberrant meristematic activity in the haustorium apex, resulting in severe defects on host invasion. Whole-genome sequencing revealed that the two mutants respectively have point mutations in homologs of ETHYLENE RESPONSE 1 (ETR1) and ETHYLENE INSENSITIVE 2 (EIN2), signaling components in response to the gaseous phytohormone ethylene. Application of the ethylene signaling inhibitors also caused similar haustorial defects, indicating that ethylene signaling regulates cell proliferation and differentiation of parasite cells. Genetic disruption of host ethylene production also perturbs parasite invasion. We propose that parasitic plants use ethylene as a signal to invade host roots.
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spelling pubmed-76088052020-11-13 Ethylene signaling mediates host invasion by parasitic plants Cui, Songkui Kubota, Tomoya Nishiyama, Tomoaki Ishida, Juliane K. Shigenobu, Shuji Shibata, Tomoko F. Toyoda, Atsushi Hasebe, Mitsuyasu Shirasu, Ken Yoshida, Satoko Sci Adv Research Articles Parasitic plants form a specialized organ, a haustorium, to invade host tissues and acquire water and nutrients. To understand the molecular mechanism of haustorium development, we performed a forward genetics screening to isolate mutants exhibiting haustorial defects in the model parasitic plant Phtheirospermum japonicum. We isolated two mutants that show prolonged and sometimes aberrant meristematic activity in the haustorium apex, resulting in severe defects on host invasion. Whole-genome sequencing revealed that the two mutants respectively have point mutations in homologs of ETHYLENE RESPONSE 1 (ETR1) and ETHYLENE INSENSITIVE 2 (EIN2), signaling components in response to the gaseous phytohormone ethylene. Application of the ethylene signaling inhibitors also caused similar haustorial defects, indicating that ethylene signaling regulates cell proliferation and differentiation of parasite cells. Genetic disruption of host ethylene production also perturbs parasite invasion. We propose that parasitic plants use ethylene as a signal to invade host roots. American Association for the Advancement of Science 2020-10-28 /pmc/articles/PMC7608805/ /pubmed/33115743 http://dx.doi.org/10.1126/sciadv.abc2385 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Cui, Songkui
Kubota, Tomoya
Nishiyama, Tomoaki
Ishida, Juliane K.
Shigenobu, Shuji
Shibata, Tomoko F.
Toyoda, Atsushi
Hasebe, Mitsuyasu
Shirasu, Ken
Yoshida, Satoko
Ethylene signaling mediates host invasion by parasitic plants
title Ethylene signaling mediates host invasion by parasitic plants
title_full Ethylene signaling mediates host invasion by parasitic plants
title_fullStr Ethylene signaling mediates host invasion by parasitic plants
title_full_unstemmed Ethylene signaling mediates host invasion by parasitic plants
title_short Ethylene signaling mediates host invasion by parasitic plants
title_sort ethylene signaling mediates host invasion by parasitic plants
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608805/
https://www.ncbi.nlm.nih.gov/pubmed/33115743
http://dx.doi.org/10.1126/sciadv.abc2385
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