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Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo
Cell behaviour and function is determined through the interactions of a multitude of molecules working in concert. To observe these molecular dynamics, biophysical studies have been developed that track single interactions. Fluorescence correlation spectroscopy (FCS) is an optical biophysical techni...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609432/ https://www.ncbi.nlm.nih.gov/pubmed/33067656 http://dx.doi.org/10.1007/s00418-020-01930-5 |
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author | Dawes, Michael L. Soeller, Christian Scholpp, Steffen |
author_facet | Dawes, Michael L. Soeller, Christian Scholpp, Steffen |
author_sort | Dawes, Michael L. |
collection | PubMed |
description | Cell behaviour and function is determined through the interactions of a multitude of molecules working in concert. To observe these molecular dynamics, biophysical studies have been developed that track single interactions. Fluorescence correlation spectroscopy (FCS) is an optical biophysical technique that non-invasively resolves single molecules through recording the signal intensity at the femtolitre scale. However, recording the behaviour of these biomolecules using in vitro-based assays often fails to recapitulate the full range of variables in vivo that directly confer dynamics. Therefore, there has been an increasing interest in observing the state of these biomolecules within living organisms such as the zebrafish Danio rerio. In this review, we explore the advancements of FCS within the zebrafish and compare and contrast these findings to those found in vitro. |
format | Online Article Text |
id | pubmed-7609432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-76094322020-11-10 Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo Dawes, Michael L. Soeller, Christian Scholpp, Steffen Histochem Cell Biol Review Cell behaviour and function is determined through the interactions of a multitude of molecules working in concert. To observe these molecular dynamics, biophysical studies have been developed that track single interactions. Fluorescence correlation spectroscopy (FCS) is an optical biophysical technique that non-invasively resolves single molecules through recording the signal intensity at the femtolitre scale. However, recording the behaviour of these biomolecules using in vitro-based assays often fails to recapitulate the full range of variables in vivo that directly confer dynamics. Therefore, there has been an increasing interest in observing the state of these biomolecules within living organisms such as the zebrafish Danio rerio. In this review, we explore the advancements of FCS within the zebrafish and compare and contrast these findings to those found in vitro. Springer Berlin Heidelberg 2020-10-16 2020 /pmc/articles/PMC7609432/ /pubmed/33067656 http://dx.doi.org/10.1007/s00418-020-01930-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Review Dawes, Michael L. Soeller, Christian Scholpp, Steffen Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo |
title | Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo |
title_full | Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo |
title_fullStr | Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo |
title_full_unstemmed | Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo |
title_short | Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo |
title_sort | studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609432/ https://www.ncbi.nlm.nih.gov/pubmed/33067656 http://dx.doi.org/10.1007/s00418-020-01930-5 |
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