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Hyperspectral infrared microscopy with visible light

Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint across different regions of a sample. Challenges associated w...

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Autores principales: Paterova, Anna V., Maniam, Sivakumar M., Yang, Hongzhi, Grenci, Gianluca, Krivitsky, Leonid A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608807/
https://www.ncbi.nlm.nih.gov/pubmed/33127685
http://dx.doi.org/10.1126/sciadv.abd0460
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author Paterova, Anna V.
Maniam, Sivakumar M.
Yang, Hongzhi
Grenci, Gianluca
Krivitsky, Leonid A.
author_facet Paterova, Anna V.
Maniam, Sivakumar M.
Yang, Hongzhi
Grenci, Gianluca
Krivitsky, Leonid A.
author_sort Paterova, Anna V.
collection PubMed
description Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint across different regions of a sample. Challenges associated with low efficiency and high cost of IR light sources and detector arrays have limited its broad adoption. Here, we introduce a new approach to IR hyperspectral microscopy, where the IR spectral map is obtained with off-the-shelf components built for visible light. The method is based on the nonlinear interference of correlated photons generated via parametric down-conversion. In this proof-of-concept we demonstrate the chemical mapping of a patterned sample, where different areas have distinctive IR spectroscopic fingerprints. The method provides a wide field of view, fast readout, and negligible heat delivered to the sample, which opens prospects for its further development for applications in material and biological studies.
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spelling pubmed-76088072020-11-13 Hyperspectral infrared microscopy with visible light Paterova, Anna V. Maniam, Sivakumar M. Yang, Hongzhi Grenci, Gianluca Krivitsky, Leonid A. Sci Adv Research Articles Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint across different regions of a sample. Challenges associated with low efficiency and high cost of IR light sources and detector arrays have limited its broad adoption. Here, we introduce a new approach to IR hyperspectral microscopy, where the IR spectral map is obtained with off-the-shelf components built for visible light. The method is based on the nonlinear interference of correlated photons generated via parametric down-conversion. In this proof-of-concept we demonstrate the chemical mapping of a patterned sample, where different areas have distinctive IR spectroscopic fingerprints. The method provides a wide field of view, fast readout, and negligible heat delivered to the sample, which opens prospects for its further development for applications in material and biological studies. American Association for the Advancement of Science 2020-10-30 /pmc/articles/PMC7608807/ /pubmed/33127685 http://dx.doi.org/10.1126/sciadv.abd0460 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Paterova, Anna V.
Maniam, Sivakumar M.
Yang, Hongzhi
Grenci, Gianluca
Krivitsky, Leonid A.
Hyperspectral infrared microscopy with visible light
title Hyperspectral infrared microscopy with visible light
title_full Hyperspectral infrared microscopy with visible light
title_fullStr Hyperspectral infrared microscopy with visible light
title_full_unstemmed Hyperspectral infrared microscopy with visible light
title_short Hyperspectral infrared microscopy with visible light
title_sort hyperspectral infrared microscopy with visible light
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608807/
https://www.ncbi.nlm.nih.gov/pubmed/33127685
http://dx.doi.org/10.1126/sciadv.abd0460
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