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CRISPR-based gene expression control for synthetic gene circuits
Synthetic gene circuits allow us to govern cell behavior in a programmable manner, which is central to almost any application aiming to harness engineered living cells for user-defined tasks. Transcription factors (TFs) constitute the ‘classic’ tool for synthetic circuit construction but some of the...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609024/ https://www.ncbi.nlm.nih.gov/pubmed/32964920 http://dx.doi.org/10.1042/BST20200020 |
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author | Santos-Moreno, Javier Schaerli, Yolanda |
author_facet | Santos-Moreno, Javier Schaerli, Yolanda |
author_sort | Santos-Moreno, Javier |
collection | PubMed |
description | Synthetic gene circuits allow us to govern cell behavior in a programmable manner, which is central to almost any application aiming to harness engineered living cells for user-defined tasks. Transcription factors (TFs) constitute the ‘classic’ tool for synthetic circuit construction but some of their inherent constraints, such as insufficient modularity, orthogonality and programmability, limit progress in such forward-engineering endeavors. Here we review how CRISPR (clustered regularly interspaced short palindromic repeats) technology offers new and powerful possibilities for synthetic circuit design. CRISPR systems offer superior characteristics over TFs in many aspects relevant to a modular, predictable and standardized circuit design. Thus, the choice of CRISPR technology as a framework for synthetic circuit design constitutes a valid alternative to complement or replace TFs in synthetic circuits and promises the realization of more ambitious designs. |
format | Online Article Text |
id | pubmed-7609024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76090242020-11-06 CRISPR-based gene expression control for synthetic gene circuits Santos-Moreno, Javier Schaerli, Yolanda Biochem Soc Trans Review Articles Synthetic gene circuits allow us to govern cell behavior in a programmable manner, which is central to almost any application aiming to harness engineered living cells for user-defined tasks. Transcription factors (TFs) constitute the ‘classic’ tool for synthetic circuit construction but some of their inherent constraints, such as insufficient modularity, orthogonality and programmability, limit progress in such forward-engineering endeavors. Here we review how CRISPR (clustered regularly interspaced short palindromic repeats) technology offers new and powerful possibilities for synthetic circuit design. CRISPR systems offer superior characteristics over TFs in many aspects relevant to a modular, predictable and standardized circuit design. Thus, the choice of CRISPR technology as a framework for synthetic circuit design constitutes a valid alternative to complement or replace TFs in synthetic circuits and promises the realization of more ambitious designs. Portland Press Ltd. 2020-10-30 2020-09-23 /pmc/articles/PMC7609024/ /pubmed/32964920 http://dx.doi.org/10.1042/BST20200020 Text en © 2020 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Articles Santos-Moreno, Javier Schaerli, Yolanda CRISPR-based gene expression control for synthetic gene circuits |
title | CRISPR-based gene expression control for synthetic gene circuits |
title_full | CRISPR-based gene expression control for synthetic gene circuits |
title_fullStr | CRISPR-based gene expression control for synthetic gene circuits |
title_full_unstemmed | CRISPR-based gene expression control for synthetic gene circuits |
title_short | CRISPR-based gene expression control for synthetic gene circuits |
title_sort | crispr-based gene expression control for synthetic gene circuits |
topic | Review Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609024/ https://www.ncbi.nlm.nih.gov/pubmed/32964920 http://dx.doi.org/10.1042/BST20200020 |
work_keys_str_mv | AT santosmorenojavier crisprbasedgeneexpressioncontrolforsyntheticgenecircuits AT schaerliyolanda crisprbasedgeneexpressioncontrolforsyntheticgenecircuits |