Cargando…
Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease
A nutritional intervention, exclusive enteral nutrition (EEN) can induce remission in patients with pediatric Crohn’s disease (CD). We characterized changes in the fecal microbiota and metabolome to identify the mechanism of EEN. Feces of 43 children were collected prior, during and after EEN. Micro...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609694/ https://www.ncbi.nlm.nih.gov/pubmed/33144591 http://dx.doi.org/10.1038/s41598-020-75306-z |
_version_ | 1783605059048701952 |
---|---|
author | Diederen, Kay Li, Jia V. Donachie, Gillian E. de Meij, Tim G. de Waart, Dirk R. Hakvoort, Theodorus B. M. Kindermann, Angelika Wagner, Josef Auyeung, Victoria te Velde, Anje A. Heinsbroek, Sigrid E. M. Benninga, Marc A. Kinross, James Walker, Alan W. de Jonge, Wouter J. Seppen, Jurgen |
author_facet | Diederen, Kay Li, Jia V. Donachie, Gillian E. de Meij, Tim G. de Waart, Dirk R. Hakvoort, Theodorus B. M. Kindermann, Angelika Wagner, Josef Auyeung, Victoria te Velde, Anje A. Heinsbroek, Sigrid E. M. Benninga, Marc A. Kinross, James Walker, Alan W. de Jonge, Wouter J. Seppen, Jurgen |
author_sort | Diederen, Kay |
collection | PubMed |
description | A nutritional intervention, exclusive enteral nutrition (EEN) can induce remission in patients with pediatric Crohn’s disease (CD). We characterized changes in the fecal microbiota and metabolome to identify the mechanism of EEN. Feces of 43 children were collected prior, during and after EEN. Microbiota and metabolites were analyzed by 16S rRNA gene amplicon sequencing and NMR. Selected metabolites were evaluated in relevant model systems. Microbiota and metabolome of patients with CD and controls were different at all time points. Amino acids, primary bile salts, trimethylamine and cadaverine were elevated in patients with CD. Microbiota and metabolome differed between responders and non-responders prior to EEN. EEN decreased microbiota diversity and reduced amino acids, trimethylamine and cadaverine towards control levels. Patients with CD had reduced microbial metabolism of bile acids that partially normalized during EEN. Trimethylamine and cadaverine inhibited intestinal cell growth. TMA and cadaverine inhibited LPS-stimulated TNF-alpha and IL-6 secretion by primary human monocytes. A diet rich in free amino acids worsened inflammation in the DSS model of intestinal inflammation. Trimethylamine, cadaverine, bile salts and amino acids could play a role in the mechanism by which EEN induces remission. Prior to EEN, microbiota and metabolome are different between responders and non-responders. |
format | Online Article Text |
id | pubmed-7609694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76096942020-11-05 Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease Diederen, Kay Li, Jia V. Donachie, Gillian E. de Meij, Tim G. de Waart, Dirk R. Hakvoort, Theodorus B. M. Kindermann, Angelika Wagner, Josef Auyeung, Victoria te Velde, Anje A. Heinsbroek, Sigrid E. M. Benninga, Marc A. Kinross, James Walker, Alan W. de Jonge, Wouter J. Seppen, Jurgen Sci Rep Article A nutritional intervention, exclusive enteral nutrition (EEN) can induce remission in patients with pediatric Crohn’s disease (CD). We characterized changes in the fecal microbiota and metabolome to identify the mechanism of EEN. Feces of 43 children were collected prior, during and after EEN. Microbiota and metabolites were analyzed by 16S rRNA gene amplicon sequencing and NMR. Selected metabolites were evaluated in relevant model systems. Microbiota and metabolome of patients with CD and controls were different at all time points. Amino acids, primary bile salts, trimethylamine and cadaverine were elevated in patients with CD. Microbiota and metabolome differed between responders and non-responders prior to EEN. EEN decreased microbiota diversity and reduced amino acids, trimethylamine and cadaverine towards control levels. Patients with CD had reduced microbial metabolism of bile acids that partially normalized during EEN. Trimethylamine and cadaverine inhibited intestinal cell growth. TMA and cadaverine inhibited LPS-stimulated TNF-alpha and IL-6 secretion by primary human monocytes. A diet rich in free amino acids worsened inflammation in the DSS model of intestinal inflammation. Trimethylamine, cadaverine, bile salts and amino acids could play a role in the mechanism by which EEN induces remission. Prior to EEN, microbiota and metabolome are different between responders and non-responders. Nature Publishing Group UK 2020-11-03 /pmc/articles/PMC7609694/ /pubmed/33144591 http://dx.doi.org/10.1038/s41598-020-75306-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Diederen, Kay Li, Jia V. Donachie, Gillian E. de Meij, Tim G. de Waart, Dirk R. Hakvoort, Theodorus B. M. Kindermann, Angelika Wagner, Josef Auyeung, Victoria te Velde, Anje A. Heinsbroek, Sigrid E. M. Benninga, Marc A. Kinross, James Walker, Alan W. de Jonge, Wouter J. Seppen, Jurgen Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease |
title | Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease |
title_full | Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease |
title_fullStr | Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease |
title_full_unstemmed | Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease |
title_short | Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease |
title_sort | exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with crohn’s disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609694/ https://www.ncbi.nlm.nih.gov/pubmed/33144591 http://dx.doi.org/10.1038/s41598-020-75306-z |
work_keys_str_mv | AT diederenkay exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT lijiav exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT donachiegilliane exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT demeijtimg exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT dewaartdirkr exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT hakvoorttheodorusbm exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT kindermannangelika exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT wagnerjosef exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT auyeungvictoria exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT teveldeanjea exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT heinsbroeksigridem exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT benningamarca exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT kinrossjames exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT walkeralanw exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT dejongewouterj exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease AT seppenjurgen exclusiveenteralnutritionmediatesgutmicrobialandmetabolicchangesthatareassociatedwithremissioninchildrenwithcrohnsdisease |