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Hyperspectral infrared microscopy with visible light
Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint across different regions of a sample. Challenges associated w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608807/ https://www.ncbi.nlm.nih.gov/pubmed/33127685 http://dx.doi.org/10.1126/sciadv.abd0460 |
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author | Paterova, Anna V. Maniam, Sivakumar M. Yang, Hongzhi Grenci, Gianluca Krivitsky, Leonid A. |
author_facet | Paterova, Anna V. Maniam, Sivakumar M. Yang, Hongzhi Grenci, Gianluca Krivitsky, Leonid A. |
author_sort | Paterova, Anna V. |
collection | PubMed |
description | Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint across different regions of a sample. Challenges associated with low efficiency and high cost of IR light sources and detector arrays have limited its broad adoption. Here, we introduce a new approach to IR hyperspectral microscopy, where the IR spectral map is obtained with off-the-shelf components built for visible light. The method is based on the nonlinear interference of correlated photons generated via parametric down-conversion. In this proof-of-concept we demonstrate the chemical mapping of a patterned sample, where different areas have distinctive IR spectroscopic fingerprints. The method provides a wide field of view, fast readout, and negligible heat delivered to the sample, which opens prospects for its further development for applications in material and biological studies. |
format | Online Article Text |
id | pubmed-7608807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76088072020-11-13 Hyperspectral infrared microscopy with visible light Paterova, Anna V. Maniam, Sivakumar M. Yang, Hongzhi Grenci, Gianluca Krivitsky, Leonid A. Sci Adv Research Articles Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint across different regions of a sample. Challenges associated with low efficiency and high cost of IR light sources and detector arrays have limited its broad adoption. Here, we introduce a new approach to IR hyperspectral microscopy, where the IR spectral map is obtained with off-the-shelf components built for visible light. The method is based on the nonlinear interference of correlated photons generated via parametric down-conversion. In this proof-of-concept we demonstrate the chemical mapping of a patterned sample, where different areas have distinctive IR spectroscopic fingerprints. The method provides a wide field of view, fast readout, and negligible heat delivered to the sample, which opens prospects for its further development for applications in material and biological studies. American Association for the Advancement of Science 2020-10-30 /pmc/articles/PMC7608807/ /pubmed/33127685 http://dx.doi.org/10.1126/sciadv.abd0460 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Paterova, Anna V. Maniam, Sivakumar M. Yang, Hongzhi Grenci, Gianluca Krivitsky, Leonid A. Hyperspectral infrared microscopy with visible light |
title | Hyperspectral infrared microscopy with visible light |
title_full | Hyperspectral infrared microscopy with visible light |
title_fullStr | Hyperspectral infrared microscopy with visible light |
title_full_unstemmed | Hyperspectral infrared microscopy with visible light |
title_short | Hyperspectral infrared microscopy with visible light |
title_sort | hyperspectral infrared microscopy with visible light |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608807/ https://www.ncbi.nlm.nih.gov/pubmed/33127685 http://dx.doi.org/10.1126/sciadv.abd0460 |
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