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Age-related alterations in fertilization-induced Ca(2+) oscillations depend on the genetic background of mouse oocytes(†)
Maternal aging affects various aspects of oocytes’ physiology, including the functionality of their nuclear apparatus and mitochondria. In the present paper, we wished to investigate whether advanced reproductive age impacts oocytes’ ability to generate proper Ca(2+) oscillations in response to mono...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609943/ https://www.ncbi.nlm.nih.gov/pubmed/32761132 http://dx.doi.org/10.1093/biolre/ioaa139 |
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author | Czajkowska, Katarzyna Walewska, Agnieszka Ishikawa, Takao Szczepańska, Katarzyna Ajduk, Anna |
author_facet | Czajkowska, Katarzyna Walewska, Agnieszka Ishikawa, Takao Szczepańska, Katarzyna Ajduk, Anna |
author_sort | Czajkowska, Katarzyna |
collection | PubMed |
description | Maternal aging affects various aspects of oocytes’ physiology, including the functionality of their nuclear apparatus and mitochondria. In the present paper, we wished to investigate whether advanced reproductive age impacts oocytes’ ability to generate proper Ca(2+) oscillations in response to monospermic fertilization. We examined three different mouse strains/crosses: inbred C57BL/6Tar, outbred Tar:SWISS, and hybrid F1 (C57BL/6Tar × CBA/Tar). The females were either 2–4 months old (young) or 13–16 months old (aged). We observed that the Ca(2+) oscillatory pattern is altered in a strain-dependent manner and changes were more profound in aged C57BL/6Tar and F1 than in aged Tar:SWISS oocytes. We also showed that maternal aging differently affects the size of Ca(2+) store and expression of Itpr1, Atp2a2, Erp44, and Pdia3 genes involved in Ca(2+) homeostasis in oocytes of C57BL/6Tar, Tar:SWISS, and F1 genetic background, which may explain partially the differences in the extent of age-dependent changes in the Ca(2+) oscillations in those oocytes. Maternal aging did not have any visible impact on the distribution of the ER cisterns in oocytes of all three genetic types. Finally, we showed that maternal aging alters the timing of the first embryonic interphase onset and that this timing correlates in C57BL/6Tar and Tar:SWISS oocytes with the frequency of fertilization-induced Ca(2+) oscillations. Our results indicate that extreme caution is required when conclusions about oocyte/embryo physiological response to aging are made and complement an increasing amount of evidence that mammalian (including human) susceptibility to aging differs greatly depending on the genetic background of the individual. |
format | Online Article Text |
id | pubmed-7609943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-76099432020-11-09 Age-related alterations in fertilization-induced Ca(2+) oscillations depend on the genetic background of mouse oocytes(†) Czajkowska, Katarzyna Walewska, Agnieszka Ishikawa, Takao Szczepańska, Katarzyna Ajduk, Anna Biol Reprod Research Article Maternal aging affects various aspects of oocytes’ physiology, including the functionality of their nuclear apparatus and mitochondria. In the present paper, we wished to investigate whether advanced reproductive age impacts oocytes’ ability to generate proper Ca(2+) oscillations in response to monospermic fertilization. We examined three different mouse strains/crosses: inbred C57BL/6Tar, outbred Tar:SWISS, and hybrid F1 (C57BL/6Tar × CBA/Tar). The females were either 2–4 months old (young) or 13–16 months old (aged). We observed that the Ca(2+) oscillatory pattern is altered in a strain-dependent manner and changes were more profound in aged C57BL/6Tar and F1 than in aged Tar:SWISS oocytes. We also showed that maternal aging differently affects the size of Ca(2+) store and expression of Itpr1, Atp2a2, Erp44, and Pdia3 genes involved in Ca(2+) homeostasis in oocytes of C57BL/6Tar, Tar:SWISS, and F1 genetic background, which may explain partially the differences in the extent of age-dependent changes in the Ca(2+) oscillations in those oocytes. Maternal aging did not have any visible impact on the distribution of the ER cisterns in oocytes of all three genetic types. Finally, we showed that maternal aging alters the timing of the first embryonic interphase onset and that this timing correlates in C57BL/6Tar and Tar:SWISS oocytes with the frequency of fertilization-induced Ca(2+) oscillations. Our results indicate that extreme caution is required when conclusions about oocyte/embryo physiological response to aging are made and complement an increasing amount of evidence that mammalian (including human) susceptibility to aging differs greatly depending on the genetic background of the individual. Oxford University Press 2020-08-06 /pmc/articles/PMC7609943/ /pubmed/32761132 http://dx.doi.org/10.1093/biolre/ioaa139 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Czajkowska, Katarzyna Walewska, Agnieszka Ishikawa, Takao Szczepańska, Katarzyna Ajduk, Anna Age-related alterations in fertilization-induced Ca(2+) oscillations depend on the genetic background of mouse oocytes(†) |
title | Age-related alterations in fertilization-induced Ca(2+) oscillations depend on the genetic background of mouse oocytes(†) |
title_full | Age-related alterations in fertilization-induced Ca(2+) oscillations depend on the genetic background of mouse oocytes(†) |
title_fullStr | Age-related alterations in fertilization-induced Ca(2+) oscillations depend on the genetic background of mouse oocytes(†) |
title_full_unstemmed | Age-related alterations in fertilization-induced Ca(2+) oscillations depend on the genetic background of mouse oocytes(†) |
title_short | Age-related alterations in fertilization-induced Ca(2+) oscillations depend on the genetic background of mouse oocytes(†) |
title_sort | age-related alterations in fertilization-induced ca(2+) oscillations depend on the genetic background of mouse oocytes(†) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609943/ https://www.ncbi.nlm.nih.gov/pubmed/32761132 http://dx.doi.org/10.1093/biolre/ioaa139 |
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