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Probing of breast cancer using a combination of plasma and urinary circulating cell-free DNA
Monitoring of early-stage breast cancer is critical in promptly addressing disease relapse. Circulating cell-free DNA provides a minimally invasive and sensitive means to probing the disease. In a longitudinal analysis of 250 patients with early breast cancer, we compared the circulating cell-free D...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610146/ https://www.ncbi.nlm.nih.gov/pubmed/33044511 http://dx.doi.org/10.1042/BSR20194306 |
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author | Zuo, Zhigang Tang, Jiying Cai, Xiaojun Ke, Feng Shi, Zhenzong |
author_facet | Zuo, Zhigang Tang, Jiying Cai, Xiaojun Ke, Feng Shi, Zhenzong |
author_sort | Zuo, Zhigang |
collection | PubMed |
description | Monitoring of early-stage breast cancer is critical in promptly addressing disease relapse. Circulating cell-free DNA provides a minimally invasive and sensitive means to probing the disease. In a longitudinal analysis of 250 patients with early breast cancer, we compared the circulating cell-free DNA recovered from both plasma and urine specimens. For comparison, 50 healthy controls were also recruited. Specific mutations associated with the disease were profiled to determine the clinical sensitivity and specificity. Correlations of recovered concentrations of cell-free DNA with outcomes were examined to address early prognostication. PIK3CA mutation profiling in both plasma and urinary cell-free DNA showed an agreement of 97.2% compared with the results obtained for tumor tissues. The analysis of healthy controls revealed that cell-free DNA measurements were stable and consistent over time. Over the short 6-month period of monitoring, our analyses showed declines in recovered cell-free DNA; these findings may aid physicians in stratifying patients at higher risk for relapse. Similar results were observed in both plasma and urine specimens (hazard ratios: 2.16 and 2.48, respectively). Cell-free DNA presents a novel and sensitive method for the monitoring of early-stage breast cancer. In the present study, serial measurements of both plasma and urine specimens were useful in probing the disease. |
format | Online Article Text |
id | pubmed-7610146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76101462020-11-10 Probing of breast cancer using a combination of plasma and urinary circulating cell-free DNA Zuo, Zhigang Tang, Jiying Cai, Xiaojun Ke, Feng Shi, Zhenzong Biosci Rep Cancer Monitoring of early-stage breast cancer is critical in promptly addressing disease relapse. Circulating cell-free DNA provides a minimally invasive and sensitive means to probing the disease. In a longitudinal analysis of 250 patients with early breast cancer, we compared the circulating cell-free DNA recovered from both plasma and urine specimens. For comparison, 50 healthy controls were also recruited. Specific mutations associated with the disease were profiled to determine the clinical sensitivity and specificity. Correlations of recovered concentrations of cell-free DNA with outcomes were examined to address early prognostication. PIK3CA mutation profiling in both plasma and urinary cell-free DNA showed an agreement of 97.2% compared with the results obtained for tumor tissues. The analysis of healthy controls revealed that cell-free DNA measurements were stable and consistent over time. Over the short 6-month period of monitoring, our analyses showed declines in recovered cell-free DNA; these findings may aid physicians in stratifying patients at higher risk for relapse. Similar results were observed in both plasma and urine specimens (hazard ratios: 2.16 and 2.48, respectively). Cell-free DNA presents a novel and sensitive method for the monitoring of early-stage breast cancer. In the present study, serial measurements of both plasma and urine specimens were useful in probing the disease. Portland Press Ltd. 2020-11-03 /pmc/articles/PMC7610146/ /pubmed/33044511 http://dx.doi.org/10.1042/BSR20194306 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Cancer Zuo, Zhigang Tang, Jiying Cai, Xiaojun Ke, Feng Shi, Zhenzong Probing of breast cancer using a combination of plasma and urinary circulating cell-free DNA |
title | Probing of breast cancer using a combination of plasma and urinary circulating cell-free DNA |
title_full | Probing of breast cancer using a combination of plasma and urinary circulating cell-free DNA |
title_fullStr | Probing of breast cancer using a combination of plasma and urinary circulating cell-free DNA |
title_full_unstemmed | Probing of breast cancer using a combination of plasma and urinary circulating cell-free DNA |
title_short | Probing of breast cancer using a combination of plasma and urinary circulating cell-free DNA |
title_sort | probing of breast cancer using a combination of plasma and urinary circulating cell-free dna |
topic | Cancer |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610146/ https://www.ncbi.nlm.nih.gov/pubmed/33044511 http://dx.doi.org/10.1042/BSR20194306 |
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