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Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum

Protozoan parasites of the Cryptosporidium genus cause severe cryptosporidiosis in newborn lambs. However, asymptomatic infections also occur frequently in lambs and ewes. In sheep, the most commonly detected Cryptosporidium species are C. ubiquitum, C. xiaoi and C. parvum. Due to a lack of relevant...

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Autores principales: Bordes, Léa, Houert, Pauline, Costa, Damien, Favennec, Loïc, Vial-Novella, Corinne, Fidelle, Francis, Grisez, Christelle, Prévot, Françoise, Jacquiet, Philippe, Razakandrainibe, Romy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608980/
https://www.ncbi.nlm.nih.gov/pubmed/33141660
http://dx.doi.org/10.1051/parasite/2020054
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author Bordes, Léa
Houert, Pauline
Costa, Damien
Favennec, Loïc
Vial-Novella, Corinne
Fidelle, Francis
Grisez, Christelle
Prévot, Françoise
Jacquiet, Philippe
Razakandrainibe, Romy
author_facet Bordes, Léa
Houert, Pauline
Costa, Damien
Favennec, Loïc
Vial-Novella, Corinne
Fidelle, Francis
Grisez, Christelle
Prévot, Françoise
Jacquiet, Philippe
Razakandrainibe, Romy
author_sort Bordes, Léa
collection PubMed
description Protozoan parasites of the Cryptosporidium genus cause severe cryptosporidiosis in newborn lambs. However, asymptomatic infections also occur frequently in lambs and ewes. In sheep, the most commonly detected Cryptosporidium species are C. ubiquitum, C. xiaoi and C. parvum. Due to a lack of relevant information about such infections in France, we investigated the situation on five dairy sheep farms in the Pyrénées-Atlantiques Department in south-western France in December 2017. Individual fecal samples were collected from 79 female lambs (5–17 days old) and their mothers (72 ewes). Oocysts were screened using Heine staining before and after Bailenger concentrations. Cryptosporidium species identification and genotyping were performed using real-time PCR and gp60 gene sequencing. No cases of clinical cryptosporidiosis were observed in the 79 lambs. Microscopically, Cryptosporidium spp. oocysts were observed in only one lamb on one farm (prevalence 1.3%) and one ewe on another farm (prevalence 1.4%). By contrast, Cryptosporidium spp. DNA was detected in 17 ewes (prevalence ranging from 10.5% to 50% depending on the farm) and in 36 lambs (prevalence ranging from 0% to 77.8% depending on the farm). Only zoonotic Cryptosporidium parvum IId and IIa genotypes were identified when genotyping was possible. Cryptosporidium ubiquitum and C. xiaoi were detected on one and three farms, respectively. We conclude that healthy young lambs and their mothers during the peripartum period could be a source of environmental contamination with oocysts.
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spelling pubmed-76089802020-11-09 Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum Bordes, Léa Houert, Pauline Costa, Damien Favennec, Loïc Vial-Novella, Corinne Fidelle, Francis Grisez, Christelle Prévot, Françoise Jacquiet, Philippe Razakandrainibe, Romy Parasite Research Article Protozoan parasites of the Cryptosporidium genus cause severe cryptosporidiosis in newborn lambs. However, asymptomatic infections also occur frequently in lambs and ewes. In sheep, the most commonly detected Cryptosporidium species are C. ubiquitum, C. xiaoi and C. parvum. Due to a lack of relevant information about such infections in France, we investigated the situation on five dairy sheep farms in the Pyrénées-Atlantiques Department in south-western France in December 2017. Individual fecal samples were collected from 79 female lambs (5–17 days old) and their mothers (72 ewes). Oocysts were screened using Heine staining before and after Bailenger concentrations. Cryptosporidium species identification and genotyping were performed using real-time PCR and gp60 gene sequencing. No cases of clinical cryptosporidiosis were observed in the 79 lambs. Microscopically, Cryptosporidium spp. oocysts were observed in only one lamb on one farm (prevalence 1.3%) and one ewe on another farm (prevalence 1.4%). By contrast, Cryptosporidium spp. DNA was detected in 17 ewes (prevalence ranging from 10.5% to 50% depending on the farm) and in 36 lambs (prevalence ranging from 0% to 77.8% depending on the farm). Only zoonotic Cryptosporidium parvum IId and IIa genotypes were identified when genotyping was possible. Cryptosporidium ubiquitum and C. xiaoi were detected on one and three farms, respectively. We conclude that healthy young lambs and their mothers during the peripartum period could be a source of environmental contamination with oocysts. EDP Sciences 2020-11-03 /pmc/articles/PMC7608980/ /pubmed/33141660 http://dx.doi.org/10.1051/parasite/2020054 Text en © L. Bordes et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bordes, Léa
Houert, Pauline
Costa, Damien
Favennec, Loïc
Vial-Novella, Corinne
Fidelle, Francis
Grisez, Christelle
Prévot, Françoise
Jacquiet, Philippe
Razakandrainibe, Romy
Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum
title Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum
title_full Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum
title_fullStr Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum
title_full_unstemmed Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum
title_short Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum
title_sort asymptomatic cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of cryptosporidium parvum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608980/
https://www.ncbi.nlm.nih.gov/pubmed/33141660
http://dx.doi.org/10.1051/parasite/2020054
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