Up to 100-fold speedup and multiplexing in optimized DNA-PAINT

DNA-PAINT’s imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed-limit and importantly is only applicable to imaging single targets. To further improve acquisition speed, we here intr...

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Detalles Bibliográficos
Autores principales: Strauss, Sebastian, Jungmann, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610413/
https://www.ncbi.nlm.nih.gov/pubmed/32601424
http://dx.doi.org/10.1038/s41592-020-0869-x
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author Strauss, Sebastian
Jungmann, Ralf
author_facet Strauss, Sebastian
Jungmann, Ralf
author_sort Strauss, Sebastian
collection PubMed
description DNA-PAINT’s imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed-limit and importantly is only applicable to imaging single targets. To further improve acquisition speed, we here introduce concatenated, periodic DNA sequence motifs, yielding up to 100-fold faster sampling compared to traditional DNA-PAINT. We extend this approach to six orthogonal sequence motifs, now enabling speed-optimized multiplexed imaging.
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spelling pubmed-76104132021-03-23 Up to 100-fold speedup and multiplexing in optimized DNA-PAINT Strauss, Sebastian Jungmann, Ralf Nat Methods Article DNA-PAINT’s imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed-limit and importantly is only applicable to imaging single targets. To further improve acquisition speed, we here introduce concatenated, periodic DNA sequence motifs, yielding up to 100-fold faster sampling compared to traditional DNA-PAINT. We extend this approach to six orthogonal sequence motifs, now enabling speed-optimized multiplexed imaging. 2020-08-01 2020-06-29 /pmc/articles/PMC7610413/ /pubmed/32601424 http://dx.doi.org/10.1038/s41592-020-0869-x Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Strauss, Sebastian
Jungmann, Ralf
Up to 100-fold speedup and multiplexing in optimized DNA-PAINT
title Up to 100-fold speedup and multiplexing in optimized DNA-PAINT
title_full Up to 100-fold speedup and multiplexing in optimized DNA-PAINT
title_fullStr Up to 100-fold speedup and multiplexing in optimized DNA-PAINT
title_full_unstemmed Up to 100-fold speedup and multiplexing in optimized DNA-PAINT
title_short Up to 100-fold speedup and multiplexing in optimized DNA-PAINT
title_sort up to 100-fold speedup and multiplexing in optimized dna-paint
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610413/
https://www.ncbi.nlm.nih.gov/pubmed/32601424
http://dx.doi.org/10.1038/s41592-020-0869-x
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