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Lugol’s Iodine-Enhanced Micro-CT: A Potential 3-D Imaging Method for Detecting Tongue Squamous Cell Carcinoma Specimens in Surgery

BACKGROUND: A positive surgical margin (PSM) following oral cancer resection results in local recurrence and poor prognosis. Mono-block tumor specimens, especially from the tumor base, are difficult to evaluate. This inaccurate sampling ultimately leads to a false pathological diagnosis. Lugol’s iod...

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Autores principales: Xia, Cheng-Wan, Gan, Rong-lin, Pan, Jiong-ru, Hu, Shi-qi, Zhou, Qun-zhi, Chen, Shen, Zhang, Lei, Hu, Qin-Gang, Wang, Yu-Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609877/
https://www.ncbi.nlm.nih.gov/pubmed/33194607
http://dx.doi.org/10.3389/fonc.2020.550171
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author Xia, Cheng-Wan
Gan, Rong-lin
Pan, Jiong-ru
Hu, Shi-qi
Zhou, Qun-zhi
Chen, Shen
Zhang, Lei
Hu, Qin-Gang
Wang, Yu-Xin
author_facet Xia, Cheng-Wan
Gan, Rong-lin
Pan, Jiong-ru
Hu, Shi-qi
Zhou, Qun-zhi
Chen, Shen
Zhang, Lei
Hu, Qin-Gang
Wang, Yu-Xin
author_sort Xia, Cheng-Wan
collection PubMed
description BACKGROUND: A positive surgical margin (PSM) following oral cancer resection results in local recurrence and poor prognosis. Mono-block tumor specimens, especially from the tumor base, are difficult to evaluate. This inaccurate sampling ultimately leads to a false pathological diagnosis. Lugol’s iodine (I(2)-IK)-enhanced micro-CT is an emerging method to image tumor specimens. This study explores the feasibility of I(2)-IK-enhanced micro-CT to evaluate the surgical margin for tongue squamous cell carcinoma (TSCC) specimens and to further seek optimal staining parameters. METHODS: Rabbit tongue tissues and human TSCC samples were imaged via I(2)-IK-enhanced micro-CT. The optimal I(2)-IK concentration and staining time were determined before clinical application using tissue shrinkage, micro-CT image quality, and effect on pathological diagnosis as assessment criteria. Next, 6 TSCC specimens were used to verify the process feasibility of surgical margin imaging with the optimal parameters. Finally, the possible reason by which I(2)-IK could enhance micro-CT imaging was validated in vitro. RESULTS: I(2)-IK staining influenced specimen shrinkage, micro-CT image quality, and pathological image quality in a concentration- and time-dependent manner. After comprehensively considering these indicators, 3% I(2)-IK staining for 48 and 12 h were found to be optimal for rabbit tongue tissues and TSCC samples, respectively. This method could provide a detailed 3-D structure of TSCC samples compared with H&E sections. Moreover, tumor and normal tissues could be differentiated by their glycogen content, which has high affinity with I(2)-IK. CONCLUSIONS: I(2)-IK-enhanced micro-CT could, thus, indicate the tumor margin and assist pathological sampling in patients with TSCC postoperation.
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spelling pubmed-76098772020-11-13 Lugol’s Iodine-Enhanced Micro-CT: A Potential 3-D Imaging Method for Detecting Tongue Squamous Cell Carcinoma Specimens in Surgery Xia, Cheng-Wan Gan, Rong-lin Pan, Jiong-ru Hu, Shi-qi Zhou, Qun-zhi Chen, Shen Zhang, Lei Hu, Qin-Gang Wang, Yu-Xin Front Oncol Oncology BACKGROUND: A positive surgical margin (PSM) following oral cancer resection results in local recurrence and poor prognosis. Mono-block tumor specimens, especially from the tumor base, are difficult to evaluate. This inaccurate sampling ultimately leads to a false pathological diagnosis. Lugol’s iodine (I(2)-IK)-enhanced micro-CT is an emerging method to image tumor specimens. This study explores the feasibility of I(2)-IK-enhanced micro-CT to evaluate the surgical margin for tongue squamous cell carcinoma (TSCC) specimens and to further seek optimal staining parameters. METHODS: Rabbit tongue tissues and human TSCC samples were imaged via I(2)-IK-enhanced micro-CT. The optimal I(2)-IK concentration and staining time were determined before clinical application using tissue shrinkage, micro-CT image quality, and effect on pathological diagnosis as assessment criteria. Next, 6 TSCC specimens were used to verify the process feasibility of surgical margin imaging with the optimal parameters. Finally, the possible reason by which I(2)-IK could enhance micro-CT imaging was validated in vitro. RESULTS: I(2)-IK staining influenced specimen shrinkage, micro-CT image quality, and pathological image quality in a concentration- and time-dependent manner. After comprehensively considering these indicators, 3% I(2)-IK staining for 48 and 12 h were found to be optimal for rabbit tongue tissues and TSCC samples, respectively. This method could provide a detailed 3-D structure of TSCC samples compared with H&E sections. Moreover, tumor and normal tissues could be differentiated by their glycogen content, which has high affinity with I(2)-IK. CONCLUSIONS: I(2)-IK-enhanced micro-CT could, thus, indicate the tumor margin and assist pathological sampling in patients with TSCC postoperation. Frontiers Media S.A. 2020-10-21 /pmc/articles/PMC7609877/ /pubmed/33194607 http://dx.doi.org/10.3389/fonc.2020.550171 Text en Copyright © 2020 Xia, Gan, Pan, Hu, Zhou, Chen, Zhang, Hu and Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Xia, Cheng-Wan
Gan, Rong-lin
Pan, Jiong-ru
Hu, Shi-qi
Zhou, Qun-zhi
Chen, Shen
Zhang, Lei
Hu, Qin-Gang
Wang, Yu-Xin
Lugol’s Iodine-Enhanced Micro-CT: A Potential 3-D Imaging Method for Detecting Tongue Squamous Cell Carcinoma Specimens in Surgery
title Lugol’s Iodine-Enhanced Micro-CT: A Potential 3-D Imaging Method for Detecting Tongue Squamous Cell Carcinoma Specimens in Surgery
title_full Lugol’s Iodine-Enhanced Micro-CT: A Potential 3-D Imaging Method for Detecting Tongue Squamous Cell Carcinoma Specimens in Surgery
title_fullStr Lugol’s Iodine-Enhanced Micro-CT: A Potential 3-D Imaging Method for Detecting Tongue Squamous Cell Carcinoma Specimens in Surgery
title_full_unstemmed Lugol’s Iodine-Enhanced Micro-CT: A Potential 3-D Imaging Method for Detecting Tongue Squamous Cell Carcinoma Specimens in Surgery
title_short Lugol’s Iodine-Enhanced Micro-CT: A Potential 3-D Imaging Method for Detecting Tongue Squamous Cell Carcinoma Specimens in Surgery
title_sort lugol’s iodine-enhanced micro-ct: a potential 3-d imaging method for detecting tongue squamous cell carcinoma specimens in surgery
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609877/
https://www.ncbi.nlm.nih.gov/pubmed/33194607
http://dx.doi.org/10.3389/fonc.2020.550171
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