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Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons

During embryonic development, symmetric ectodermal thickenings [olfactory placodes (OP)] give rise to several cell types that comprise the olfactory system, such as those that form the terminal nerve ganglion (TN), gonadotropin releasing hormone-1 neurons (GnRH-1ns), and other migratory neurons in r...

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Autores principales: Taroc, Ed Zandro M., Katreddi, Raghu Ram, Forni, Paolo E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609815/
https://www.ncbi.nlm.nih.gov/pubmed/33192618
http://dx.doi.org/10.3389/fphys.2020.601923
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author Taroc, Ed Zandro M.
Katreddi, Raghu Ram
Forni, Paolo E.
author_facet Taroc, Ed Zandro M.
Katreddi, Raghu Ram
Forni, Paolo E.
author_sort Taroc, Ed Zandro M.
collection PubMed
description During embryonic development, symmetric ectodermal thickenings [olfactory placodes (OP)] give rise to several cell types that comprise the olfactory system, such as those that form the terminal nerve ganglion (TN), gonadotropin releasing hormone-1 neurons (GnRH-1ns), and other migratory neurons in rodents. Even though the genetic heterogeneity among these cell types is documented, unidentified cell populations arising from the OP remain. One candidate to identify placodal derived neurons in the developing nasal area is the transcription factor Isl1, which was recently identified in GnRH-3 neurons of the terminal nerve in fish, as well as expression in neurons of the nasal migratory mass (MM). Here, we analyzed the Isl1 genetic lineage in chemosensory neuronal populations in the nasal area and migratory GnRH-1ns in mice using in situ hybridization, immunolabeling a Tamoxifen inducible Isl1Cre(ERT) and a constitutive Isl1(Cre) knock-in mouse lines. In addition, we also performed conditional Isl1 ablation in developing GnRH neurons. We found Isl1 lineage across non-sensory cells of the respiratory epithelium and sustentacular cells of OE and VNO. We identified a population of transient embryonic Isl1 + neurons in the olfactory epithelium and sparse Isl1 + neurons in postnatal VNO. Isl1 is expressed in almost all GnRH neurons and in approximately half of the other neuron populations in the MM. However, Isl1 conditional ablation alone does not significantly compromise GnRH-1 neuronal migration or GnRH-1 expression, suggesting compensatory mechanisms. Further studies will elucidate the functional and mechanistic role of Isl1 in development of migratory endocrine neurons.
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spelling pubmed-76098152020-11-13 Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons Taroc, Ed Zandro M. Katreddi, Raghu Ram Forni, Paolo E. Front Physiol Physiology During embryonic development, symmetric ectodermal thickenings [olfactory placodes (OP)] give rise to several cell types that comprise the olfactory system, such as those that form the terminal nerve ganglion (TN), gonadotropin releasing hormone-1 neurons (GnRH-1ns), and other migratory neurons in rodents. Even though the genetic heterogeneity among these cell types is documented, unidentified cell populations arising from the OP remain. One candidate to identify placodal derived neurons in the developing nasal area is the transcription factor Isl1, which was recently identified in GnRH-3 neurons of the terminal nerve in fish, as well as expression in neurons of the nasal migratory mass (MM). Here, we analyzed the Isl1 genetic lineage in chemosensory neuronal populations in the nasal area and migratory GnRH-1ns in mice using in situ hybridization, immunolabeling a Tamoxifen inducible Isl1Cre(ERT) and a constitutive Isl1(Cre) knock-in mouse lines. In addition, we also performed conditional Isl1 ablation in developing GnRH neurons. We found Isl1 lineage across non-sensory cells of the respiratory epithelium and sustentacular cells of OE and VNO. We identified a population of transient embryonic Isl1 + neurons in the olfactory epithelium and sparse Isl1 + neurons in postnatal VNO. Isl1 is expressed in almost all GnRH neurons and in approximately half of the other neuron populations in the MM. However, Isl1 conditional ablation alone does not significantly compromise GnRH-1 neuronal migration or GnRH-1 expression, suggesting compensatory mechanisms. Further studies will elucidate the functional and mechanistic role of Isl1 in development of migratory endocrine neurons. Frontiers Media S.A. 2020-10-21 /pmc/articles/PMC7609815/ /pubmed/33192618 http://dx.doi.org/10.3389/fphys.2020.601923 Text en Copyright © 2020 Taroc, Katreddi and Forni. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Taroc, Ed Zandro M.
Katreddi, Raghu Ram
Forni, Paolo E.
Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons
title Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons
title_full Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons
title_fullStr Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons
title_full_unstemmed Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons
title_short Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons
title_sort identifying isl1 genetic lineage in the developing olfactory system and in gnrh-1 neurons
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609815/
https://www.ncbi.nlm.nih.gov/pubmed/33192618
http://dx.doi.org/10.3389/fphys.2020.601923
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