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The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection
Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN-I). What other functions pDCs exert in vivo during viral infections is controversial and more studies are needed to understand their orchestration. Here, we characterize in-depth and link pDC activation states in animal...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610367/ https://www.ncbi.nlm.nih.gov/pubmed/32690951 http://dx.doi.org/10.1038/s41590-020-0731-4 |
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author | Abbas, Abdenour Manh, Thien-Phong Vu Valente, Michael Collinet, Nils Attaf, Noudjoud Dong, Chuang Naciri, Karima Chelbi, Rabie Brelurut, Geoffray Cervera-Marzal, Inaki Rauwel, Benjamin Davignon, Jean-Luc Bessou, Gilles Thomas-Chollier, Morgane Thieffry, Denis Villani, Alexandra-Chloé Milpied, Pierre Dalod, Marc Tomasello, Elena |
author_facet | Abbas, Abdenour Manh, Thien-Phong Vu Valente, Michael Collinet, Nils Attaf, Noudjoud Dong, Chuang Naciri, Karima Chelbi, Rabie Brelurut, Geoffray Cervera-Marzal, Inaki Rauwel, Benjamin Davignon, Jean-Luc Bessou, Gilles Thomas-Chollier, Morgane Thieffry, Denis Villani, Alexandra-Chloé Milpied, Pierre Dalod, Marc Tomasello, Elena |
author_sort | Abbas, Abdenour |
collection | PubMed |
description | Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN-I). What other functions pDCs exert in vivo during viral infections is controversial and more studies are needed to understand their orchestration. Here, we characterize in-depth and link pDC activation states in animals infected by mouse cytomegalovirus, by combining Ifnb1 reporter mice with flow cytometry, single-cell RNA sequencing, confocal microscopy and a cognate CD4 T cell activation assay. We show that IFN-I production and T cell activation were performed by the same pDC, but sequentially in time and in different micro-anatomical locations. In addition, we show that pDC commitment to IFN-I production was marked early on by their downregulation of LIFR and promoted by cell-intrinsic TNF signaling. We propose a novel model of how individual pDCs are endowed to exert different functions in vivo during a viral infection in a manner tightly orchestrated in time and space. |
format | Online Article Text |
id | pubmed-7610367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-76103672021-03-19 The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection Abbas, Abdenour Manh, Thien-Phong Vu Valente, Michael Collinet, Nils Attaf, Noudjoud Dong, Chuang Naciri, Karima Chelbi, Rabie Brelurut, Geoffray Cervera-Marzal, Inaki Rauwel, Benjamin Davignon, Jean-Luc Bessou, Gilles Thomas-Chollier, Morgane Thieffry, Denis Villani, Alexandra-Chloé Milpied, Pierre Dalod, Marc Tomasello, Elena Nat Immunol Article Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN-I). What other functions pDCs exert in vivo during viral infections is controversial and more studies are needed to understand their orchestration. Here, we characterize in-depth and link pDC activation states in animals infected by mouse cytomegalovirus, by combining Ifnb1 reporter mice with flow cytometry, single-cell RNA sequencing, confocal microscopy and a cognate CD4 T cell activation assay. We show that IFN-I production and T cell activation were performed by the same pDC, but sequentially in time and in different micro-anatomical locations. In addition, we show that pDC commitment to IFN-I production was marked early on by their downregulation of LIFR and promoted by cell-intrinsic TNF signaling. We propose a novel model of how individual pDCs are endowed to exert different functions in vivo during a viral infection in a manner tightly orchestrated in time and space. 2020-09-01 2020-07-20 /pmc/articles/PMC7610367/ /pubmed/32690951 http://dx.doi.org/10.1038/s41590-020-0731-4 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Abbas, Abdenour Manh, Thien-Phong Vu Valente, Michael Collinet, Nils Attaf, Noudjoud Dong, Chuang Naciri, Karima Chelbi, Rabie Brelurut, Geoffray Cervera-Marzal, Inaki Rauwel, Benjamin Davignon, Jean-Luc Bessou, Gilles Thomas-Chollier, Morgane Thieffry, Denis Villani, Alexandra-Chloé Milpied, Pierre Dalod, Marc Tomasello, Elena The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection |
title | The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection |
title_full | The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection |
title_fullStr | The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection |
title_full_unstemmed | The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection |
title_short | The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection |
title_sort | activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610367/ https://www.ncbi.nlm.nih.gov/pubmed/32690951 http://dx.doi.org/10.1038/s41590-020-0731-4 |
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