Cargando…
Optimizing imaging speed and excitation intensity for single molecule localization microscopy
High laser powers are common practice in single molecule localization microscopy (SMLM) to speed up data acquisition. Here, we systematically quantified how excitation intensity influences localization precision and labeling density, the two main factors determining data quality. We found a strong t...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610360/ https://www.ncbi.nlm.nih.gov/pubmed/32807954 http://dx.doi.org/10.1038/s41592-020-0918-5 |